对凡纳滨对虾Litopenaeus vannamei精子发生的过程进行了透射电镜的观察。结果表明, 成熟凡纳滨对虾精巢为指状, 分16叶。凡纳滨对虾精子发生分为精原细胞、初级精母细胞、次级精母细胞、精子细胞和精子5个时期。染色质经历了从以异染色质为主变为高度凝聚状态, 再经解聚为弥散絮状的变化过程。核膜由完整转变为不完整。凡纳滨对虾精子发生过程中仅见线粒体、内质网、核糖体细胞器的参与, 未见有典型的高尔基体结构的出现。顶体由内质网囊泡团和线粒体囊泡共同转变而来。精子的棘突以及亚顶体区需在输精管的中后端形成, 而非在精巢中形成。纳精囊内精子比储精囊内精子的棘突细长。

Spermatogenesis in shrimp Litopenaeus vannamei was investigated using light and transmission electron microscopy. Results showed that the matured testis of L. vannamei was fingers-like, which was comprised of 16 leaves. Spermatogenesis in L. vannamei could be divided into five stages: spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid and sperm. During spermatogenesis, chromatins underwent dramatic changes. At the stage of spermatogonium, the heterochromatins were spread in the nucleus and some of them were distributed under the nuclear membrane. At the stage of spermatocyte, chromatins became highly coacervative. At the stage of spermatid and sperm, the chromatins lost condensation and looked like flocculus. Mitochondria, endoplasmic reticulum and ribosome were observed during spermatogenesis. No typical golgi apparatus were observed. The acrosome was derived from endoplasmic reticulum vesicles and mitochondria vesicles. The sperm spike and sub-acrosome were formed in the vas deferens, not in the testis. The sperm spike from the seminal receptacle was longer than that from the terminal ampullae.

国家“863”计划子课题(2006AA10A406、2010AA10A401); 广东省海洋渔业科技推广专项(A200899A02、A200901B03); 中国科学院广东省科学院院合作项目(2009B091300088)

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