收稿日期: 2008-04-10
修回日期: 2008-06-06
网络出版日期: 2010-07-29
基金资助
广东省自然科学基金博士启动项目(06301430); 国家自然科学基金(30800032)
Biodegradation of dimethyl phthalate by Burkholderia sp. DA2 isolated from marine sediments in the South China Sea
Received date: 2008-04-10
Revised date: 2008-06-06
Online published: 2010-07-29
Supported by
广东省自然科学基金博士启动项目(06301430); 国家自然科学基金(30800032)
以邻苯二甲酸二甲酯(dimethyl phthalate, DMP)为惟一碳源, 从中国南海底泥中分离筛选到一株高效降解菌株, 即伯克霍尔德氏菌Bukholderia. sp. DA2。该菌株降解DMP及其邻苯二甲酸二甲酯(Dimethyl phthalate, DMT)和间苯二甲酸二甲酯(Dimethyl dimethyl isophthalate, DMI) 2种异构体的效果显著。B. sp. DA2菌株能够在8d内好氧降解矿化100mg·L-1的DMT, 15d内好氧降解矿化400mg·L-1的DMP。但对于200mg·L-1 的DMI, 则不能彻底降解矿化, 反应终止在生成间苯二甲酸一甲酯中间产物的阶段。研究表明, B. sp.DA2菌株降解DMT及DMP的途径相似, 首先脱酯生成单酯和甲醇, 继而水解酸化, 直至彻底矿化。降解速率及其途径, 与化学结构密切相关。
王亚丽,殷波,洪义国,顾继东 . 中国南海Bukholderia sp. DA2菌株降解邻苯二甲酸二甲酯研究[J]. 热带海洋学报, 2010 , 29(4) : 71 -75 . DOI: 10.11978/j.issn.1009-5470.2010.04.071
Burkholderia sp. DA2 strain isolated from marine sediments in the South China Sea was capable of utilizing dimethyl phthalate (DMP) as the sole source of carbon and energy for biodegradation of DMP, DMT (dimethyl phthalate) and DMI (dimethyl isophthalate). During the transformation of DMP (400mg·L-1), its corresponding intermediates were identified as mono-methyl phthalate (MMP) and phthalate acid (PA) by HPLC analysis. By the 9th day, the DMP concentration was undetectable in the culture, corresponding to a DMP decrease; MMP accumulated to 38.9 mg/L during eight days, at the same time amount of PA was also produced during the first 12 days of degradation; this PA was completely degraded after 15 days. During the transformation of DMT (100mg·L-1), 80% DMT was transferred into MMT in one day, but the MMT could not degraded rapidly and accumulated to 80.2mg·L-1, and completely mineralized during eight days. During the DMI (200mg·L-1) degradation, the DMI was transferred into MMI after six days, but the MMI could not further mineralize. Maybe the compounds chemical structures infect the activity of the enzyme degradation. The biodegradation pathway of DMP, DMT and DMI was proposed as DMP\DMT\DMI→MMP\MMT\MMI→PA\TA\IA→ ... →CO2+H2O.
Key words： DMP; DMI; DMT; biodegradation; marine microorganism
[1] ATLAS E, GIAM C S. Global transport of organic pollutants: Ambient concentrations in the remote marine atmosphere[J]. Science, 1981, 211: 163-165.
[2] FAJARDO C, GUYOT J P, MACARIE H, et al. Inhibition of anaerobicdigestion by terephthalic acid and its aromatic by products[J]. Water Sci Technol, 1997, 36: 83-90.
[3] SUGIURA K, SCHMID A F, SCHMID M M, et al. Effect of cornDounds on a spectrum of rat tumors[J]. Cancer Chemother Rep. Part. 2, 1973, 3(1): 231-308.
[4] SCHWARZENBACH R P, ESCHER B I, FENNER K, et al. The challenge of micropollutants in aquatic systems[J]. Science, 2006, 313: 1072-1077.
[5] ZHAO X K, YANG G P, WANG Y J. Adsorption of dimethyl phthalate on marine sediments[J]. Water Air Soil Pollut, 2004, 57: 179-192.
[6] GIAM, C S, ATLAS E, POWERS MA, et al. Phthalic acid esters. In: Handbook of Environmental Chemistry[J]. Anthropogenic Compounds, 1984, 3: 67-142.
[7] STAPLES C A, PETERSON D R, PARKERTON T F, et al. The environmental fate of phthalate esters: aliterature review[J]. Chemosphere, 1997, 35: 667-749.
[8] WANG Y, GU J-D. Degradation of dimethyl terephthalate by Variovorax paradoxus T4 and Sphingomonas paucimobilis DOS1 of the South China Sea
[J]. Ecotoxicology, 2006, 15: 549-57.
[9] WANG Y, GU J-D. Degradation of dimethyl isophthalate by Viarovorax paradoxus T4 isolated from deep-ocean sediment of South China Sea[J]. J Human Ecol Risk Assess, 2006, 12: 236-47.
[10] HAIGLER B E, PETTIGREW C A, SPAIN J C. Biodegradation of mixtures of substituted benzenes by Pseudomonas sp. strain JS150[J]. Appl Environ Microbiol, 1992, 58: 2237-2244.
[11] JOHNSON G R, OLSEN R H. Multiple pathways for toluene degradation in Burkholderia sp. strain JS150[J]. Appl Environ Microbiol, 1997, 63: 4047-4052.
[12] CHANG H, ZYLSTRA G J. Characterization of the phthalate permease OphD from Burkholderia cepacia ATCC 17616[J]. Journal of Bacteriology, 1999, 181: 6197-6199.
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