收稿日期: 2009-11-15
修回日期: 2010-06-04
网络出版日期: 2010-12-15
基金资助
国家“863”计划子课题(2006AA10A406, 2010AA10A401); 国家科技支撑计划子课题(2006BAD01A1303); 广东省海洋渔
业科技推广专项(A200899A02)
A diallel analysis on growth traits in shrimp Litopenaeus vannamei
Received date: 2009-11-15
Revised date: 2010-06-04
Online published: 2010-12-15
Supported by
国家“863”计划子课题(2006AA10A406, 2010AA10A401); 国家科技支撑计划子课题(2006BAD01A1303); 广东省海洋渔
业科技推广专项(A200899A02)
以来源于两个不同遗传背景群体的凡纳滨对虾Litopenaeus vannamei为亲本, 设置PP(P♀×P♂)、 PH(P♀×H♂)、HP(H♀×P♂)、HH(H♀×H♂) 4个实验组(P代表进口群体, H代表选育群体), 进行进口个体(P♂和P♀)与选育个体(H♂和H♀)间的双列杂交实验, 分析比较其子一代的生长与存活情况。结果发现, 对虾存活由高到低顺序为HH>HP>PH>PP, PH实验组表现出明显的存活杂种优势, 其育苗期间的杂种优势平均值为(27.72?11.88)%, 下塘90d后成活率的杂种优势为30.50%。在生长前期(30日龄以前)对虾平均体长和体重的顺序是HP>PH>HH>PP, 而在生长后期则为PP>HP>PH>HH。HP实验组在整个养成期(10、30、60和90d)均表现出体长和体重上的杂种优势, 杂种优势值分别为24.00%、13.71%、2.55%、2.79%和112.46%、54.06%、8.16%、12.48%。结果表明, 选育群体为母本、进口群体为父本的对虾杂交后代的生长和存活性状明显提高。
林红军,沈琪,张吕平,胡超群,梁立清 . 凡纳滨对虾生长性状的双列杂交分析[J]. 热带海洋学报, 2010 , 29(6) : 51 -56 . DOI: 10.11978/j.issn.1009-5470.2010.06.051
The authors analyzed the growth and survival of the first generation of Litopenaeus vannamei after crossing within and between imported shrimp individuals (P♂, P♀) and selected individuals (H♂, H♀) using complete diallel crosses to set up four combinations, PP(P♀×P♂), PH(P♀×H♂), HP(H♀×P♂) and HH(H♀×H♂). The results showed that survival rate was in the order of HH>HP>PH>PP, and the survival heterosis appeared in PH, with average survival heterosis of (27.72?11.88)% in the young stage and 30.50 at the 90th day. In the early growth stage (the first 30 days), the average body length and weight were both sequenced as HP> PH> HH> PP, while in the late growth, as PP> HP> PH> HH. The growth heterosis was found in body length and weight. The values of body length heterosis were 24.00%, 13.71%, 2.55%, and 2.79%; while the values of body weight heterosis were 112.46%, 54.06%, 8.16%, and 12.48% at the 10th, 30th, 60th and 90th days. In conclusion, the growth and survival traits of hybrids offspring crossing selection individuals as male parents and imported individuals as female parents will be improved significantly.
Key words： Litopenaeus vannamei; diallel cross; heterosis; growth; survival rate
[1] 楼允东. 鱼类育种学[M]. 北京: 中国农业出版社, 1998: 23.
[2] 沈俊宝, 刘明华. 鱼类育种学[M]. 北京: 中国农业出版 社, 1999: 10-39.
[3] SHERIDAN A K. Genetic improvement of oyster production-a critique[J]. Aquac, 1997, 153(3/4): 165-179.
[4] CRUZ P, IBARRA M. Larval growth and survival of two catarina scallop (Argopecten circularis, Sowerby, 1835) populations and their reciprocal crosses[J]. J Exp Mar Bio Ecol, 1997, 212(1): 95-110.
[5] THANH N M, PONZONI R W, NGUYER N H, et al. Evaluation of growth performance in a diallel cross of three strains of giant freshwater prawn (Macrobrachium rosenbergii) in Vietnam[J]. Aquac, 2009, 287(1/2): 75-83.
[6] 姚雪梅, 黄勃, 赖秋明, 等. 凡纳滨对虾自交系与杂交系早期生长和存活的比较[J]. 水产学报, 2006, 30(6): 792-795.
[7] 田燚, 孔杰, 杨翠华. 中国对虾2个群体杂交子一代生长和存活率比较[J]. 科学通报, 2006, 51(15): 1771-1774.
[8] 张吕平, 吴立峰, 沈琪, 等. 凡纳滨对虾全同胞家系的建立及生长比较[J]. 水产学报, 2009, 33(6): 942-949.
[9] 赖秋明, 王海石. 凡纳滨对虾人工授精的初步研究[J]. 海洋科学, 2005, 29(10): 19-22.
[10] GOYARD E, PATROIS J, PEIGNON J M. Selection for better growth of Penaeus stylisrostris in Tahiti and
[11] 童馨, 龚世圆. 凡纳滨对虾不同世代养殖群体的遗传多样性分析[J]. 海洋与湖沼, 2009, 40(2): 214-220.
[12] 沈琪, 任春华, 胡超群, 等. 凡纳滨对虾优良性状的遗传标记筛选[J]. 海洋科学集刊, 2002, 44: 134-138.
[13] 王亚馥, 戴灼华. 遗传学[M].北京:高等教育出版社, 1999: 528-536.
[14] HAYMAN B I. The theory and analysis of diallel crosses[J]. Genetics, 1954, 39(6): 789-809.
/
| 〈 |
|
〉 |
