热带海洋学报

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海南澳洲管体星虫线粒体基因组特征及进化分析

黄培贤1, 2, 姚雪梅1, 2, 余巧驰1, 2, 张佳玉1, 2   

  1. 1. 南海海洋资源利用国家重点实验室(海南大学), 海南 海口 570228;

    2. 海南大学海洋学院, 海南 海口 570228

  • 收稿日期:2022-04-19 修回日期:2022-07-22 出版日期:2022-07-27 发布日期:2022-07-27
  • 通讯作者: 姚雪梅
  • 基金资助:

    海南省自然科学基金(319MS013)

Mitogenome characteristics and phylogenetic analysis of Siphonosoma australe in Hainan

HUANG Peixian1, 2, YAO Xuemei1, 2, YU Qiaochi1, 2, ZHANG Jiayu1, 2   

  1. 1. State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China;

    2. College of Ocean, Hainan University, Haikou 570228, China

  • Received:2022-04-19 Revised:2022-07-22 Online:2022-07-27 Published:2022-07-27
  • Contact: Xue-Mei YAO
  • Supported by:

    Hainan Provincial Natural Science Foundation of China (319MS013)

摘要: 澳洲管体星虫是海南当地的特色经济价值高的海产资源。澳洲管体星虫隶属管体星虫属,该属在星虫动物门内的分类地位一直极具争议。本研究通过高通量测序测定海南文昌地区澳洲管体星虫的线粒体基因组,利用Clustal、DnaSP、Mega、MrBayes等软件对其基因序列特征、遗传变异、系统进化进行分析,并重新进一步探讨该属在星虫动物门内的进化地位。结果显示,管体星虫属澳洲管体星虫线粒体基因组由37个基因组成,其中包括12个编码蛋白基因、23个tRNA和2个rRNA。澳洲管体星虫的线粒体基因组基因排列高度保守,澳洲管体星虫线粒体基因组编码蛋白基因在COX2与COX3之间无ATP8。在主编码基因中,ND2、ND4、ND5的变异位点数量较多,比例较高,在星虫动物种水平的种间或种内多样性及进化分析研究中适合用作分子标记;CYTB、COX1、COX2的变异位点数量较少,适用于种或属水平的进化分析研究。与来自Genbank中3个属6个种的星虫线粒体基因组构建的进化树(NJ、ML、BI)显示,管体星虫属介于革囊星虫属和方格星虫属之间,与革囊星虫属关系较近,反而与方格星虫属的关系较远,与传统的形态学分类不一致。形态学上,管体星虫属混合了革囊星虫属和方格星虫属的形态特征,而线粒体基因组构建的进化树更能体现管体星虫属真实且独特的进化地位。线粒体基因组分子数据为保护海南本地渔业资源的多样性及解释管体星虫属的进化地位提供了理论依据。

关键词: 澳洲管体星虫, 海南, 线粒体基因组, 系统进化

Abstract: Siphonosoma australe is a local economic fishery resource of Hainan. S. australe belongs to Siphonosoma, and the taxonomic status of the genus in the phylum Sipuncula has been highly controversial. In this study, the mitogenome of S. australe in the Wenchang coast of Hainan was determined by high-throughput sequencing. Characteristics, genetic variation & phylogenetic evolution of mitogenome sequence were analyzed by using Clustal、DnaSP、Mega、MrBayes, further more were used to explore the evolutionary position of the genus Siphonosoma in the phylum of Sipuncula. The results show that the mitogenome has 16483 base pairs and made up of a total of 37 genes (12 protein-coding, 23 transfer RNAs, and 2 ribosomal RNAs). The mitogenome gene arrangement of S. australe is highly conservative, but there is no ATP8 gene between genetic sites of COX2 & COX3. Among the main coding genes (ND2, ND4 and ND5) have a large number of variation sites, which are suitable to be used as molecular markers in the study of inter- & intraspecific diversities and phylogenetic analyses, while the number of variation sites of CYTB, COX1 and COX2 is relatively small, which is suitable for species-level or genus-level phylogeny. The phylogenetic tree (NJ、ML and BI) constructed by the mitogenome of sipunculans from 3 genera and 6 species in GenBank showed that the genus Siphonosoma as an independent clade is between the genera of Phascolosoma and Sipunculus , even close to the genus of Phascolosoma instead far from the genus of Sipunculus. This result is inconsistent with the previously traditional classification based on morphological analyses. Taxonomically, Siphonosoma mixes the morphological characteristics of Phascolosoma and Sipunculus. The phylogenetic tree constructed by mitochondrial genome could better reflect unique phylogenetic status of Siphonosoma. mitogenomic data of S. australe provides theoretical basis for the protection of the fishery-source diversity, and give proof to the interpretation to the newly phylogenetic position of Siphonosoma.

Key words: Siphonosoma australe, Hainan, mitogenome, phylogeny

中图分类号: 

  • S917