热带海洋学报 ›› 2010, Vol. 29 ›› Issue (3): 55-60.doi: 10.11978/j.issn.1009-5470.2010.03.055

• 海洋生物学 • 上一篇    下一篇

34株副溶血弧菌16S-23S rDNA间区多态性DGGE分析

苏婷, 罗鹏, 胡超群, 任春华   

  1. 中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室, 广东 广州 510301
  • 收稿日期:2008-03-25 修回日期:2008-12-02 出版日期:2010-07-01 发布日期:2010-05-24
  • 作者简介:苏婷(1985—), 女, 广西桂林市人, 硕士研究生, 从事海洋微生物学研究.
  • 基金资助:

    国家“973”计划项目(2006CB101803); 国家自然科学基金(30700016)

Analysis of the polymorphism of 16S-23S rDNA intergenic spacer regions from 34 Vibrio parahaemolyticus strains by denaturing gradient gel electrophoresis

SU Ting, LUO Peng, HU Chao-qun, REN Chun-hua   

  1. South China Sea Institute of Oceanology, Key Laboratory of Marine Bio-resources Sustainable Utilization, cas, Key Laboratory of Applied Marine Biology of Guangdong, Guangzhou 510301
  • Received:2008-03-25 Revised:2008-12-02 Online:2010-07-01 Published:2010-05-24
  • About author:苏婷(1985—), 女, 广西桂林市人, 硕士研究生, 从事海洋微生物学研究.
  • Supported by:

    国家“973”计划项目(2006CB101803); 国家自然科学基金(30700016)

摘要:

采用PCR-变性梯度凝胶电泳技术(PCR-DGGE)分析比较了34株分离于环境和水产养殖动物体内的副溶血弧菌及标准株16S-23S rDNA间区(Intergenic Spacer Region, ISR)的多态性和亲缘关系。结果表明, 34株副溶血弧菌的ISR经PCR-DGGE电泳后均能分离出4―10条条带, 共计产生15个多态性位点。所有菌株聚为H、I、J、K四大簇, 株间遗传差异最大为株A18和A25, 遗传距离达到0.4。ISR PCR-DGGE方法为副溶血弧菌基因分型提  供了一种新的方法。

关键词: 副溶血弧菌, 16S-23S rDNA间区, PCR-DGGE

Abstract:

Using PCR-denaturing gradient gel electrophoresis (PCR-DGGE), the polymorphism of 16S-23S rDNA intergenic spacer regions (ISR) from 34 Vibrio parahaemolyticus strains, including type strains and those isolated from environment and aquatic animals, were analyzed. The phylogenic relationships of these strains were also analyzed. The results showed that the ISRs could be separated into 4−10 different bands through PCR-DGGE. Thirty-four V. parahaemolyticus strains shared 15 polymorphic sites. Using MVSP, 34 strains were clustered into four groups, H, I, J, and K. Strains A18 and A25 had farthest genetic distance, with a value of 0.4. The present study suggested that ISR PCR-DGGE provided a new method for genotyping of V. parahaemolyticus.

Key words: Vibrio parahaemolyticus, 16S-23S rDNA intergenic spacer Regions, PCR-DGGE