热带海洋学报

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海洋放线菌Nocardiopsis dassonvillei JS106的抗群体感应活性物质研究

缪莉, 钱嘉兴, 莫杰, 周恒, 钱生辉, 董昆明   

  1. 扬州大学环境科学与工程学院, 江苏 扬州 225127

  • 收稿日期:2022-02-13 修回日期:2022-04-23 出版日期:2022-04-28 发布日期:2022-04-28
  • 通讯作者: 董昆明
  • 基金资助:
    广东省海洋药物重点实验室开放课题基金及中国科学院热带海洋生物资源与生态重点实验室开放课题 (2018011008)

Anti-quorum sensing active substances from a marine-derived actinobacterium Nocardiopsis dassonvillei JS106

MIAO Li, QIAN Jiaxing, MO Jie, ZHOU Heng, QIAN Shenghui, DONG Kuming   

  1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China

  • Received:2022-02-13 Revised:2022-04-23 Online:2022-04-28 Published:2022-04-28
  • Contact: Kunming DONG
  • Supported by:

    Open Project of Key Laboratory of Marine Medicine, Guangdong Province and Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences (2018011008)

摘要: 群体感应抑制剂能够通过调控致病菌的群体感应系统来降低其致病力,且不易使其产生抗性突变,有望治疗或协同治疗细菌性感染,以应对细菌耐药性问题。本文研究一株海洋来源的放线菌达松维尔拟诺卡氏菌JS106中的抗群体感应活性物质。采用减压柱层析和高效液相色谱从JS106的发酵液中分离到6个化合物,1,6-二羟基吩嗪(1)、6-羟基-1-甲氧基吩嗪(2)、4',7-二甲氧基异黄酮(3)、N-(2-羟基苯基)乙酰胺(4)、1-甲氧基吩嗪(5)和2-氨基吩恶嗪-3-酮(6)。在测试浓度下,所有化合物均表现出很好的抗群体感应活性,对紫色色杆菌的色素产生有显著的抑制作用且没有明显的生长抑制。菌株JS106可产生丰富的吩嗪类化合物,在抗群体感应方面有出色的应用潜力。

关键词: 海洋放线菌, 生物活性, 抗群体感应活性, 拟诺卡氏菌, 吩嗪类化合物

Abstract: Quorum sensing inhibitors (QSIs) could regulate the pathogenicity by mediating the bacterial quorum sensing system, and it is not easy to cause resistance mutations. To deal with bacterial drug resistance, QSIs might be useful in treating or cooperated treating bacterial infections. In this study, the anti-quorum sensing substances from a marine derived actinobacterium Nocardiopsis dassonvillei JS106 was investigated. Six compounds, 1,6- dihydroxyphenazine(1), 6-hydroxy-1-methoxyphenazine(2), 4',7-dimethoxyisoflavone(3), N-(2-hydroxyphenyl) acetamide(4), 1-methoxyphenazine(5) and 2-aminophenoxazin-3-one(6), were isolated from the spent culture medium of JS106 using VLC and HPLC. All of the pure compounds showed significant anti-quorum sensing activity that inhibited the violacein production of Chromobacterium violaceum ATCC 12472 without visible growth inhibition at the test concentrations. N. dassonvillei JS106 could produce many phenazines and has better potential in anti-quorum sensing application.

Key words: marine actinomycetes, quorum sensing, anti-quorum sensing activity, Nocardiopsis dassonvillei, phenazines