三种红树根部组织抗氧化酶活性对水淹胁迫的响应

doi:10.11978/2021107

热带海洋学报 ›› 2022, Vol. 41 ›› Issue (6): 35-43.doi: 10.11978/2021107CSTR: 32234.14.2021107

三种红树根部组织抗氧化酶活性对水淹胁迫的响应

苏柏予¹^,²^,³^,⁴^,⁵(), 张维仕⁶, 王友绍¹^,²^,³^,⁴()

1.热带海洋环境国家重点实验室(中国科学院南海海洋研究所), 广东广州 510301
2.中国科学院大亚湾海洋生物综合试验站, 广东深圳 518121
3.南方海洋科学与工程广东省实验室(广州), 广东广州 511458
4.中国科学院南海生态环境工程创新研究院, 广东广州 510301
5.中国科学院大学, 北京 100049
6.临沂市白沙埠中学, 山东临沂 276035

收稿日期:2021-08-21 修回日期:2021-10-12 出版日期:2022-11-10 发布日期:2021-10-14
通讯作者: 王友绍
作者简介:苏柏予(1997—), 女, 广西梧州市人, 硕士研究生, 从事红树林生态学研究。email: subaiyu82@163.com
基金资助:
国家重点研发计划项目(国家科技基础资源调查专项)(2017FY100700);国家自然科学基金重点项目(U1901211);国家自然科学基金重点项目(41876126);中国科学院A类战略性先导科技专项(XDA23050200);国际伙伴计划(133244KYSB20180012);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0305)

Response of antioxidant enzyme systems in root tissues of three mangrove species to waterlogging stress

SU Boyu¹^,²^,³^,⁴^,⁵(), ZHANG Weishi⁶, WANG Youshao¹^,²^,³^,⁴()

1. State Key Laboratory of Tropical Oceanography (South China Sea Institute of Oceanology, Chinese Academy of Sciences), Guangzhou 510301, China
2. Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518121, China
3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China
4. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou 510301, China
5. University of Chinese Academy of Sciences, Beijing 100049, China
6. Linyi Baishabu Middle school, Linyi 276035, China

Received:2021-08-21 Revised:2021-10-12 Online:2022-11-10 Published:2021-10-14
Contact: WANG Youshao
Supported by:
National Key Research and Development Program of China(2017FY100700);National Natural Science Foundation of China(U1901211);National Natural Science Foundation of China(41876126);Strategic Priority Research Program of the Chinese Academy of Sciences(XDA23050200);International Partnership Program of Chinese Academy of Sciences(133244KYSB20180012);Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0305)

摘要/Abstract

摘要：

以自然种群高程分布为根据, 选择对潮汐浸泡耐性存在一定差异的白骨壤(Avicennia marina)、桐花树(Aegiceras corniculatum)和木榄(Bruguiera gymnorrhiza)为实验树种。对3种红树幼苗进行为期0d、1d、3d、5d、7d、14d的水淹处理, 测定其根部组织的过氧化物酶(peroxidase, POD)活性、超氧化物歧化酶(superoxide dismutase, SOD)活性、抗坏血酸氧化酶(ascorbic acid oxidase, APX)活性和过氧化氢酶(catalase, CAT)活性。水淹处理会导致植物根部组织受低氧胁迫从而破坏活性氧(reactive oxygen species, ROS)生产和消除的平衡, ROS大量累积给植物带来氧化损伤。实验测定与清除ROS有关的酶指标, 结果表明POD活性在1d至14d的水淹周期内随时间呈现上升趋势, 这可能与POD参与活性氧以及乙醇、乳酸等有毒物质的反应有关。CAT、SOD、APX活性均呈现应激上升后下降趋势, 由此推断该系列抗氧化酶在水淹胁迫发生前中期对消除红树植物根部组织大量产生的活性氧具有重要意义。抗氧化酶系在3种红树植物中的表达存在一定差异, 但整体仍呈相似的变化趋势。通过层次聚类法对各项酶活性指标分析后发现, 白骨壤抗氧化酶系的活性模式与木榄和桐花树的存在一定差异, 但没有发现木榄与桐花树抗氧化酶系统表达模式的明显差异。本研究旨在更好地了解红树林对水淹胁迫的生理适应机制, 为红树林生态系统保护与管理提供理论依据。

关键词: 红树植物, 水淹胁迫, 抗氧化酶系统, 聚类分析

Abstract:

Based on the natural distribution of different mangrove populations, Avicennia marina, Aegiceras corniculatum and Bruguiera gymnorrhiza were chosen to be the experimental species that have different resistance of water-logging. The activities of peroxidase (POD), superoxide dismutase (SOD), ascorbate oxidase (APX) and catalase (CAT) in root tissues of three species of mangrove were tested after submerging for 0d, 1d, 3d, 5d, 7d and 14d. Under hypoxia environment, the balances of production and elimination of reactive oxygen species (ROS) would be destroyed and a large amount of ROS were accumulated. The results showed that POD increased with time during the period from 1d to 14d, which may be related to the involvement of scavenging of toxic substances in addition to the elimination of reactive oxygen. The CAT, SOD and APX increased when mangroves encounter the water-logging and then decreased. The enzymes play an important role in eliminating the large amount of ROS in the root tissues of mangrove plants during the early period of the waterlogging stress. There were some differences in the expression quantity of antioxidant enzyme system among the three mangrove plants but the fluctuations are similar. Hierarchical clustering analysis of various enzyme activity indexes indicated that the activity pattern of antioxidant enzyme system in A.marina was different from that in A. corniculatum and B. gymnorrhiza. There was no significant difference in the expression pattern between A. corniculatum and B. gymnorrhiza. The research aims to better understand the physiological mechanism of mangrove to resist waterlogging stress and to provide theoretical foundation for protecting mangrove ecosystem.

Key words: mangrove plants, water-logging stress, antioxidant enzyme system, clustering analysis

中图分类号:

Q945.78

苏柏予, 张维仕, 王友绍. 三种红树根部组织抗氧化酶活性对水淹胁迫的响应[J]. 热带海洋学报, 2022, 41(6): 35-43.

SU Boyu, ZHANG Weishi, WANG Youshao. Response of antioxidant enzyme systems in root tissues of three mangrove species to waterlogging stress[J]. Journal of Tropical Oceanography, 2022, 41(6): 35-43.

图/表 6

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三种红树根部组织抗氧化酶活性对水淹胁迫的响应

Response of antioxidant enzyme systems in root tissues of three mangrove species to waterlogging stress

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