热带海洋学报

• •    

深海放线菌Streptomyces koyangensis SCSIO 5802中次级代谢产物neoabyssomicin H的分离鉴定

宋永相1, 2, 3, 4*,李晓悦1, 3,鞠建华1, 2, 3, 4   

  1. 1. 中国科学院南海海洋研究所 中国科学院热带海洋生物资源与生态重点实验室,广东省海洋药物重点实验室,广州 510301

    2. 南方海洋科学与工程广东省实验室(广州),广州市南沙区南沙街资讯科技园海滨路1119 号,广州 511458

    3. 中国科学院大学,北京 100049

    4. 三亚中科海洋研究院,崖州湾深海科技城,三亚,572000

  • 收稿日期:2022-05-13 修回日期:2022-06-13 出版日期:2022-06-16 发布日期:2022-06-16
  • 通讯作者: 宋永相
  • 基金资助:
    海南省重大科技资助计划(ZDKJ202018);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0406);广东省本土创新创业团队项目(2019BT02Y262);广东省海洋经济发展(海洋六大产业)专项资金GDNRC[2021]54;国家自然科学基金面上项目(41676151)

The isolation and identification of neoabyssomicin H from the deep-sea derived Streptomyces koyangensis SCSIO 5802

SONG Yongxiang1,2,3,4*, LI Xiaoyue1,3, JU Jianhua1,2,3,4   

  1. 1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China;

    2. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou),No.1119, Haibin Rd., Nansha District, Guangzhou 511453;

    3. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China;

    4. Sanya Institute of Oceanology, SCSIO, Yazhou Scientific Bay, Sanya, 572000, China

  • Received:2022-05-13 Revised:2022-06-13 Online:2022-06-16 Published:2022-06-16
  • Contact: Yongxiang Song
  • Supported by:
     Finance Science and Technology Project of Hainan Province (ZDKJ202018); Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0406); Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (2019BT02Y262); the GDNRC[2021]54 and National Natural Science Foundation of China (41676151)

摘要: 深渊霉素类化合物具有抗结核分枝杆菌、抗MRSA (methicillin-resistant Staphylococcus aureus)、选择性激活潜伏HIV病毒等生物活性,具有显著的开发利用潜力。本文在前期研究基础上,进一步对深海链霉菌Streptomyces koyangensis SCSIO 5802新型深渊霉素类化合物的生产潜力进行发掘,综合利用多种色谱分离手段,获得一个结构新颖的深渊霉素硫醚二聚体化合物,命名为neoabyssomicin H。该化合物的结构通过UV、IR、HR-ESI-MS,1D和2D NMR及X-Ray单晶铜靶衍射等进行了鉴定。活性测试结果表明,neoabyssomicin H对金黄色葡萄球菌和系列临床MRSA的MIC(minimum inhibitory concentration)值大于128μg·mL-1。该研究进一步丰富了深渊霉素类化合物,为硫醚二聚体类深渊霉素活性化合物的研究提供了新的分子实体。

关键词: 深海放线菌, 深渊霉素, 抗菌, 硫醚二聚体

Abstract: Abyssomicins have attracted much attention for their potential of being developed into novel lead compounds by virtue of their antimicrobial and antiviral activities. The deep-sea derived strain of Streptomyces koyangensis SCSIO 5802 produces a series of abyssomicins. In order to further expand the library of abyssomicins, the fermentation fractions of this strain have been thoroughly investigated, which led to first isolation of a new thioether dimer of abyssomicin with a macrolide moiety named neoabyssomicin H. Its structure was elucidated by using UV, IR, HR-ESI-MS, 1D and 2D NMR, and X-ray single crystal using Cu Kα radiation, etc. In antibacterial activity assays, the compound was found inactive against staphylococcus aureus ATCC 29213 and a panel of MRSA clinical isolates at 128μg·mL-1. This study has expanded the scope of abyssomicins with a new molecular entity for research on this class of lead compounds.

Key words: Deep-sea derived actinomycete, abyssomicin, antibacteria, thioether dimerization