Journal of Tropical Oceanography >
Diversity and anti-bacteria activity of the gorgonian derived fungi from Weizhou Island of Guangxi Province*
Copy editor: YIN Bo
Received date: 2020-10-30
Request revised date: 2021-01-27
Online published: 2021-01-25
Supported by
National Natural Science Foundation of China(42066006)
National Natural Science Foundation of China(41606186)
Guangxi Natural Science Foundation(AD19245116)
Development Program of High level Talent Team under Qihuang Project of Guangxi University of Chinese Medicine(2018006)
Special Fund for Bagui Scholars of Guangxi(05019055)
Initial Scientific Research Foundation of Introduced Doctors in 2019 of GXUCM(2018BS039)
Scientific Research Foundation of Institute of Marine Drugs, GUCM(2018ZD005)
Guangxi First-class Discipline: Chinese Materia Medica(Scientific Research of Guangxi Education Department)([2018] No. 12)
Copyright
Four media were used to study culturable fungal diversity in 10 gorgonian samples obtained from Weizhou Island, Guangxi Province. Fungi were further identified by colonial morphology observations and molecular biological analyses. Their anti-bacteria and anti-biofilm activities were determined by 96-well microplate assay. A total of 191 fungal strains were isolated from those gorgonians. All of the 191 fungi belonged to the phylum Ascomycota, and were distributed among six genera: Aspergillus, Penicillium, Alternaria, Cladosporium, Nigrospora, and Parengyodontium. Eleven fungal strains exhibited inhibitory effect against the three strains of pathogenic bacteria, which are Staphylococcus epidermidis ATCC12228, Streptococcus agalactiae ATCC51487, and Streptococcus iniae ATCC29178. The strains P. cinnamopurpureum GXIMD00518 and A. carneus GXIMD00519 also showed anti-MRSA biofilm activities. Gorgonians are rich resources of fungi that deserve further exploration.
Key words: gorgonian; marine derived fungus; biodiversity; anti-bacteria activity; biofilm
LU Chunju , LU Meilin , LIU Xinming , LIU Yonghong , GAO Chenghai , XU Xinya . Diversity and anti-bacteria activity of the gorgonian derived fungi from Weizhou Island of Guangxi Province*[J]. Journal of Tropical Oceanography, 2021 , 40(5) : 45 -52 . DOI: 10.11978/2020124
表1 10株柳珊瑚共附生真菌的分离数量Tab. 1 Numbers of isolates from the 10 gorgonians |
柳珊瑚样本编号 | 共附生真菌分离数 | 总计 | |||
---|---|---|---|---|---|
虎红培养基 | 查氏培养基 | 马铃薯葡萄糖琼脂培养基 | 沙氏培养基 | ||
1 | 10 | 7 | 5 | 14 | 36 |
2 | 7 | 10 | 6 | 6 | 29 |
3 | 3 | 4 | 3 | 0 | 10 |
4 | 12 | 5 | 7 | 9 | 33 |
5 | 0 | 0 | 1 | 0 | 1 |
6 | 1 | 1 | 4 | 1 | 7 |
7 | 3 | 1 | 3 | 0 | 7 |
8 | 12 | 10 | 7 | 1 | 30 |
9 | 8 | 8 | 5 | 7 | 28 |
10 | 4 | 3 | 3 | 0 | 10 |
总计 | 60 | 49 | 44 | 38 | 191 |
表2 基于ITS序列分析鉴定的柳珊瑚共附生真菌Tab. 1 Identification of fungal strains isolated from gorgonians based on DNA analysis of the ITS region |
菌株编号 | GenBan登录号 | 序列长度/bp | 最相近菌株(BLAST) | 相似度/% | GenBan登录号 |
---|---|---|---|---|---|
GXIMD00501 | MT672589 | 533 | Parengyodontium album | 100 | MN944461.1 |
GXIMD00502 | MT672603 | 535 | Aspergillus niger | 100 | KX622774.1 |
GXIMD00503 | MT672604 | 516 | Nigrospora oryzae | 100 | KT824761.1 |
GXIMD00504 | MT672605 | 508 | Cladosporium sp. | 99.80 | MH325933 |
GXIMD00505 | MT707929 | 515 | Cladosporium sp. | 99.61 | KT989416.1 |
GXIMD00506 | MT672606 | 511 | Cladosporium halotolerans | 100 | KU216734.1 |
GXIMD00507 | MT672611 | 512 | Penicillium citrinum | 100 | MK736919.1 |
GXIMD00508 | MT672612 | 515 | Alternaria alternata | 100 | MK518403.1 |
GXIMD00509 | MT672613 | 541 | Aspergillus micronesiensis | 99.82 | MT312759.1 |
GXIMD00510 | MT672614 | 557 | Aspergillus flavipes | 99.64 | MN956655.1 |
GXIMD00511 | MT672615 | 530 | Aspergillus wentii | 100 | MH855236.1 |
GXIMD00512 | MT672616 | 540 | Aspergillus sp. | 99.81 | EF672305.1 |
GXIMD00513 | MT672617 | 518 | Aspergillus sp. | 100 | MH665645.1 |
GXIMD00514 | MT672618 | 518 | Aspergillus sp. | 100 | MK431431.1 |
GXIMD00515 | MT672619 | 575 | Aspergillus penicillioides | 99.83 | GU017494.1 |
GXIMD00516 | MT672620 | 502 | Aspergillus sydowii | 100 | MH793853.1 |
GXIMD00518 | MT672622 | 509 | Penicillium cinnamopurpureum | 100 | KU847973.1 |
GXIMD00519 | MT672623 | 513 | Aspergillus carneus | 99.81 | MH777426.1 |
GXIMD00520 | MT672624 | 508 | Aspergillus tabacinus | 100 | MT635280.1 |
GXIMD00521 | MT672625 | 519 | Aspergillus unguis | 100 | MH654997.1 |
GXIMD00522 | MT672626 | 519 | Aspergillus restrictus | 100 | KT959332.1 |
GXIMD00517 | MT672621 | 540 | Aspergillus versicolor | 99.81 | LC105686.1 |
GXIMD00523 | MT742624 | 519 | Aspergillus versicolor | 100 | KX674616.1 |
GXIMD00524 | MT742625 | 514 | Aspergillus versicolor | 99.81 | MF476045.1 |
GXIMD00525 | MT742626 | 532 | Aspergillus versicolor | 100 | KX776009.1 |
GXIMD00526 | MT742624 | 517 | Aspergillus versicolor | 99.81 | MN310533.1 |
表3 真菌发酵液乙酸乙酯提取物抑制细菌活性实验结果Tab. 3 Antibacterial activities of ethyl acetate (EtOAc) extracts from fermentation broth of the gorgonian-derived fungal strains |
菌株 | 抑制率/% | ||
---|---|---|---|
表皮葡萄球菌 | 无乳链球菌 | 海豚链球菌 | |
Penicillium citrinum GXIMD00507 | 77.44 | 78.35 | 86.36 |
Alternaria alternata GXIMD00508 | 63.64 | - | - |
Aspergillus micronesiensis GXIMD00509 | 70.99 | 58.77 | 68.24 |
Aspergillus flavipes GXIMD005010 | 57.60 | - | - |
Aspergillus wentii GXIMD00511 | 76.90 | 69.33 | 81.22 |
Aspergillus sydowii GXIMD00516 | 62.96 | - | 67.22 |
Aspergillus versicolor GXIMD00517 | 58.42 | 62.05 | 80.02 |
Penicillium cinnamopurpureum GXIMD00518 | 86.48 | 79.81 | 85.52 |
Aspergillus carneus GXIMD00519 | 59.25 | - | 76.38 |
Aspergillus tabacinus GXIMD00520 | 58.56 | - | 65.50 |
Aspergillus unguis GXIMD00521 | 88.17 | 80.57 | 86.19 |
青霉素 | 91.44 | 73.93 | 84.93 |
注: 青霉素为阳性对照, 浓度为40μg•mL-1; “-”表示菌株在400μg•mL-1浓度时抑菌率小于50% |
表4 真菌发酵液乙酸乙酯提取物抗生物被膜活性实验结果Tab. 4 Anti-biofilm activities of EtOAc extracts from fermentation broth of the gorgonian-derived fungal strains |
菌株 | 耐甲氧西林金黄色葡萄球菌 | 表皮葡萄球菌 | |||
---|---|---|---|---|---|
MIC50/(μg•mL-1) | EC50/(μg•mL-1) | MIC50/(μg•mL-1) | EC50/(μg•mL-1) | ||
Penicillium citrinum GXIMD00507 | >200 | - | >200 | 150.6 | |
Aspergillus micronesiensis GXIMD00509 | >200 | - | >200 | 154.8 | |
Aspergillus wentii GXIMD00511 | >200 | - | >200 | 40.3 | |
Penicillium cinnamopurpureum GXIMD00518 | >200 | 105.4 | >200 | 102.9 | |
Aspergillus carneus GXIMD00519 | >200 | 117.4 | >200 | 105.0 | |
Aspergillus tabacinus GXIMD00520 | >200 | - | >200 | 66.3 | |
Aspergillus versicolor GXIMD00525 | >200 | - | >200 | 155.0 | |
青霉素 | 6.67 | - | - | - |
注: 青霉素为阳性对照, 浓度范围为2.5~40μg•mL-1; “-”表示提取物在200μg•mL-1浓度时, 对生物被膜的抑制率小于50% |
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