为获得无瓣海桑果实中具有延缓衰老活性的化合物, 对其正丁醇部位开展成分分离及活性研究。运用反相硅胶色谱、凝胶色谱、高压液相色谱手段对其进行分离纯化, 通过波谱分析及文献对照鉴定结构。以寿命、热应激、咽泵频率、弯曲能力、脂褐素为指标, 考察化合物延缓秀丽隐杆线虫衰老活性, 并通过分子对接预测化合物与DAF-16蛋白的作用方式。从正丁醇部位首次分离得到5个化合物, 鉴定为1, 2, 3, 6-四-O-没食子酰基-β-D-吡喃葡萄糖 (1)、hovetrichoside C (2)、(6R,9S)-3-氧代-α-紫罗兰醇-β-D-葡萄糖苷 (3)、山柰酚-3-O-β-D葡萄糖苷 (4)、3-甲基丁基β-D-吡喃葡萄糖 (5)。化合物2、4、5在100μmol·L-1浓度下具有显著的延缓衰老活性, 分别延长秀丽隐杆线虫寿命的15.9%、15.0%、22.1%, 极显著延长热应激条件下线虫寿命的36.3%、50.6%、46.3%。可提升线虫的咽泵频次、身体弯曲能力以及降低脂褐素的累积。分子对接表明, 化合物2、4、5能与DAF-16蛋白氨基酸稳定结合, 结合能分别为-6.6kcal·moL-1、-6.0kcal·moL-1、-4.6kcal·moL-1。本研究丰富了无瓣海桑果实中延缓衰老的活性物质基础, 为无瓣海桑果实的开发利用提供理论依据。
刘建雲
,
夏家朗
,
白猛
,
李玥瑶
,
韦金花
,
刘锴
,
高程海
,
易湘茜
. 红树无瓣海桑果实中糖苷鉴定及其延缓线虫衰老活性研究[J]. 热带海洋学报, 0
: 1
.
DOI: 10.11978/2024150
In order to obtain compounds with anti-aging activity in the fruit of Sonneratia apetala, the chemical constituents of n-butanol alcohol extract were separated and their bioactivities were studied. Compounds were isolated and purified by reverse silica column, gel chromatography and high pressure liquid chromatography. The structure of compounds was identified by modern spectral analysis and literature contrast. The anti-aging activity of compounds were investigated based on Caenorhabditis elegans used the lifespan, heat stress, pharyngeal pumping capacity, body bending ability and lipofuscin as indicator. Molecular docking was used in predicted the interacted mode of compounds with protein DAF-16. Five compounds were isolated from the n-butanol alcohol extract for the first time, identified as 1, 2, 3, 6-tetra-O-galloyl -β-D-glucopyranose (1), hovetrichoside C (2), (6R, 9S)-3-oxo-α-ionol-β-D-glucoside (3), kaempferol-3-O-β-D glucoside (4), 3-methylbutyl β-D-glucopyranoside (5). Compounds 2, 4 and 5 showed an anti-aging activity at 100μmol·L-1 concentration significantly, extended the lifespan of C. elegans in normal culture by 15.9%, 15.0% and 22.1% respectively, significantly prolong the lifespan under heat stress by 36.3%, 50.6% and 46.3% respectively. Improved the frequency of pharyngeal pumping, ability of body bending, and reduced lipofuscin accumulated. Molecular docking showed that compounds 2, 4 and 5 can stably bind to the amino acids of DAF‐16 protein. The binding energy were -6.6kcal·moL-1, -6.0kcal·moL-1, -4.6kcal·moL-1 respectively. This study enriched the material basis of anti-aging activity and provided a basis for the development and utilization in the Sonneratia apetala fruit.
