热带海洋学报 ›› 2010, Vol. 29 ›› Issue (6): 147-152.doi: 10.11978/j.issn.1009-5470.2010.06.147

• 海洋生物学 • 上一篇    下一篇

海洋蛭弧菌类生物DA5对凡纳滨对虾育苗期幼体和水质的影响

温崇庆1, 2, 3, 梁华芳1, 丁贤2, 3, 薛明1, 周世宁3   

  1. 1. 广东海洋大学水产学院, 广东 湛江524025; 2. 中国水产科学研究院南海水产研究所/农业部渔业生态环境重点开放实验室, 广东 广州510300; 3. 中山大学生命科学学院//有害生物控制与资源利用国家重点实验室, 广东 广州510275
  • 收稿日期:2009-01-08 修回日期:2009-06-22 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 周世宁。
  • 作者简介:温崇庆(1974―), 男, 安徽省郎溪县人, 副教授, 博士, 主要从事海洋和水产微生物学研究。E-mail: wencq@gdou.edu.cn
  • 基金资助:

    国家“863”项目(2007AA09Z448); 农业部渔业生态环境重点开放实验室开放基金;广东省渔业生态环境重点实验室开放基金项目(2006-8); 广东省海洋渔业科技推广专项项目(A200808A04) ; 广东海洋大学自然科学基金项目(0812067)

Effects of marine Bdellovibrio-and-like organism DA5 on larval survival and water quality in larval rearing of Litopenaeus vannamei

WEN Chong-qing1,2,3, LIANG Hua-fang1, DING Xian2,3, XUE Ming1, ZHOU Shi-ning3   

  1. 1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China; 2. Key Laboratory of Fishery Ecology Environment of Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 3. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
  • Received:2009-01-08 Revised:2009-06-22 Online:2010-12-15 Published:2010-12-15
  • Contact: 周世宁。
  • About author:温崇庆(1974―), 男, 安徽省郎溪县人, 副教授, 博士, 主要从事海洋和水产微生物学研究。E-mail: wencq@gdou.edu.cn
  • Supported by:

    国家“863”项目(2007AA09Z448); 农业部渔业生态环境重点开放实验室开放基金;广东省渔业生态环境重点实验室开放基金项目(2006-8); 广东省海洋渔业科技推广专项项目(A200808A04) ; 广东海洋大学自然科学基金项目(0812067)

摘要:

以热灭活的溶藻弧菌Vibrio alginolyticus培养海洋蛭弧菌类生物菌株DA5, 将其以高(11500pfu•mL?1)、低(1150pfu•mL?1)两种浓度加入凡纳滨对虾Litopenaeus vannamei育苗用水中, 研究了无节幼体培育到糠虾幼体期间DA5对幼体变态和存活、水体中异养细菌和弧菌含量、pH、化学需氧量(COD)和氨氮(NH3-N)含量的影响。结果显示, 高浓度DA5可显著提高幼体存活和变态率, 并在加入后3d内降低或显著降低水体异养细菌和弧菌含量, 而低浓度DA5对试验期间幼体成活、变态及水体异养细菌和弧菌含量均无显著影响。除糠虾幼体Ⅰ—Ⅱ期高浓度处理组水体NH3-N含量有显著升高外, 试验期间各组水体pH、COD和NH3-N含量均无显著差异。表明海洋蛭弧菌类生物DA5可作为一种较理想的生物控制因子应用于对虾育苗中。

关键词: 海洋蛭弧菌类生物, 凡纳滨对虾Litopenaeus vannamei, 幼体, 水质

Abstract:

The authors studied the effects of marine Bdellovibrio-and-like organisms (BALOs) strain DA5, which was cultured from heat-killed Vibrio alginolyticus, on larval survival and water quality from nauplius stage to mysis stage of Litopenaeus vannamei. The results showed that the survival rate and metamorphic rate of mysis larvae treated with high concentration of DA5 (11500pfu•mL?1; HC) could be improved visibly, and the amounts of heterotrophs and vibrios in rearing water were re-duced or significantly reduced in the first 3 days. However, there were no obvious effects on larval survival and bacterial amount treated with low concentration of DA5 (1150pfu•mL?1; LC) during the larval rearing period. There were no significant differences in pH, chemical oxygen demand, and ammonia-N content in water body among the HC, LC, and control groups, except that the ammonia-N content in the HC group at mysis Ⅰ-Ⅱ stage was increased significantly. This study indicated that marine BALOs strain DA5 can be applied in larval rearing of shrimp as a promising biocontrol agent.

Key words: marine Bdellovibrio-and-like organism, Litopenaeus vannamei, larva, water quality