热带海洋学报 ›› 2016, Vol. 35 ›› Issue (3): 72-78.doi: 10.11978/2015056

• 海洋生物学 • 上一篇    下一篇

温度胁迫及恢复初期稀杯盔形珊瑚共生虫黄藻Hsp70、Hsp90、psaA、psbA基因表达分析

沈城1, 2, 刘楚吾1, 刘丽1   

  1. 1. 广东海洋大学水产学院, 南海水产经济动物增养殖广东省普通高校重点实验室, 广东 湛江 524088;
    2. 广东徐闻珊瑚礁国家级自然保护区管理局, 广东 徐闻 524100
  • 收稿日期:2015-04-24 出版日期:2016-05-29 发布日期:2016-05-27
  • 通讯作者: 刘丽, 教授, 主要从事水产经济动物发育生物学研究。E-mail: zjouliuli@163.com
  • 作者简介:作者简介:沈城(1988—), 男, 硕士研究生, 从事水产经济动物增养殖学研究。E-mail: shencheng55045785@126.com
  • 基金资助:
    广东省海洋渔业科技推广专项(A201308E02); 广东省自然科学基金项目(S2011010000269)

Heat-induced stress genes Hsp70, Hsp90 and chloroplast psaA and psbA gene expressions of Galaxea astreata endosymbiotic zooxanthella revealing the ability of tolerance and recovery

SHEN Cheng1, 2, LIU Chuwu1, LIU Li1   

  1. 1. Fisheries College, Guangdong Ocean University, Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal, Regular High Education Institution of Guangdong Province, Zhanjiang 524088, China;
    2. Xuwen National Coral Reef Nature Reserve, Xuwen 524100, China
  • Received:2015-04-24 Online:2016-05-29 Published:2016-05-27
  • Contact: LIU Li. E-mail: zjouliuli@163.com
  • Supported by:
    Marine Fishery Science and Technology Promotion Project in Guangdong Province (A201308E02); Guangdong Natural Science Foundation Project (S2011010000269)

摘要: 以SAM (S-腺苷酰-L-甲硫氨酸)基因作为内参基因, 利用实时荧光定量PCR技术对稀杯盔形珊瑚(Galaxea astreata)共生虫黄藻(Symbiodinium spp.) Hsp70、Hsp90、psaApsbA基因在升温至32℃持续胁迫及恢复至28℃后的表达情况进行了研究。结果表明, 升温至32℃时, Hsp70、Hsp90表达量无显著变化(p>0.05), 随着高温胁迫的持续, Hsp70Hsp90的表达量显著增高(p<0.01), 到32℃ 7d时2个基因表达量达到最大值(分别为胁迫前的10.3倍和28.2倍); 在随后的降温过程中, Hsp70Hsp90的表达量显著下降(p<0.05), 当温度恢复至28℃ 5d时2个基因的表达量均下降至较低水平(分别为胁迫前的0.6倍和2.0倍, p<0.01), 且趋于稳定。随着温度的升高, psaA的表达量略有升高(p>0.05), 在31℃ 24h时表达量达到最高(为胁迫前的1.2倍), 随后显著下降(p<0.01); 而psbA的表达量随着温度的升高显著增加(p<0.05); 在31℃ 24h时表达量达到最高(为胁迫前的1.9倍), 继续升温至32℃持续胁迫24h后才开始显著下降(p<0.01)。当温度降至28℃时, 2个基因的表达量有一定程度的增加, 但是仍显著低于胁迫前的表达水平(p<0.01), 28℃ 9d时分别为胁迫前的0.27倍和0.15倍, 表明热应激可能使虫黄藻的光合功能受到损伤。

关键词: 虫黄藻, 基因表达, 温度胁迫

Abstract: The relative expressions of stress genes Hsp70, Hsp90 and choloplast psaA, psbA (SAM as reference gene) in Galaxea astreata endosymbiotic zooxanthella (Symbiodinium spp.) under heat stress were detected by using real-time PCR technique. All samples were exposed to elevated temperature at 32 ℃ over a 7-day period, and then the temperature was reduced to 28 ℃ gradually, with the temperature change from 28 ℃ to 32 ℃ at a rate of 1 ℃ per 24 hours. The results showed that the expression patterns of Hsp70 and Hsp90 were similar. The induced expression levels were upregulated but showed no significant difference compared to the control group (p>0.05) during the early stage of temperature climbing, subsequently reaching the maximize level after 168h tolerance period at 32 ℃. The lowest abundance of the gene products was detected when returned to the normal condition of 28 ℃ after 5-day recovery. Moreover, we examined the expression levels of chloroplast psaA and psbA genes stimulated by heat. Both were increased at the initial stage of thermal stress, and reached maximize at 31 ℃ for 24 h, with 1.2 and 1.9 folds compared to the level of the untreated group, respectively. Subsequently at 32 ℃, psbA transcripts maintained the high level until 24 h consecutive hours of stress, while psaA immediately decreased significantly (p<0.01). When temperature recovery reached 28 ℃, the transcripts of the two genes were significantly lower than the initial level (p<0.01), which indicates that the photosynthetic function of endosymbiotic zooxanthella may be injured (or inhibited).

Key words: zooxanthella, gene expression, thermal stress

中图分类号: 

  • Q949.2