中国南海来源真菌Penicillium sp. SCSIO 40438次级代谢产物研究
朱义广(1978—), 男, 山东省济宁市人, 研究员, 从事天然产物生物合成研究。email: |
Copy editor: 姚衍桃
收稿日期: 2022-04-14
修回日期: 2022-05-08
网络出版日期: 2022-05-10
基金资助
广东省自然资源厅项目(粤自然资合[2021]48号)
中国科学院王宽诚率先人才计划“卢嘉锡国际团队项目”(GJTD-2020-12)
广东省重点领域研发计划项目(2020B1111030005)
广东省科学技术厅项目(2019TQ05H299)
Study on the secondary metabolites of fungus Penicillium sp. SCSIO 40438 from the South China Sea
Copy editor: YAO Yantao
Received date: 2022-04-14
Revised date: 2022-05-08
Online published: 2022-05-10
Supported by
Guangdong MEPP Funds(GDNRC[2021]48)
K.C.Wong Education Foundation(GJTD-2020-12)
Key Area Research and Development Program of Guangdong Province(2020B1111030005)
Special Support Program for Training High Level Talents in Guangdong(2019TQ05H299)
本论文对中国南海来源真菌Penicillium sp. SCSIO 40438的次级代谢产物进行了研究。通过硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及高效液相色谱等多种分离方法对该菌株的发酵产物进行了分离纯化, 并利用核磁共振和高分辨质谱等波谱学方法对分离得到的化合物进行了结构鉴定。从中共分离鉴定到9个化合物: 1-(2-Methylbut-3-en-2-yl)-1H-indole-3-carbaldehyde (1)、1-methyl-2(1H)-quinazolinone (2)、fructigenine A (3)、fructigenine B (4)、2-[(s)-hydroxy(phenyl)methyl]-3-methylquinazolin-4(3H)-one (5)、3-methylviridicatin (6)、3-O-methylviridicatol (7)、viridicatol (8)和(+)-cyclopenol (9)。其中, 化合物1和2为新天然产物。抑菌活性实验表明, 化合物8具有中等强度的抑制金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌活性, 最低抑菌浓度为8.0µg·mL-1。
关键词: 真菌; Penicillium sp.; 次级代谢产物; 抑菌活性
朱义广 , 牟鹏云 , 张庆波 , 张长生 . 中国南海来源真菌Penicillium sp. SCSIO 40438次级代谢产物研究[J]. 热带海洋学报, 2023 , 42(1) : 161 -167 . DOI: 10.11978/2022079
This experiment aims to study the secondary metabolites of fungus Penicillium sp. SCSIO 40438 from the South China Sea. The solid fermentation products of the strain were isolated and purified by a variety of separation methods, including silica gel column, Sephadex LH-20 gel column, and preparative high performance liquid chromatography. The structures of the isolated compounds were identified by nuclear magnetic resonance, high resolution electrospray ionization mass spectroscopy and comparison with the previously reported data. Nine compounds were obtained and identified as 1-(2-Methylbut-3-en-2-yl)-1H-indole-3-carbaldehyde (1), 1-methyl-2(1H)-quinazolinone (2), fructigenine A (3), fructigenine B (4), 2-[(s)-hydroxy(phenyl)methyl]-3-methylquinazolin-4(3H)-one (5), 3-Methylviridicatin (6), 3-O-methylviridicatol (7), viridicatol (8), (+)-cyclopenol (9), and compounds 1 and 2 are two new natural products. Compound 8showed moderate antibacterial activities against Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus with minimal inhibition concentration (MIC) values of 8.0 μg·mL-1.
Keywords fungi; Penicillium sp.; secondary metabolites; antibacterial activity
表1 化合物1—9的抑菌活性测试结果Tab. 1 The antibacterial activities of compounds 1~9 |
化合物 | M. Luteus SCSIO ML01 | S. Aureus ATCC 29213 | MRSA ATCC 43300 | P. aeruginosa ATCC 27853 | A. baumannii ATCC 19606 | E.coli ATCC 25922 |
---|---|---|---|---|---|---|
1 | 32.0 | >64.0 | 32.0 | >64.0 | >64.0 | >64.0 |
2 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
3 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
4 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
5 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
6 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
7 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
8 | >64.0 | 8.0 | 8.0 | >64.0 | >64.0 | >64.0 |
9 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
对照 | 0.5a | 0.5a | 0.5a | 1.0b | 1.0b | 1.0b |
注:a为万古霉素, b为环丙沙星 |
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