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热带海洋学报

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中国南海来源真菌Penicillium sp. SCSIO 40438次级代谢产物研究

朱义广1, 2, 3，牟鹏云1, 4，张庆波1, 2, 3，张长生1, 2, 3


基金项目：广东省自然资源厅项目（粤自然资合[2021]48号）；中国科学院王宽诚率先人才计划“卢嘉锡国际团队项目”（GJTD-2020-12）；广东省重点领域研发计划项目（2020B1111030005）；广东省科学技术厅项目（2019TQ05H299）

作者简介：朱义广（1978-），男，山东省济宁市人，研究员，从事天然产物生物合成研究。email: ygzhu@scsio.ac.cn

通信作者：朱义广，email: ygzhu@scsio.ac.cn；牟鹏云，email: Moupengyun@163.com

Foundation item: Guangdong MEPP Funds (GDNRC[2021]48); K.C.Wong Education Foundation (GJTD-2020-12); Key-Area Research and Development Program of Guangdong Province (2020B1111030005); Special Support Program for Training High-Level Talents in Guangdong (2019TQ05H299)

Corresponding author: ZHU Yiguang. email: ygzhu@scsio.ac.cn; MOU Pengyun. email: Moupengyun@163.com

  

  1. 1中国科学院南海海洋研究所，中国科学院热带海洋生物资源与生态重点实验室，广东省海洋药物重点实验室，广东 广州 510301

    2中国科学院大学，北京 100049

    3南方海洋科学与工程广东省实验室（广州），广东 广州 511458

    4塔里木大学生命科学与技术学院，新疆 阿拉尔 843300

  • 收稿日期:2022-04-14 修回日期:2022-05-08 出版日期:2022-05-10 发布日期:2022-05-10
  • 通讯作者: Qingbo Zhang
  • 基金资助:
    广东省自然资源厅项目（粤自然资合[2021]48号）；中国科学院王宽诚率先人才计划“卢嘉锡国际团队项目”（GJTD-2020-12）；广东省重点领域研发计划项目（2020B1111030005）；广东省科学技术厅项目（2019TQ05H299）

Study on the secondary metabolites from the South China Sea derived fungus Penicillium sp. SCSIO 40438

ZHU Yiguang1, 2, 3, MOU Pengyun1, 4, ZHANG Qingbo1, 2, 3, ZHANG Changsheng1, 2, 3   

  1. 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

    2. University of Chinese Academy of Sciences, Beijing 100049, China

    3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, Guangdong, China

    4. College of Life Science and Technology, Tarim University, Alar 843300, China

  • Received:2022-04-14 Revised:2022-05-08 Online:2022-05-10 Published:2022-05-10
  • Contact: Qingbo Zhang
  • Supported by:

    Guangdong MEPP Funds (GDNRC[2021]48); K.C.Wong Education Foundation (GJTD-2020-12); Key-Area Research and Development Program of Guangdong Province (2020B1111030005); Special Support Program for Training High-Level Talents in Guangdong (2019TQ05H299)

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摘要： 本论文对中国南海来源真菌Penicillium sp. SCSIO 40438的次级代谢产物进行研究。通过硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及高效液相色谱等多种分离方法对该菌株的发酵产物进行分离纯化，利用核磁共振和高分辨质谱等波谱学方法对分离得到的化合物进行结构鉴定。从中共分离鉴定到9个化合物：1-(2-Methylbut-3-en-2-yl)-1H-indole-3-carbaldehyde (1)、1-methyl-2(1H)-quinazolinone (2)、fructigenine A (3)、fructigenine B (4)、2-[(s)-hydroxy(phenyl)methyl]-3-methylquinazolin-4(3H)-one (5)、3-methylviridicatin (6)、3-O-methylviridicatol (7)、viridicatol (8)和(+)-cyclopenol (9)，其中化合物1和2为新天然产物。抑菌活性实验表明，化合物8具有中等强度的抑制金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌活性，最低抑菌浓度为8.0 μg mL-1。

关键词: 真菌, Penicillium sp., 次级代谢产物, 抑菌活性

Abstract: This experiment aims to study the secondary metabolites from the South China Sea derived fungus Penicillium sp. SCSIO 40438. The solid fermentation products of the strain were isolated and purified by a variety of separation methods, including silica gel column, Sephadex LH-20 gel column, and preparative high performance liquid chromatography. The structures of the isolated compounds were identified by nuclear magnetic resonance, high resolution electrospray ionization mass spectroscopy and comparison with the reported data. Nine compounds were obtained and identified as 1-(2-Methylbut-3-en-2-yl)-1H-indole-3-carbaldehyde (1)、1-methyl-2(1H)-quinazolinone (2), fructigenine A (3), fructigenine B (4), 2-[(s)-hydroxy(phenyl)methyl]-3-methylquinazolin-4(3H)-one (5), 3-Methylviridicatin (6), 3-O-methylviridicatol (7), viridicatol (8), (+)-cyclopenol (9), and compounds 1 and 2 are two new natural products. Compound 8 showed moderate antibacterial activities against Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus with minimal inhibition concentration (MIC) values of 8.0 μg mL-1.

Key words: fungi, Penicillium sp., secondary metabolites, antibacterial activity