Journal of Tropical Oceanography >
Studies on the ultrastructure of spermatogenesis in shrimp, Litopenaeus vannamei
Received date: 2009-07-25
Revised date: 2009-09-18
Online published: 2010-07-29
Supported by
国家“863”计划子课题(2006AA10A406、2010AA10A401); 广东省海洋渔业科技推广专项(A200899A02、A200901B03); 中国科学院广东省科学院院合作项目(2009B091300088)
Spermatogenesis in shrimp Litopenaeus vannamei was investigated using light and transmission electron microscopy. Results showed that the matured testis of L. vannamei was fingers-like, which was comprised of 16 leaves. Spermatogenesis in L. vannamei could be divided into five stages: spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid and sperm. During spermatogenesis, chromatins underwent dramatic changes. At the stage of spermatogonium, the heterochromatins were spread in the nucleus and some of them were distributed under the nuclear membrane. At the stage of spermatocyte, chromatins became highly coacervative. At the stage of spermatid and sperm, the chromatins lost condensation and looked like flocculus. Mitochondria, endoplasmic reticulum and ribosome were observed during spermatogenesis. No typical golgi apparatus were observed. The acrosome was derived from endoplasmic reticulum vesicles and mitochondria vesicles. The sperm spike and sub-acrosome were formed in the vas deferens, not in the testis. The sperm spike from the seminal receptacle was longer than that from the terminal ampullae.
Key words: Litopenaeus vannamei; spermatogenesis; acrosome; ultrastructure
XU You-hou,LIU Xue-dong,ZHANG Lü-ping,HU Chao-qun . Studies on the ultrastructure of spermatogenesis in shrimp, Litopenaeus vannamei[J]. Journal of Tropical Oceanography, 2010 , 29(4) : 89 -93 . DOI: 10.11978/j.issn.1009-5470.2010.04.089
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