热带海洋学报 ›› 2018, Vol. 37 ›› Issue (1): 1-11.doi: 10.11978/2017041

• •    下一篇

褐斜鲽(Plagiopsetta glossa)线粒体基因组特征及重排机制研究

董江星1,2(), 时伟1, 孔晓瑜1(), 陈世喜1,2   

  1. 1. 中国科学院热带海洋生物资源与生态重点实验室(中国科学院南海海洋研究所), 广东 广州 510301
    2. 中国科学院大学, 北京 100049
  • 收稿日期:2017-04-11 修回日期:2017-05-25 出版日期:2018-01-20 发布日期:2018-02-02
  • 作者简介:

    作者简介:董江星(1989—), 男, 河南省鹤壁市人, 硕士, 从事鱼类分类及系统进化研究。E-mail: djx571@yeah.net

  • 基金资助:
    国家自然科学基金项目(31471979、30870283)

Characteristic analysis of the complete mitogenome of Plagiopsetta glossa and a possible mechanism for gene rearrangement

Jiangxing DONG1,2(), Wei SHI1, Xiaoyu KONG1(), Shixi CHEN1,2   

  1. 1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Guangzhou 510301, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2017-04-11 Revised:2017-05-25 Online:2018-01-20 Published:2018-02-02
  • Supported by:
    National Natural Science Foundation of China (31471979, 30870283)

摘要:

冠鲽科(Samaridae)隶属于鲽形目(Pleuronectiformes), 包含冠鲽属(Samaris)、沙鲽属(Samariscus)和斜鲽属(Plagiopsetta)。目前研究表明, 冠鲽(Samaris cristatus)和满月沙鲽(Samariscus latus)的线粒体基因组结构都有重排, 并且两者的基因数量也有差别。为检测斜鲽属鱼类中是否也有不同的特征结构, 我们选用褐斜鲽(Plagiopsetta glossa)作为代表种进行斜鲽属鱼类线粒体基因组特征分析, 同时与冠鲽属及沙鲽属鱼类的线粒体基因组特征进行对比。分析显示, 褐斜鲽的线粒体基因组全长为18723bp, 包括39个基因: 13个蛋白基因、24个tRNA基因、2个rRNA基因、2个控制区、1个轻链复制起点和比典型基因组多的13个间隔子。和经典鱼类的线粒体基因组相比, 褐斜鲽和满月沙鲽都多了tRNA-CystRNA-Tyr两个tRNA, 冠鲽只多了tRNA-Cys, 且3个种类都多了一个控制区, 但褐斜鲽与满月沙鲽的基因排列顺序相同。褐斜鲽线粒体基因的重排导致不同位置的6个tRNA形成了六连体基因簇“tRNA-Cys1-Tyr1- Ser1-Lys-Arg-Ser2”, “ND5-ND6-Glu-Cytb-Thr”则位于六连体之后, 但这11个基因相对的排序没有发生变化。采用双复制随机丢失模型(double replications and random loss, DRRL)对褐斜鲽基因的重排现象进行分析, 认为该鱼类基因数量、排列顺序以及比典型基因组多13个间隔子等特征为该模型提供了新的依据。

关键词: 鲽形目, 冠鲽科, 线粒体基因组, 基因重排, 分子进化

Abstract:

The family Samaridae in Pleuronectiformes consists of three genera, namely, Plagiopsetta, Samariscus and Samaris. Studies on mitochondrial genomes of Samaris cristatus and Samariscus latus showed that there exist differences in genomic rearrangement and gene number between this two species. To determine whether there are structural differences in the species of the genus Plagiopsetta, Plagiopsetta glossa were used as the representative species for this study, and the mitochondrial genomic characteristics of this species were compared with those of Samariscus and Samaris. The length of its mitogenome is 18,723 bp, and contains 39 genes, including 13 protein-coding genes, two rRNA genes, 24 tRNA genes, two control regions, one light strand replication origin, and 13 more interspaces. Compared with the typical mitogenome in teleosts, P. glossa and S. latus have two more genes of tRNA-Cys and tRNA-Tyr (S. cristatus only one more tRNA-Cys). Additionally, each of the three flatfish has an extra control region, but the gene orders of P. glossa and S. latus mitogenomes are the same. Six genes (tRNA-Cys1, tRNA-Tyr1, tRNA-Ser1, tRNA-Lys, tRNA-Arg, and tRNA-Ser2) from different locations are clustered together forming a gene cluster, and following by this genes cluster was ND5-ND6-Glu-Cytb-Thr, all those 11 genes have no gene order change in terms of typical mitogenome.

The model of Double Replications and Random Loss was used to analyze the possible rearrangement mechanism in P. glossa mitogenome. The characteristics of gene number, gene order and 13 more interspaces provided new evidence to support the applicability of this model. The results of this study not only enrich our scientific knowledge of mitogenomic features, but also provide more data for further study on mitochondrial evolution and phylogenetic analysis for flatfish.

Key words: Pleuronectiformes, Samaridae, mitogenome, gene rearrangement, molecular evolution

中图分类号: 

  • P735.541