收稿日期: 2008-04-02
修回日期: 2008-05-28
网络出版日期: 2010-07-29
基金资助
广东省中国科学院战略合作项目(2009B091300156); 广东省科技计划项目(2006B20201054); 广东省海洋渔业科技专项(A2009004-004)
Breeding and miniaturizing screening of rotifer Brachionus plicatilis by applying UV radiation and mutagenic agents
Received date: 2008-04-02
Revised date: 2008-05-28
Online published: 2010-07-29
Supported by
广东省中国科学院战略合作项目(2009B091300156); 广东省科技计划项目(2006B20201054); 广东省海洋渔业科技专项(A2009004-004)
运用物理方法紫外线辐射(UV)和化学诱变剂秋水仙素、甲基磺酸乙酯(EMS)对褶皱臂尾轮虫Brachionus plicatilis进行诱变处理及小型化轮虫选育。结果表明, 随着UV辐射剂量和化学诱变剂浓度的增加, 轮虫的孵 化率、挂卵率呈下降趋势。辐射剂量为4.8´104—6.0´104μW·s·cm-2时的孵化率、挂卵率指数减小; 1.2´105— 1.5´105μW·s·cm-2后减小趋势变缓。对照组轮虫平均体长250μm, UV辐射后轮虫平均体长200μm; 秋水仙素20mg·L-1组的轮虫长、短轴、卵经相对对照组分别减少20%、14%、7.7%; 甲基磺酸乙酯10%(体积分数)组减少30%、19%、12%。实验得到最佳UV辐射剂量和化学诱变剂浓度: 辐射强度400μW·cm-2, 辐射时间为2min, 最终辐射剂量达到5.0´ 104μW·s·cm-2; 秋水仙素浓度2mg·L-1、甲基磺酸乙酯浓度为0.6%(体积分数), 浸渍时间为12h。
关键词： 褶皱臂尾轮虫Brachionus plicatilis; 紫外线辐射; 秋水仙素; 甲基磺酸乙酯; 小型化筛选
郭峰,刘学东,蔡泽平,林岳光,张俊彬 . UV辐射及化学诱变剂对褶皱臂尾轮虫的诱变选育[J]. 热带海洋学报, 2010 , 29(4) : 76 -81 . DOI: 10.11978/j.issn.1009-5470.2010.04.076
Ultraviolet (UV) radiation and mutagenic agents, including colchicine and ethyl methane sulfonate (EMS), are applied in the mutation breeding and miniaturizing screening of Brachionus plicatilis. B. plicatilis shows a decrease in incubation rate, oocyte production rate and individual sizes with the rising of UV radiation and the densities of mutagenic agents. When the UV radiation reaches 4.8´104-6.0´104μW·s·cm-2, there is a distinct and rapid decline of the incubation rate. When the UV radiation reaches1.2´105-1.5´105μW·s·cm-2, both rates start to decline more slowly. The average body length of rotifers in the group treated with UV is 200nm, which is 50nm shorter than the control group. Length in rotifer long axis, short axis and oocyte axis decrease by 20%, 14% and 7.7% in the group treated with 20mg·L-1 colchicine and by and 30%, 19% and 12%, with 10% (volume percentage concentrations) EMS, respectively. The best radiation dose and the density of the mutagenic agents are radiant intensity 400μW·cm-2, radiant time 2 minutes, and final radiation dose 5.0´104μW·s·cm-2. The optimal densities of the mutagenic agents are colchicines of 2mg·L-1, EMS of 0.6% (volume percentage concentrations), and time of steeping over 12 hours.
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