热带海洋学报 ›› 2010, Vol. 29 ›› Issue (3): 82-86.doi: 10.11978/j.issn.1009-5470.2010.03.082

• 海洋生物学 • 上一篇    下一篇

9种常见笛鲷微卫星位点筛选与遗传多样性分析

郭昱嵩1,2, 王中铎1,2, 刘楚吾1, 陈志明1, 刘筠2   

  1. 1. 广东海洋大学水产学院, 广东 湛江 524025; 2. 湖南师范大学生命科学院, 湖南 长沙 410081
  • 收稿日期:2008-03-25 修回日期:2008-07-15 出版日期:2010-07-01 发布日期:2010-05-24
  • 通讯作者: 刘楚吾
  • 作者简介:郭昱嵩(1980—), 女, 湖南省桂东县人,博士, 讲师, 从事海洋生物分子遗传学研究。E-mail: gysrabbit@163.com
  • 基金资助:

    国家自然科学基金资助项目(30671610), 国家科技支撑计划项目(2007BAD29B03)

Isolation and genetic diversity analysis of microsatellites from nine species of familiar snappers

GUO Yu-song1,2, WANG Zhong-duo1,2, LIU Chu-wu1, CHEN Zhi-ming1, LIU Yun2   

  1. 1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China; 2. College of Life Science, Hunan Normal University, Changsha 410081, China
  • Received:2008-03-25 Revised:2008-07-15 Online:2010-07-01 Published:2010-05-24
  • Contact: 刘楚吾
  • About author:郭昱嵩(1980—), 女, 湖南省桂东县人,博士, 讲师, 从事海洋生物分子遗传学研究。E-mail: gysrabbit@163.com
  • Supported by:

    国家自然科学基金资助项目(30671610), 国家科技支撑计划项目(2007BAD29B03)

摘要:

 利用从勒氏笛鲷Lutjanus russelli基因组文库中筛选得到37对微卫星引物对笛鲷属9个习见种红鳍笛鲷   L. erythropterus、紫红笛鲷L. argentimaculatus、星点笛鲷L. stellatus、约氏笛鲷L. johnii、千年笛鲷L. sebae、金带笛鲷L. fulvus、金焰笛鲷L. fulviflamma、画眉笛鲷L. vitta与奥氏笛鲷L. ophuysenii的微卫星位点进行筛选和分析。9个种都能检测到的微卫星位点有10个, 部分种能检测到的有13个。9种笛鲷Hardy–Weinberg平衡下的平均期望杂合度在0.730―1.000, 平均观测杂合度在0.716―0.915, 偏离指数(D)为-0.002― -0.214。微卫星位点杂合度的分析表明目前笛鲷属鱼类存在较高的遗传多样性水平。另发现9个种的笛鲷都出现了杂合子缺失的情况, 缺失的原因有待于进一步的研究予以解释。

关键词: 笛鲷属(Lutjanus), 微卫星DNA, 遗传多样性

Abstract:

Thirty-seven microsatellite loci were isolated from the genomic library of L. russellii with PCR. Cross-species isolations with those markers were carried out for nine familiar species of snappers (L. erythropterus, L. argentimaculatus, L. stellatus, L. johnii, L. sebae, L. fulvus, L. fulviflamma, L. vitta and L. ophuysenii), and their genetic diversities were also evaluated. There were 10 microsatellite loci isolated successfully in nine species and 13 loci in partial species. The average expected heterozygosity, average observed heterozygosity and Hardy–Weinberg departure index under Hardy–Weinberg equilibrium in the nine species of snappers were 0.730?1.000, 0.716?0.915, (-0.002) -(-0.214), respectively. The results indicate that the genetic diversities of genus Lutjanus is in a relatively high level. In addition, the clear explain of prevalent absence of heterozygotes in all research species may request further research on fishes of genus Lutjanus.