热带海洋学报 ›› 2010, Vol. 29 ›› Issue (3): 61-64.doi: 10.11978/j.issn.1009-5470.2010.03.061

• 海洋生物学 • 上一篇    下一篇

不同培养基对小球藻Chlorella zofingiensis生长和虾青素产量的影响

彭娟1,2, 王艳1, 向文洲1, 陈峰1,3   

  1. 1. 中国科学院南海海洋研究所, 广东 广州  510301; 2. 中山大学海洋学院, 广东 广州 510275; 3. 香港大学植物学系, 香港
  • 收稿日期:2008-01-07 修回日期:2008-04-08 出版日期:2010-07-01 发布日期:2010-05-24
  • 作者简介:彭娟(1980—), 女, 湖北省松滋县人, 博士, 主要从事微藻生物技术研究。
  • 基金资助:

    中国科学院 “百人计划”课题资助

Effects of different culture media on the growth and production of astaxanthin by Chlorella zofingiensis

PENG Juan1, WANG Yan1, XIANG Wen-zhou1, CHEN Feng1,2   

  1. 1. South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, 510301; 2. Department of Botany, the Uni-versity of Hong Kong, Hong Kong, P. R. China
  • Received:2008-01-07 Revised:2008-04-08 Online:2010-07-01 Published:2010-05-24
  • About author:彭娟(1980—), 女, 湖北省松滋县人, 博士, 主要从事微藻生物技术研究。
  • Supported by:

    中国科学院 “百人计划”课题资助

摘要:

研究了小球藻Chlorella zofingiensis在3种不同培养基CZ-M1, Kuhl 和 KM1中培养时生物量和虾青素的产量。结果表明, Kuhl最利于小球藻的生长, 比生长速率、最大细胞干重和得率最高, 虾青素含量最低; KM1培养基最利于虾青素的积累。采用优化后的培养基KM1, 添加葡萄糖诱导培养小球藻, 可以获得8.99g•L-1的藻细胞干重, 虾青素产量和含量分别达到20.1mg•L-1和2.24mg•g-1。

关键词: 小球藻, 虾青素, 培养基

Abstract:

The biomass and astaxanthin yield of Chlorella zofingiensis cultivated in three different media including CZ-M1, Kuhl and KM1 were investigated. Results showed that Kuhl medium was best for growth as reflected by its highest values of specific growth rate, maximum cell dry weight concentration and cell growth yield on glucose comparing with the other media. However, its astaxanthin production was the lowest. KM1 exhibited the highest astaxanthin production both on a per dry cell weight and a per volume basis. Higher biomass (up to 8.99g•L-1 cell dry weight concentration) and astaxanthin levels (up to 20.1mg•L-1 and 2.24mg•g-1) in glucose-stressed Chlorella zofingiensis cells growing under low light in modified KM1 medium were obtained.

Key words: Chlorella zofingiensis, astaxanthin, media