热带海洋学报 ›› 2021, Vol. 40 ›› Issue (6): 135-139.doi: 10.11978/2020103

• 海洋生物学 • 上一篇    

中国南海软珊瑚附生真菌Acremonium sp. SCSIO41216的次级代谢产物研究

张涵1,2(), 谭雁鸿1, 杨斌2,3, 刘永宏2,3(), 李云秋1()   

  1. 1.桂林医学院, 广西 桂林 541004
    2.中国科学院南海海洋研究所热带海洋生物资源与生态重点实验室, 广东 广州 510301
    3.南方海洋科学与工程广东省实验室(广州), 广东 广州 511457
  • 收稿日期:2020-09-14 修回日期:2020-11-16 出版日期:2021-11-10 发布日期:2020-11-19
  • 通讯作者: 刘永宏,李云秋
  • 作者简介:张涵(1994—), 硕士, 从事天然药物化学研究, email: zhanghanmarc@163.com
  • 基金资助:
    国家自然科学基金地区基金(81860626);广州市科技计划项目(201804010462);广东省自然科学基金项目(2018A0303130219)

Study on the secondary metabolites from the South China Sea soft coral-derived fungus Acremonium sp. SCSIO41216

ZHANG Han1,2(), TAN Yanhong1, YANG Bin2,3, LIU Yonghong2,3(), LI Yunqiu1()   

  1. 1. College of Pharmacy, Guilin Medical University, Guilin 541004, China
    2. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
    3. Guangdong Provincial Laboratory of Marine Science and Engineering of South China (Guangzhou), Guangdong Guangzhou, 511457
  • Received:2020-09-14 Revised:2020-11-16 Online:2021-11-10 Published:2020-11-19
  • Contact: LIU Yonghong,LI Yunqiu
  • Supported by:
    National Natural Science Foundation of China(81860626);Guangzhou Science and Technology Project(201804010462);Natural Science Foundation of Guangdong Province(2018A0303130219)

摘要:

采用硅胶柱层析色谱法、中压正相柱层析色谱法、中压反相柱层析色谱法以及半制备高效液相色谱法对中国南海软珊瑚来源真菌Acremonium sp. SCSIO41216的大米发酵产物进行分离纯化, 并使用核磁共振、质谱等波谱学方法, 结合其理化性质及文献数据鉴定了7个单体化合物结构: fischexanthone(1)、sydowinin A (2)、(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (3)、16-O-去乙酰基夫西地酸内酯(4)、环-(S)-脯氨酸-(R)-亮氨酸(5)、环-(S)-脯氨酸-8-羟基-(R)-异亮氨酸(6)和环(L)-脯氨酸-(L)-苯丙氨酸(7)。化合物1—7均首次从海洋软珊瑚来源的枝顶孢属真菌(Acremonium sp.)中分离获得。

关键词: 南海, 软珊瑚附生真菌, Acremonium sp., 次级代谢产物

Abstract:

The rice fermentation products of the soft coral-derived fungus Acremonium sp. SCSIO41216 from the South China Sea were isolated and purified by silica gel column, medium pressure preparative liquid chromatography (MPLC), ostade-cylsilane (ODS), and semipreparative high Performance Liquid Chromatography (HPLC). The structures of the isolated compounds were identified by nuclear magnetic resonance (NMR)and mass spectrum (MS), and compared with the published data. Seven known compounds were obtained and identified as fischexanthone (1), sydowinin A (2), (22E)-5α,8α- epidioxyergosta-6,22-dien-3β-ol (3), 16-deacetylfusidic acid lactone (4), cyclo-((S)-Pro-(R)-Leu) (5), cyclo-(S)-Pro-8- hydroxy-(R)-Ile (6), and cyclo (L)-Pro-(L)-Phe (7). Compounds 1-7 were isolated from Acremonium sp. derived from marine soft coral for the first time.

Key words: South China Sea, soft coral, Acremonium sp., secondary metabolites

中图分类号: 

  • R282.77