热带海洋学报 ›› 2021, Vol. 40 ›› Issue (6): 93-101.doi: 10.11978/2020146

• 海洋生物学 • 上一篇    下一篇

含铁肠杆菌素受体调节蛋白VPA0148对副溶血弧菌毒力的影响

朱馨媛(), 刘敏, 黄颖, 赵哲()   

  1. 河海大学海洋学院海洋生物系, 江苏 南京 210098
  • 收稿日期:2020-12-14 修回日期:2021-02-22 出版日期:2021-11-10 发布日期:2021-02-25
  • 通讯作者: 赵哲
  • 作者简介:朱馨媛(1996—), 女, 江苏省南京市人, 硕士研究生, 从事副溶血弧菌毒力调控相关研究。email: xyzhu@hhu.edu.cn
  • 基金资助:
    国家自然科学基金项目(31872597);江苏省农业科技自主创新资金项目(CX[19]2033);江苏省现代农业产业技术体系项目(JATS[2020]465)

Effects of the ferric enterobactin receptor regulator VPA0148 on virulence of Vibrio parahaemolyticus

ZHU Xinyuan(), LIU Min, HUANG Ying, ZHAO Zhe()   

  1. Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China
  • Received:2020-12-14 Revised:2021-02-22 Online:2021-11-10 Published:2021-02-25
  • Contact: ZHAO Zhe
  • Supported by:
    National Natural Science Foundation of China(31872597);Jiangsu Agriculture Science and Technology Innovation Fund(CX[19]2033);Earmarked Fund for Jiangsu Agricultural Industry Technology System(JATS[2020]465)

摘要:

副溶血弧菌(Vibrio parahaemolyticus)是一种嗜盐性革兰氏阴性菌, 广泛分布于海洋环境中, 能够引起肠胃炎、伤口感染以及败血症, 是人类重要的病原菌之一。宿主体内被认为是一种低铁环境, 能够激发致细菌的毒力。副溶血弧菌能够分泌弧菌素或利用外源铁载体来获取铁。在低铁条件下, 编码双组份调控系统的基因簇VPA0148-VPA0149转录上调, 使含铁肠杆菌素受体PeuA转录出有活性的蛋白。VPA0148编码产物为一个响应调节因子, 具有一个磷酸基团接收结构域和一个DNA结合结构域。本研究发现, VPA0148的缺失增强了菌株的生物膜形成能力和对斑马鱼的致死能力, 同时能够促进菌株在低铁环境中的生长, 并增强菌株的群集运动。研究结果表明含铁肠杆菌素受体调节蛋白VPA0148对副溶血弧菌致病力具有调控作用, 增进了对铁调节副溶血弧菌毒力机制的认识。

关键词: 副溶血弧菌, 含铁肠杆菌素受体调节蛋白, 毒力, 运动能力, 生物膜

Abstract:

Vibrio parahaemolyticus is a halophilic Gram-negative bacterium originally inhabiting marine environment. It is one of the primary human pathogenic bacteria, which causes gastroenteritis, wound infections and septicemia. The host environment is low-iron conditions, thus capable of inducing the virulence of pathogens. V. parahaemolyticus utilizes vibriocin or heterogenous siderophore to obtain iron. The transcription of gene cluster VPA0148-VPA0149 (encoding a two-component regulatory system) is upregulated under iron-limiting conditions, which increases the active transcriptional form of ferric enterobactin receptor gene peuA. VPA0148 encodes a response regulator containing a phosphoacceptor receiver (REC) domain and a DNA-binding domain. In this study, the deletion of VPA0148 enhanced the lethality to zebrafish as well as biofilm formation in V. parahaemolyticus. Moreover, under iron-limiting conditions, the growth and swarming motility were improved in VPA0148 deletion mutant. The results indicate that VPA0148 contributes to regulating the pathogenesis of V. parahaemolyticus. This study provides an important insight for further understanding the mechanism of how iron regulates virulence of V. parahaemolyticus.

Key words: Vibrio parahaemolyticus, VPA0148, virulence, swarming, biofilm

中图分类号: 

  • S941.42