牡蛎酶解产物对产后缺乳症泌乳功能改善作用探究

doi:10.11978/2022075

热带海洋学报 ›› 2023, Vol. 42 ›› Issue (2): 141-152.doi: 10.11978/2022075CSTR: 32234.14.2022075

牡蛎酶解产物对产后缺乳症泌乳功能改善作用探究

韦雪¹(), 秦小明¹^,²(), 谌素华¹, 张锴佳¹, 林海生¹^,², 郑惠娜¹^,², 高加龙¹^,²

1.广东海洋大学食品科技学院, 国家贝类加工技术研发分中心(湛江), 广东省水产品加工与安全重点实验室, 广东省海洋生物制品工程实验室, 广东省亚热带果蔬加工科技创新中心, 广东湛江 524088
2.海洋食品精深加工关键技术省部共建协同创新中心(大连工业大学), 辽宁大连 116034

收稿日期:2022-04-11 修回日期:2022-06-17 出版日期:2023-03-10 发布日期:2022-06-30
通讯作者: 秦小明。email: xiaoming0502@21cn.com
作者简介:
韦雪(1996—), 女, 广西壮族自治区钦州市人, 硕士研究生, 从事食品加工与安全。email: weixue03212022@163.com
基金资助:
财政部和农业农村部国家现代农业产业技术项目(CARS-49)

Study on the effect of Oyster hydrolysates on improving Lactation function in Postpartum hypogalactism

WEI Xue¹(), QIN Xiaoming¹^,²(), CHEN Suhua¹, ZHANG Kaijia¹, LIN Haisheng¹^,², ZHENG Huina¹^,², GAO Jialong¹^,²

1. College of Food Science and Technology, Guangdong Ocean University, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, China
2. Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China

Received:2022-04-11 Revised:2022-06-17 Online:2023-03-10 Published:2022-06-30
Contact: QIN Xiaoming. email: xiaoming0502@21cn.com
Supported by:
China Agricultural Research System of MOF and MARA(CARS-49)

摘要/Abstract

摘要：

文章以香港牡蛎(Crassostrea hongkongensis)为原料制备牡蛎酶解产物(oyster hydrolysis, OH), 分析其基本营养成分和氨基酸组成, 并探究其对甲磺酸溴隐亭致产后缺乳症大鼠的泌乳功能改善作用。结果表明: OH粗蛋白含量较高, 其次是总糖, 而脂肪含量较低, 肽含量为33.4%; OH中必需氨基酸占总氨基酸的39.8%, 且含有较高比例的支链氨基酸。动物试验结果表明: 相较于模型组, OH高剂量组大鼠泌乳量显著增加(p < 0.05)且优于阳性对照组; OH各剂量组均极显著提高了缺乳大鼠乳腺组织器官指数(p < 0.01), 其效果接近于阳性对照组, 但未能恢复到正常水平; 苏木精-伊红染色观察结果显示, OH各剂量组乳腺组织腺泡较多, 腺泡呈现圆形且充盈, 内有较多的分泌物, 腺泡有明显扩大增生, 相较于模型组, 有明显的恢复; 与模型组比较, OH各剂量组血清和乳腺组织催乳素(prolactin, PRL)、催乳素受体(prolactin receptor, PRLR)含量显著提高(p < 0.05), 说明OH能够促进缺乳大鼠催乳素分泌, 而血清多巴胺(dopamine, DA) 的含量极显著降低(p < 0.01), 表明OH能够降低由溴隐亭导致的多巴胺含量过高。此外, OH能显著提高乳腺组织中乳糖(lactose, LC)、甘油三酯(triglyceride, TG)含量(p < 0.05), 提高了乳汁营养成分; OH各剂量组血液中平均血红蛋白(mean corpuscular hemoglobin concentration, MCHC)、红细胞(red blood cell, RBC)的数量也显著增加(p < 0.05), 对产后气血不足有一定的改善作用。因此, OH能够改善缺乳症状, 提高产后泌乳量, 具有促进泌乳的潜力。

关键词: 牡蛎酶解产物, 产后缺乳, 甲磺酸溴隐亭, 泌乳量

Abstract:

In this paper, oyster hydrolysis products were prepared from Crassostrea hongkongensis as raw materials, and the basic nutrition and amino acid composition of oyster hydrolysis products were analyzed, and the improvement effect of oyster hydrolysis products on the lactation function of rats with postpartum lactation caused by Bromocriptine mesylate was explored. The results showed that protein content of oyster hydrolysis products was higher, which is followed by sugar, fat content was lower, and peptide content was 33.4%. The essential amino acids in oyster hydrolysis products accounted for 39.8% of the total amino acids and contained a high proportion of branched amino acids. The results of animal experiment showed that compared with model group, the lactation volume of rats in oyster hydrolysis products of high-dose group was significantly increased (p < 0.05), which was better than that in the positive control group. The index of mammary tissue and organ in each oyster hydrolysis products dose group was significantly increased (p < 0.01), which was close to the positive control group, but did not return to normal level. HE staining showed that there were more acinus in mammary gland tissues in all oyster hydrolysis products dose groups, which were round and full with more secretions, and the acinus had obvious enlargement and hyperplasia. Compared with the model group, the acinus had obvious recovery. Compared with model group, the contents of prolactin (PRL) and prolactin receptor (PRLR) in serum and mammary tissue of oyster hydrolysis products groups were largely increased (p < 0.05), indicating that oyster hydrolysis products could promote the secretion of prolactin in lactation-deficient rats, while the content of serum dopamine (DA) was greatly decreased (p < 0.01). These results showed that oyster hydrolysis products can reduce the high level of dopamine induced by bromocystine. In addition, oyster hydrolysis products strongly increased the contents of lactose (LC) and triglyceride (TG) in breast tissue (p < 0.05), and improved the nutritional composition of milk. The average hemoglobin (MCHC) and red blood cell (RBC) contents in all oyster hydrolysis products dose groups were also greatly increased (p < 0.05), which had a certain improvement effect on postpartum deficiency of qi and blood. Conclusion: Oyster hydrolysis products can improve the symptoms of lactation deficiency and increase milk yield after delivery, which has the potential to promote lactation.

Key words: oyster hydrolysates, postpartum lactation deficiency, bromocriptine mesylate, amount of lactation

韦雪, 秦小明, 谌素华, 张锴佳, 林海生, 郑惠娜, 高加龙. 牡蛎酶解产物对产后缺乳症泌乳功能改善作用探究[J]. 热带海洋学报, 2023, 42(2): 141-152.

WEI Xue, QIN Xiaoming, CHEN Suhua, ZHANG Kaijia, LIN Haisheng, ZHENG Huina, GAO Jialong. Study on the effect of Oyster hydrolysates on improving Lactation function in Postpartum hypogalactism[J]. Journal of Tropical Oceanography, 2023, 42(2): 141-152.

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组别	母鼠数量/只	每窝仔鼠数量/只	处理方式
空白组(B组)	6	12	用1mL·100g^-1蒸馏水灌胃, 每天一次
模型组(M组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1mL·100g^-1)和1mL·100g^-1蒸馏水灌胃, 每天一次
阳性药物组(P组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1mL·100g^-1)和补血生乳颗粒(浓度267mg·mL^-1、体积1mL·100g^-1)灌胃, 每天一次
牡蛎酶解产物低剂量组(OH-L组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1mL·100g^-1)和牡蛎酶解粉(浓度31.25mg·mL^-1、体积1mL·100g^-1)灌胃, 每天一次
牡蛎酶解产物中剂量组(OH-M 组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1L·100g^-1)和牡蛎酶解粉(浓度62.5mg·mL^-1、体积1 mL·100g^-1)灌胃, 每天一次
牡蛎酶解产物高剂量组(OH-H 组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1mL·100g^-1)和牡蛎酶解粉(浓度125mg·mL^-1、体积1mL·100g^-1)灌胃, 每天一次

种类	水解氨基酸含量/(g·100g^-1)	游离氨基酸含量/(g·100g^-1)
天门冬氨酸(ASP)	3.81	0.74
苏氨酸(THR)*	1.67	1.37
丝氨酸(SER)	1.80	1.00
谷氨酸(GLU)	5.94	1.97
脯氨酸(PRO)	1.46	0.59
甘氨酸(GLY)	2.45	0.65
丙氨酸(ALA)	2.20	1.56
缬氨酸(VAL)*	2.16	1.30
蛋氨酸(MET)*	1.34	0.97
异亮氨酸(ILE)*	1.79	1.43
亮氨酸(LEU)*	2.79	2.39
酪氨酸 TYR	1.04	2.09
苯丙氨酸(PHE)*	1.57	2.02
赖氨酸(LYS)*	2.84	0.92
组氨酸(HIS)	0.48	0.27
精氨酸(ARG)	2.31	1.52
氨基酸总和(TAA)	35.60	20.80
必需氨基酸(EAA)	14.16	10.40
非必需氨基酸(NEAA)	21.44	10.40
EAA与TAA比值/%	39.80	50.00
EAA与NEAA比值/%	66.00	100.00

指标	B组	M组	P组	OH-L组	OH-M组	OH-H组
心脏指数	0.45±0.11	0.43±0.03	0.43±0.05	0.40±0.03	0.44±0.05	0.43±0.06
胸腺指数	0.09±0.02	0.11±0.02	0.12±0.01	0.11±0.01	0.11±0.02	0.12±0.02
脾脏指数	0.22±0.01	0.24±0.02	0.24±0.02	0.23±0.01	0.25±0.02	0.25±0.04
肝脏指数	3.94±0.42	3.41±0.37	3.20±0.22	3.20±0.24	3.28±0.21	3.41±0.21
肾脏指数	0.70±0.07	0.74±0.07	0.74±0.04	0.75±0.08	0.72±0.06	0.71±0.05

时间	B组	M组	P组	OH-L组	OH-M组	OH-H组
第1天	2.28±0.73	1.49±0.40^##	3.35±0.50^**	1.75±1.94	2.51±0.48^**	2.93±0.50^**
第2天	2.29±1.04	2.57±1.04	2.07±0.86	0.81±1.82	1.21±0.41	3.19±0.32
第3天	4.11±0.79	1.98±1.26^#	1.60±0.77	2.33±0.77	0.89±0.83	2.02±0.57
第4天	4.46±0.56	0.90±1.13^##	2.17±0.77	0.71±1.42	1.99±0.88	1.74±0.12
第5天	6.38±1.15	0.44±0.48^##	1.05±0.98	1.77±1.24	0.67±0.41	2.47±1.00^**
第6天	6.20±1.1	0.03±0.48^##	1.82±0.31^**	1.05±1.39	0.13±0.66	2.79±0.89^**
第7天	4.88±1.62	0.39±0.51^##	0.05±0.79	0.57±1.29	1.00±0.79	3.10±0.85^**
第8天	4.74±1.45	0.05±0.10^##	1.64±2.10^**	0.93±1.48	0.18±0.23	1.11±0.70

组别	第3天	第4天	第5天	第6天	第7天	第8天
B组	100±0.00	100±0.00	100±0.00	100±0.00	100±0.00	100±0.00
M组	100±0.00	100±0.00	94.44±8.61	88.88±6.21^##	84.72±3.40^##	77.78±4.30^##
P组	100±0.00	100±0.00	100±0.00	98.61±3.40^*	95.84±4.56^**	87.50±4.57^**
OH-L组	100±0.00	97.22±4.30	98.61±3.40	98.61±3.40^*	87.50±4.57^##△△	81.94±3.40^△
OH-M组	100±0.00	100±0.00	98.61±3.40	95.84±4.56	94.45±4.30^**	84.72±3.40^**
OH-H组	100±0.00	100±0.00	100±0.00	100±0.00^**	98.61±3.40^**	93.06±3.40^**△

牡蛎酶解产物对产后缺乳症泌乳功能改善作用探究

Study on the effect of Oyster hydrolysates on improving Lactation function in Postpartum hypogalactism

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