热带海洋学报

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牡蛎酶解产物对产后缺乳症泌乳功能改善作用探究

韦雪1,秦小明1,2*,谌素华1,张锴佳1,林海生1,2,郑惠娜1,2,髙加龙1,2   

  1. 1. 广东海洋大学食品科技学院,国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省亚热带果蔬加工科技创新中心,广东湛江,524088;

    2. 海洋食品精深加工关键技术省部共建协同创新中心(大连工业大学),辽宁 大连,116034


  • 收稿日期:2022-04-11 修回日期:2022-06-17 出版日期:2022-06-30 发布日期:2022-06-30
  • 通讯作者: 秦小明
  • 基金资助:
    财政部和农业农村部国家现代农业产业技术体系

Study on the effect of Oyster hydrolysates on improving Lactation function in Postpartum hypogalactism

WEI Xue1, QIN Xiaoming1,2*, CHEN Suhua1, ZHANG Kaijia1, LIN Haisheng1,2, ZHENG Huina1,2, GAO Jialong1,2   

  1. 1. Processing (Zhanjiang), Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, China;

    2. Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University,Dalian 116034

  • Received:2022-04-11 Revised:2022-06-17 Online:2022-06-30 Published:2022-06-30
  • Contact: QIN XIAOMING
  • Supported by:

    he China Agricultural Research System of MOFand MARA

摘要: 摘 要: 本文以香港牡蛎(Crassostrea hongkongensis)为原料制备牡蛎酶解产物(Oyster Hydrolysis. OH),分析了其基本营养成分和氨基酸组成,并探究其对甲磺酸溴隐亭致产后缺乳症大鼠的泌乳功能改善作用。结果表明,OH粗蛋白含量较高,其次是总糖,而脂肪含量较低,肽含量为33.4%;OH中必需氨基酸占总氨基酸的39.8%,且含有较高比例的支链氨基酸。动物实验结果表明,相较于模型组,OH高剂量组大鼠泌乳量显著增加(P<0.05)且优于阳性对照组;OH各剂量组均极显著提高了缺乳大鼠乳腺组织器官指数(P<0.01),其效果接近于阳性对照组,但未能恢复到正常水平;HE染色观察结果显示,OH各剂量组乳腺组织腺泡较多,腺泡呈现圆形且充盈,内有较多的分泌物,腺泡有明显扩大增生,相较于模型组,有明显的恢复;与模型组比较,OH各剂量组血清和乳腺组织催乳素(PRL)、催乳素受体(PRLR)含量显著提高(P<0.05),说明OH能够促进缺乳大鼠催乳素分泌,而血清多巴胺(DA) 的含量极显著降低(P<0.01),表明OH能够降低由溴隐亭导致的多巴胺含量过高。 此外,OH能显著提高乳腺组织中乳糖(LC)、甘油三酯(TG)含量(P<0.05),提高了乳汁营养成分;OH各剂量组血液中平均血红蛋白(MCHC)、红细胞数(RBC)的含量也显著增加(P<0.05),对产后气血不足有一定的改善作用。结论:OH能够改善缺乳症状,提高产后泌乳量,具有促进泌乳的潜力。

关键词: 牡蛎酶解产物, 产后缺乳, 甲磺酸溴隐亭, 泌乳量

Abstract: Abstract: In this paper, Oyster Hydrolysis products(OH) were prepared from Crassostrea hongkongensis as raw materials, and the basic nutrition and amino acid composition of OH were analyzed, and the improvement effect of OH on the lactation function of rats with postpartum lactation caused by Bromocriptine mesylate was explored.The results showed that OH protein content was higher, followed by sugar, fat content was lower, and peptide content was 33.4%.??The essential amino acids in OH accounted for 39.8% of the total amino acids and contained a high proportion of branched amino acids.??The results of animal experiment showed that compared with model group, the lactation volume of rats in OH high-dose group was significantly increased (P < 0.05), which was better than that in positive control group. The index of mammary tissue and organ in each OH dose group was significantly increased (P<0.01), which was close to the positive control group, but did not return to normal level. HE staining showed that there were more acinus in mammary gland tissues in all OH dose groups, which were round and full with more secretions, and the acinus had obvious enlargement and hyperplasia. Compared with the model group, the acinus had obvious recovery. Compared with model group, the contents of prolactin (PRL) and prolactin receptor (PRLR) in serum and mammary tissue of OH groups were significantly increased (P< 0.05), indicating that OH could promote the secretion of prolactin in lactation-deficient rats, while the content of serum dopamine (DA) was significantly decreased (P<0.01). These results showed that OH could reduce the high level of dopamine induced by bromocystine. In addition, OH significantly increased the contents of lactose (LC) and triglyceride (TG) in breast tissue (P<0.05), and improved the nutritional composition of milk. The average hemoglobin (MCHC) and red blood cell number (RBC) contents in all OH dose groups were also significantly increased (P<0.05), which had a certain improvement effect on postpartum deficiency of qi and blood. Conclusion: OH can improve the symptoms of lactation deficiency and increase milk yield after delivery, which has the potential to promote lactation.

Key words: Oyster hydrolysates, postpartum lactation deficiency, bromocriptine mesylate, amount of lactation