热带海洋学报 ›› 2021, Vol. 40 ›› Issue (4): 22-34.doi: 10.11978/2020067

• 海洋生物学 • 上一篇    下一篇

两株溶藻弧菌噬菌体的生理特性和基因组研究

钟宛宣1,2(), 杨芸兰3, 李祥付1,4, 徐杰1,2,4()   

  1. 1.中国科学院南海海洋研究所, 热带海洋环境国家重点实验室, 广东 广州 510301
    2.中国科学院大学海洋学院, 北京 100049
    3.厦门大学环境与生态学院, 近海海洋环境科学国家重点实验室, 福建 厦门 361102
    4.中国科学院南海生态环境工程创新研究院, 广东 广州 510301
  • 收稿日期:2020-06-24 修回日期:2020-07-26 出版日期:2021-07-10 发布日期:2020-07-29
  • 通讯作者: 徐杰
  • 作者简介:钟宛宣(1995—), 女, 广东省广州市人, 硕士研究生。email: zhongwanxuan17@mails.ucas.ac.cn
  • 基金资助:
    广州市科技计划项目(20190420029);国家自然科学基金(41506095);国家自然科学基金(41676075);中国科学院南海生态环境工程创新研究院自主部署项目(ISEE2019ZR02);广东省省级科技计划项目(2018B030320005);中国博士后科学基金(2019M662237)

Physiological and genomic characteristics of two lytic phages infecting Vibrio alginolyticus

ZHONG Wanxuan1,2(), YANG Yunlan3, LI Xiangfu1,4, XU Jie1,2,4()   

  1. 1. State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
    2. College of Marine Science, University of Chinese Academy of Sciences, Beijing 100049, China
    3. College of the Environment and Ecology, State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen, 361102 China
    4. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, 510301 China
  • Received:2020-06-24 Revised:2020-07-26 Online:2021-07-10 Published:2020-07-29
  • Contact: XU Jie
  • Supported by:
    Project of Program of Science and Technology of Guangzhou(20190420029);National Natural Science Foundation of China(41506095);National Natural Science Foundation of China(41676075);Project of Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences(ISEE2019ZR02);Project of Department of Science and Technology of Guangdong Province(2018B030320005);China Postdoctoral Science Foundation(2019M662237)

摘要:

利用噬菌体治疗水产养殖细菌病害具有专一性强、自我增殖能力强等优点, 可以有效减少对环境的污染。为了获得可防治水产病原体溶藻弧菌(Vibrio alginolyticus)的生物杀菌剂, 本研究以Vibrio alginolyticus ATCC 17749T为宿主, 从福建省东山岛的对虾养殖场与水产市场污水环境中分离得到两株巨型噬菌体vB_ValM_R10Z和vB_ValM_R11Z, 并分别从噬菌体形态、宿主范围、生命周期和基因组信息等方面进行了鉴定和分析。实验结果显示, 噬菌体R10Z和R11Z具有相似的噬菌斑形态和电镜形态, 属于肌尾噬菌体; 除宿主之外, 噬菌体R10Z和R11Z均能侵染另一株欧文氏弧菌Vibrio owensii JL3186; 两株噬菌体均对氯仿不敏感, 表明其衣壳蛋白不含脂类物质; 噬菌体R10Z和R11Z的潜伏期均为20min, 但裂解量却相差一倍, 分别为45PFU·cell-1和114PFU·cell-1; 两株噬菌体的基因组大小分别为247167bp和246831bp, G+C含量分别为41.30%和41.33%, 两者基因组相似性达99.45%, 均未检测出毒力基因与抗药性基因; 系统发育分析显示两株噬菌体均属于Myoviridae(科), Tevenvirinae(亚科), Schizotequatrovirus(属), 同源基因在环境中分布广泛。两株噬菌体性状优良, 在水产病害的噬菌体治疗领域具有重要的研究价值和潜在的应用价值。

关键词: 弧菌, 噬菌体, 噬菌体治疗, 生理特性, 基因组

Abstract:

Phage therapy of bacterial diseases was considered to be effective and environmentally friendly in aquaculture. To obtain the biological agents that prevent Vibro alginolyticus, two jumbo phages vB_ValM_R10Z and vB_ValM_R11Z were isolated from prawn farming waters and the sewage in fishery markets in Dongshan Island, Fujian Province, using Vibrio alginolyticus ATCC 17749 T as the host. The morphology, host range, life cycle, and genome were identified and analyzed. Phages R10Z and R11Z had similar morphology of plaques and virions, and shared the same host range of infecting other V. owensii strain JL3186. Both phages were not sensitive to chloroform, indicating that the capsid did not contain lipids. They had the same latent period of 20 min but different blast sizes of 45 PFU·cell -1 for R10Z and 114 PFU·cell-1 for R11Z. The genomic sizes of phages R10Z and R11Z were 247167 bp and 246831 bp with G+C content of 41.30% and 41.33%, respectively. Furthermore, their genomes had a similarity of 99.45%, without virulence genes and antibiotic resistance genes. Our phylogenetic analysis showed that they belonged to genus Schizotequatrovirus, subfamily Tevenvirinae, family Myovirdae. Their homologous genes were distributed in the environment widely. Phages R10Z and R11Z had good capability and great potentials for their usage as phage therapy agents of vibriosis in aquaculture.

Key words: Vibrio, phage, phage therapy, physiological properties, genome

中图分类号: 

  • P735.531