热带海洋学报 ›› 2010, Vol. 29 ›› Issue (3): 65-70.doi: 10.11978/j.issn.1009-5470.2010.03.065

• 海洋生物学 • 上一篇    下一篇

NaHSO3对盐生杜氏藻生长和光合色素含量的影响

程建峰1,2, 胡芬红1, 沈允钢1   

  1. 1. 中国科学院上海生命科学研究院植物生理生态研究所, 上海 200032; 2. 江西农业大学农学院, 江西 南昌 330045
  • 收稿日期:2008-01-25 修回日期:2008-07-02 出版日期:2010-07-01 发布日期:2010-05-24
  • 通讯作者: 沈允钢(1927—), 男, 研究员, 中国科学院院士, 主要从事光合作用研究。
  • 作者简介:程建峰(1972—), 男, 江西省横峰县人, 博士后, 副教授, 主要从事植物生理生态研究。E-mail: chjfkarl@163.com
  • 基金资助:

    中国科学院创新基金(101CK0104)

Effects of NaHSO3 on the growth and contents of photosynthetic pigments in Dunaliella salina

CHENG Jian-feng1,2, HU Fen-hong1, SHEN Yun-gang1   

  1. 1. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, China Academy of Sciences, Shanghai 200032, China; 2. College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2008-01-25 Revised:2008-07-02 Online:2010-07-01 Published:2010-05-24
  • Contact: 沈允钢(1927—), 男, 研究员, 中国科学院院士, 主要从事光合作用研究。
  • About author:程建峰(1972—), 男, 江西省横峰县人, 博士后, 副教授, 主要从事植物生理生态研究。E-mail: chjfkarl@163.com
  • Supported by:

    中国科学院创新基金(101CK0104)

摘要:

为优化盐生杜氏藻营养盐配方、降低养殖成本、提高生物量及色素积累, 研究了不同浓度的安全环保的植物循环光合磷酸化促进剂——NaHSO3(≤0.40mM•L-1)溶液对盐生杜氏藻生长和光合色素含量的影响。结果表明, NaHSO3可显著促进盐生杜氏藻生长, 提高叶绿素a (Chl a)和b(Ch1 b)含量、叶绿素总量(Chl)、类胡萝卜素含量(Car)和Chl/Car; 促进效应随浓度增加表现为先升后降, 低浓度(<0.10mM•L-1)效应较好, 以0.07mM•L-1为最佳。NaHSO3提高Chl b的效应大于Chl a, 降低Chl a/Chl b比值, 随浓度增加表现为先降后升, 以0.07mM•L-1降低最大。本试验条件下, 盐生杜氏藻生物量与光合色素含量间及各光合色素含量间呈显著或极显著相关, 与生物量(Y, g•L-1)相关最大的是Chl b (XChl b, μg•ml-1), 符合Y=0.284XChl b-0.883 (R2=0.994**)(**表示Y与X之间在0.01水平上极显著相关,下同); 其次为Chl (XChl, μg•ml-1), Y=0.100XChl-2.022 (R2=0.993**), 为生长动态的及时掌握提供了有效的间接衡量指标。

关键词:  盐生杜氏藻Dunaliella salina, NaHSO3, 生长, 生物量, 光合色素

Abstract:

Bisulfite (NaHSO3) is a substance to accelerate plant cyclic photophosphorylation that can be used safely and environmentally friendly. In order to optimize the nutrients formula, decrease the production cost and improve the biomass and pigment accumulation of Dunaliella salina, the authors investigated the effects of different concentrations (0.00?0.40mM•L-1) of NaHSO3 on the biomass and photosynthetic pigment contents. The results showed that NaHSO3 could significantly improve the growth, contents of chlorophyll (Chl) a, Chl b, Chl (a+b), carotenoid (Car) and ratio of Chl to Car. The effects firstly increased and then decreased with the increase of concentration. Accelerating effects under low concentrations (<0.10mM•L-1) were better than those under high concentrations (0.10?0.40mM•L-1), and the maximum accelerating effects were reaches at 0.07mM•L-1. NaHSO3 could increase Chl b content more than Chl a content, which caused the reduction of Chl a content relative to Chl b content; the effects firstly decreased and then increased with the increase of concentration, reducing effects under low concentrations (<0.1mM•L-1) were better than those under high concentrations (0.10?0.40mM•L-1), and the minimum reducing effect was achieved at 0.07mM•L-1. The correlations of biomass (Y) to contents of photosynthetic pigments and between contents of photosynthetic pigments were significant or markedly significant, with the highest correlation of Chl b (XChl.b) to biomass (Y=0.284XChl.b-0.883 (R2=0.994**)) and the next of Chl to biomass (Y=0.100XChl-2.022 (R2=0.993**)), which provided an indirect index to measure the growth of Dunaliella salina in every moment.

Key words:  Dunaliella salina, NaHSO3, growth, biomass, photosynthetic pigment