热带海洋学报 ›› 2012, Vol. 31 ›› Issue (5): 124-130.doi: 10.11978/j.issn.1009-5470.2012.05.018

• 海洋生物学 • 上一篇    

饥饿胁迫对九孔鲍免疫防御因子的影响*

陈政强1, 2, 3, 陈昌生1, 2, 战文斌3, 林茂1, 2, 杨红玲1, 2   

  1. 1. 集美大学水产学院, 福建 厦门361021; 2. 福建省高校水产科学技术与食品安全重点实验室, 福建 厦门361021; 3. 中国海洋大学水产学院, 山东 青岛266003
  • 收稿日期:2011-09-12 修回日期:2012-03-03 出版日期:2012-11-01 发布日期:2013-02-06
  • 通讯作者: 战文斌 (1960—), 男, 教授, 博士, 从事水产病害与免疫研究。E-mail: wbzhan@ouc.edu.cn。实验用九孔鲍。
  • 作者简介:陈政强(1967—), 男, 副教授, 博士, 从事海洋水产研究。
  • 基金资助:
    福建省自然科学基金资助项目(2006J0420)

Effect of starvation stress on immune response of abalone Haliotis diversicolor supertexta and anti-infectious resistance against Vibrio parahaemolyticus

CHEN Zheng-qiang1, 2, 3, CHEN Chang-sheng1, 2 , ZHAN Wen-bin 3, LIN Mao1, 2, YANG Hong-ling1, 2   

  1. 1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Key Laboratory of Science and Technology for Aquaculture and Food Safety (Jimei University), Fujian province, Xiamen 361021, China; 3. Fisheries College of Ocean University of China, Tsingtao 266003, China
  • Received:2011-09-12 Revised:2012-03-03 Online:2012-11-01 Published:2013-02-06

摘要: 平均体重为14.25±2.21g的九孔鲍在水温24.3°C±1.7°C、盐度为(30.42±1.63)‰、pH值为8.15±0.11的环境条件下, 饥饿胁迫20d是九孔鲍生理上可以承受的。九孔鲍在停食后5d时间内, 血淋巴液蛋白含量没有显著变化,而7d后, 九孔鲍血淋巴液蛋白质含量逐渐大幅度下降, 由此推测, 九孔鲍在饥饿7d后体蛋白被大量消耗于机体的生命维持。饥饿胁迫状态下九孔鲍血淋巴细胞吞噬活性下降, 血淋巴细胞呼吸爆发水平下降, 体液溶菌酶活性、凝集活性下降, 抗感染免疫机能下降; 但是, 九孔鲍经历5d时间的饥饿, 血淋巴抗菌清除效率、血淋巴细胞吞噬活性、血淋巴细胞呼吸爆发产生的超氧阴离子水平、体液溶菌酶活性等免疫指标都变化不大, 说明九孔鲍具有面对饥饿胁迫的应激适应机制。饥饿之初, 机体通过维持既有免疫机能来适应环境、保护自我; 饥饿胁迫持续时间超过7d, 这种本能的逆境适应方式和机能维持终因营养不济和体内资源的持续消耗而改变和衰退。饥饿胁迫下的九孔鲍血淋巴液中的溶菌酶活性和凝集活性下降可能直接由体液蛋白含量下降引起, 而胞内颗粒性物质减少则可能是血淋巴液中溶菌酶及凝集素活性减弱的直接原因。

关键词: 九孔鲍, 饥饿胁迫, 免疫应答, 抗感染力, 副溶血弧菌

Abstract: The abalones of H. diversicolor supertexta with an average body weight of 14.25?2.21g can survive over a starvation period of 20 days at 24.3±1.7°C water temperature, (30.42?1.63)‰ salinity, and pH of 8.15?0.11. The concentration of haemolymph protein in the abalones varied slightly in five days when exposed to starvation and substantially dropped progressively after seven days of starvation. Based on these resutls, it is surmised that there was mass humoral protein consumed for life support from 7d to 20d during starvation. The abalones survived with following decreases: a) phagocytosis activity of haemocytes; b) respiratory burst; c) haemolymph lysozyme activity; d) haemolymph agglutination titers and e) anti-infectious immunity during starvation; however, some immune parameters, such as haemolymph clearance efficiency , phagocytosis activity of haemocytes , respiratory burst and haemolymph lysozyme activity, sometimes went up somewhat in the first five days of starvation. Consequently, it is suggested that the abalones are born with functions of starvation stress adaptation. In early period of starvation the abalones did protect themselves and adaptate to surroundings by the way of improving immune effection owing to subnutrition, and this biophilia styles of stress adaptation and natural ability maintaining changed or degenerated from day seven of starvation resulted from seriously poor nutritional state?and excessively?intravital resources degradation. Perhaps the haemolymph lysozyme activity and agglutinating titers decreased resulting from reduction of haemolymph protein, while the intracellular granules reduction may directly result in haemolymph lysozyme activity and agglutinating titers dropped.

Key words: Haliotis diversicolor supertexta, starvation stress, immune response, resistance, Vibrio parahaemolyticus

中图分类号: 

  • P735.54