三种红树根部组织抗氧化酶活性对水淹胁迫的响应
苏柏予(1997—), 女, 广西梧州市人, 硕士研究生, 从事红树林生态学研究。email: |
Copy editor: 林强
收稿日期: 2021-08-21
修回日期: 2021-10-12
网络出版日期: 2021-10-14
基金资助
国家重点研发计划项目(国家科技基础资源调查专项)(2017FY100700)
国家自然科学基金重点项目(U1901211)
国家自然科学基金重点项目(41876126)
中国科学院A类战略性先导科技专项(XDA23050200)
国际伙伴计划(133244KYSB20180012)
南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0305)
Response of antioxidant enzyme systems in root tissues of three mangrove species to waterlogging stress
Copy editor: LIN Qiang
Received date: 2021-08-21
Revised date: 2021-10-12
Online published: 2021-10-14
Supported by
National Key Research and Development Program of China(2017FY100700)
National Natural Science Foundation of China(U1901211)
National Natural Science Foundation of China(41876126)
Strategic Priority Research Program of the Chinese Academy of Sciences(XDA23050200)
International Partnership Program of Chinese Academy of Sciences(133244KYSB20180012)
Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0305)
以自然种群高程分布为根据, 选择对潮汐浸泡耐性存在一定差异的白骨壤(Avicennia marina)、桐花树(Aegiceras corniculatum)和木榄(Bruguiera gymnorrhiza)为实验树种。对3种红树幼苗进行为期0d、1d、3d、5d、7d、14d的水淹处理, 测定其根部组织的过氧化物酶(peroxidase, POD)活性、超氧化物歧化酶(superoxide dismutase, SOD)活性、抗坏血酸氧化酶(ascorbic acid oxidase, APX)活性和过氧化氢酶(catalase, CAT)活性。水淹处理会导致植物根部组织受低氧胁迫从而破坏活性氧(reactive oxygen species, ROS)生产和消除的平衡, ROS大量累积给植物带来氧化损伤。实验测定与清除ROS有关的酶指标, 结果表明POD活性在1d至14d的水淹周期内随时间呈现上升趋势, 这可能与POD参与活性氧以及乙醇、乳酸等有毒物质的反应有关。CAT、SOD、APX活性均呈现应激上升后下降趋势, 由此推断该系列抗氧化酶在水淹胁迫发生前中期对消除红树植物根部组织大量产生的活性氧具有重要意义。抗氧化酶系在3种红树植物中的表达存在一定差异, 但整体仍呈相似的变化趋势。通过层次聚类法对各项酶活性指标分析后发现, 白骨壤抗氧化酶系的活性模式与木榄和桐花树的存在一定差异, 但没有发现木榄与桐花树抗氧化酶系统表达模式的明显差异。本研究旨在更好地了解红树林对水淹胁迫的生理适应机制, 为红树林生态系统保护与管理提供理论依据。
苏柏予 , 张维仕 , 王友绍 . 三种红树根部组织抗氧化酶活性对水淹胁迫的响应[J]. 热带海洋学报, 2022 , 41(6) : 35 -43 . DOI: 10.11978/2021107
Based on the natural distribution of different mangrove populations, Avicennia marina, Aegiceras corniculatum and Bruguiera gymnorrhiza were chosen to be the experimental species that have different resistance of water-logging. The activities of peroxidase (POD), superoxide dismutase (SOD), ascorbate oxidase (APX) and catalase (CAT) in root tissues of three species of mangrove were tested after submerging for 0d, 1d, 3d, 5d, 7d and 14d. Under hypoxia environment, the balances of production and elimination of reactive oxygen species (ROS) would be destroyed and a large amount of ROS were accumulated. The results showed that POD increased with time during the period from 1d to 14d, which may be related to the involvement of scavenging of toxic substances in addition to the elimination of reactive oxygen. The CAT, SOD and APX increased when mangroves encounter the water-logging and then decreased. The enzymes play an important role in eliminating the large amount of ROS in the root tissues of mangrove plants during the early period of the waterlogging stress. There were some differences in the expression quantity of antioxidant enzyme system among the three mangrove plants but the fluctuations are similar. Hierarchical clustering analysis of various enzyme activity indexes indicated that the activity pattern of antioxidant enzyme system in A.marina was different from that in A. corniculatum and B. gymnorrhiza. There was no significant difference in the expression pattern between A. corniculatum and B. gymnorrhiza. The research aims to better understand the physiological mechanism of mangrove to resist waterlogging stress and to provide theoretical foundation for protecting mangrove ecosystem.
图6 酶活性水平聚类分析树状图各分支标签中am为白骨壤, ac为桐花树, bg为木榄; 标签数字尾缀代表水淹处理天数 Fig. 6 Dendrogram derived from cluster analysis of enzymes activities. In the labels of each branch, am represents A. marina, ac stands for A. corniculatum and bg means B. gymnorrhiza. The label number represents the periods of submerged stress |
[1] |
白果, 冷平生, 胡增辉, 2021. 水淹胁迫对4年生华北紫丁香生理指标的影响[J]. 西部林业科学, 50(4): 101-106.
|
[2] |
崔雪, 饶良懿, 周其令, 2018. 水淹胁迫下饲料桑苗(Morus alba)叶片形态及部分酶活性的变化[J]. 生态学杂志, 37(12): 3633-3639.
|
[3] |
黄雪松, 陈燕丽, 莫伟华, 等, 2021. 近60年广西北部湾红树林生态区气候变化及其影响因素[J]. 生态学报, 41(12): 5026-5033.
|
[4] |
金艺, 李旭, 傅松玲, 等, 2021. 水淹胁迫下的水竹根系生理响应与适应特征[J]. 安徽农业大学学报, 48(1): 1-8.
|
[5] |
梁芳, 檀小辉, 邓旭, 等, 2021. 半红树植物玉蕊对淹水-盐度胁迫的生长及生理响应[J]. 广西植物, 41(6): 872-882.
|
[6] |
廖宝文, 邱凤英, 张留恩, 等, 2010. 红树植物白骨壤小苗对模拟潮汐淹浸时间的生长适应性[J]. 环境科学, 31(5): 1345-1351.
|
[7] |
林鹏, 2001. 中国红树林研究进展[J]. 厦门大学学报(自然科学版), 40(2): 592-603.
|
[8] |
刘锦, 2018. 红树植物对高温响应与适应的生理生化特征及其分子生态学机制[D]. 广州: 中国科学院大学.
|
[9] |
卢昌义, 林鹏, 叶勇, 等, 1995. 全球气候变化对红树林生态系统的影响与研究对策[J]. 地球科学进展, 10(4): 341-347.
|
[10] |
谭淑端, 朱明勇, 张克荣, 等, 2009. 植物对水淹胁迫的响应与适应[J]. 生态学杂志, 28(9): 1871-1877.
|
[11] |
谭淑端, 朱明勇, 张克荣, 等, 2013. 水淹对双穗雀稗抗氧化酶活性及碳水化合物含量的影响[J]. 草业学报, 22(1): 217-224.
|
[12] |
王友绍, 2019. 红树林分子生态学[M]. 北京: 科学出版社: 23-24.
|
[13] |
王友绍, 2021. 全球气候变化对红树林生态系统的影响、挑战与机遇[J]. 热带海洋学报, 40(3): 1-14.
|
[14] |
温远光, 刘世荣, 元昌安, 2002. 广西英罗港红树植物种群的分布[J]. 生态学报, 22(7): 1160-1165.
|
[15] |
夏斌, 刘莹, 胡尚春, 等, 2019. 水淹胁迫对虎尾草生理指标的影响[J]. 东北林业大学学报, 47(7): 31-36.
|
[16] |
颜秀花, 蔡榕硕, 郭海峡, 等, 2019. 气候变化背景下海南东寨港红树林生态系统的脆弱性评估[J]. 应用海洋学学报, 38(3): 338-349.
|
[17] |
杨玲, 刘玲, 胡馨月, 等, 2020. 水淹胁迫对小蓬草(Conyza canadensis)的形态结构与生理生化特性的影响[J]. 生态科学, 39(5): 134-141.
|
[18] |
张梦如, 杨玉梅, 成蕴秀, 等, 2014. 植物活性氧的产生及其作用和危害[J]. 西北植物学报, 34(9): 1916-1926.
|
[19] |
张以科, 2008. 广东红树林湿地净化石油和多环芳烃类污染物的功能及其价值估算[D]. 广州: 华南师范大学.
|
[20] |
赵楚, 张岳, 辛建攀, 等, 2021. 水分胁迫对棱角山矾生长和生理的影响[J]. 东北林业大学学报, 49(5): 12-18.
|
[21] |
|
[22] |
|
[23] |
|
[24] |
|
[25] |
|
[26] |
|
[27] |
|
[28] |
|
[29] |
|
[30] |
|
[31] |
|
[32] |
|
[33] |
|
[34] |
|
[35] |
|
/
〈 |
|
〉 |