热带海洋学报 ›› 2010, Vol. 29 ›› Issue (3): 120-125.doi: 10.11978/j.issn.1009-5470.2010.03.120

• 海洋生物学 • 上一篇    下一篇

饥饿对方斑东风螺幼螺生化组成和消化酶活力的影响

薛明1, 2, 柯才焕1, 魏永杰1   

  1. 1. 厦门大学海洋学系, 厦门大学近海海洋环境科学国家重点实验室, 福建 厦门 361005; 2. 广东海洋大学水产学院, 广东 湛江 524025
  • 收稿日期:2009-07-31 修回日期:2009-11-29 出版日期:2010-07-01 发布日期:2010-05-24
  • 通讯作者: 柯才焕
  • 作者简介:薛明(1975—), 女, 安徽省桐城市人, 讲师, 硕士, 主要从事海洋无脊椎动物学研究。
  • 基金资助:

    现代农业产业技术体系建设专项资金(nycytx-47); 科技部农业科技成果转化(03EFN213500133)

Effects of starvation on biochemical compositions and digestive enzyme activities of spotted babylon, Babylonia areolata juveniles

XUE Ming1,2, KE Cai-huan1, WEI Yong-jie1   

  1. 1. Department of Oceanography, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China; 2. Fisheries College of Guangdong Ocean University, Zhanjiang 524025, China
  • Received:2009-07-31 Revised:2009-11-29 Online:2010-07-01 Published:2010-05-24
  • Contact: 柯才焕
  • About author:薛明(1975—), 女, 安徽省桐城市人, 讲师, 硕士, 主要从事海洋无脊椎动物学研究。
  • Supported by:

    现代农业产业技术体系建设专项资金(nycytx-47); 科技部农业科技成果转化(03EFN213500133)

摘要:

通过室内试验测定方斑东风螺Babylonia areolata在饥饿120d过程中体内生化组成、能值及消化系统主要消化酶活力的连续变化。结果表明, 随着饥饿时间延长, 螺体的水分与灰分含量逐渐上升, 而蛋白质、脂肪及糖原含量和能值均呈下降趋势。胃、肠道与肝胰脏中脂肪酶总活力在饥饿10d时较对照组略有上升, 随后呈下降趋势; 淀粉酶总活力和蛋白酶总活力均随饥饿时间延长而降低。饥饿70d时, 脂肪酶、淀粉酶、蛋白酶分别降为对照的70.12%、63.66%、42.79%, 脂肪酶与淀粉酶总活力在70—90d时迅速下降; 各酶活力在90—120d均降至较低的水平。结果进一步显示, 幼螺在饥饿前期主要消耗脂肪与糖原供能, 70d后加大对贮备蛋白质的动用量, 而后期   (90—120d)则以利用蛋白质为主; 各消化酶活性在饥饿90d前快速下降, 之后呈恒定状态。饥饿时螺体含水量可作为预测其营养状态的指标。

关键词: 方斑东风螺Babylonia areolata, 饥饿, 生化组成, 能值, 消化酶活力

Abstract:

An experiment was conducted to determine the effects of starvation on biochemical compositions, energy values and activities of protease, lipase and amylase of the digestive system of Babylonia areolata juveniles during a period of 120 days. The results indicated that water and ash contents in the whole soft body increased gradually, while the contents of protein, lipid and glycogen, as well as the energy values, decreased greatly during starvation. The total lipase activity obtained from stomach, intestine and hepatopancreas was higher than that from the control when the snails were deprived for 10 days; it decreased markedly during starvation, and the total activities of amylase and protease reduced gradually with prolonged starvation period. The relative activity percentages were 70.12% and 63.66% for lipase and amylase when compared to the control after being starved for 70 days, whereas the responding value was 42.79% for protease. The activities of lipase and amylase showed a marked decrease from the 70th to the 90th day; they remained stable relatively thereafter. In conclusion, the lipid and glycogen contents were mobilized greatly during early growth period, while the protein was metabolized when the snails were starved longer than 70 days and served as the predominant energy source 90 days later. The total digestive enzyme activities of all three kinds maintained at lower level from the 90th to 120th day after a rapid decrease, and the water content could be used as an effective marker to estimate the nutrient status of the snails during starvation period.

Key words: Babylonia areolata, starvation, biochemical compositions, energy value, digestive enzyme activities