为了研究阿拉伯宝螺(Mauritia arabica)来源的真菌细菌共生体Aspergillus spelaeus GXIMD 04541/Sphingomonas echinoides GXIMD 04532的次级代谢产物, 利用硅胶柱层析、凝胶柱层析以及半制备高效液相色谱(HPLC)对菌株发酵提取物进行分离纯化, 运用现代波谱学方法并结合相关文献数据比对, 鉴定化合物结构, 通过MTT法评价化合物细胞毒活性。结果表明从共生体GXIMD 04541/04532的大米发酵提取物中分离得到5个细胞松弛素类化合物和2个苯内酯类化合物, 分别为trichalasins C (1)、aspochalasin E (2)、aspochalasin H (3)、aspochalasin I (4)、aspochalasin K (5)、citreofuran (6)、cyclothiocurvularin B methyl ester (7)。活性测试结果显示, 化合物2、4和5表现出PC3细胞毒活性, IC50分别为17.23, 15.18和8.71 μM, 化合物1-5未表现出22Rv1细胞毒性。本研究丰富了海洋真菌-细菌共生体来源的代谢产物化学多样性。

In order to investigate the secondary metabolites of the fungal-bacterial symbiont Aspergillus spelaeus GXIMD 04541/Sphingomonas echinoides GXIMD 04532 derived from Mauritia Arabica, compounds were isolated and purified by silica gel chromatography, sephadex LH-20 gel column chromatography, and semi-preparative high performance liquid chromatography (HPLC). The structures of the compounds were identified by modern spectroscopic methods and compared with relevant literature data. Cytotoxic activity evaluation of the compounds was conducted using MTT assay. Consequently, seven compounds were obtained from rice fermentation extracts of symbiont GXIMD 04541/04532, which were identified as trichalasins C (1), aspochalasin E (2), aspochalasin H (3), aspochalasin I (4), aspochalasin K (5), citreofuran (6), and cyclothiocurvularin B methyl ester (7). The results of the activity tests demonstrated that compounds 2, 4 and 5 exhibited cytotoxic activity against PC3 cells, with IC50 values of 17.23, 15.18 and 8.71 μM, respectively. Howerver, compounds 1-5 didn’t show cytotoxicity against 22Rv1 cells. This study enrichs the chemical diversity in metabolites derived from marine fungal-bacterial symbiont sources.