收稿日期: 2009-04-13
修回日期: 2010-05-04
网络出版日期: 2011-03-16
基金资助
中科院重要方向性项目(KSCX2-YW-R-093); 海洋公益性行业科研专项经费项目(200705026)
Influence of trace elements, Fe, Mn, and Co, on growth and toxin-producing of toxic marine dinoflagellate Coolia monotis
Received date: 2009-04-13
Revised date: 2010-05-04
Online published: 2011-03-16
Supported by
中科院重要方向性项目(KSCX2-YW-R-093); 海洋公益性行业科研专项经费项目(200705026)
实验通过培养基筛选实验和在此基础上的微量元素Fe、Mn、Co正交实验对海南三亚热带珊瑚礁海域有毒甲藻Coolia monotis的生长和产毒影响进行了初步研究, 以期获得充足的原材料进一步开展毒素化合物的研究。实验结果表明, 在Erd、f/2、GP和K 4种培养配方中, K系列培养液更适合于C. monotis的培养。在以2K为基础培养配方的微量元素正交实验中, Fe、Mn和Co 3种微量元素的浓度配比为: Fe: 2?10?5mol•L?1, Co: 1?10?6mol•L?1, Mn: 5?10?7mol•L?1时能获得最大的平均生长速率; 而Fe: 5?10?6mol•L?1, Co: 1?10?7mol•L?1, Mn: 2?10?6mol•L?1的条件下C. monotis的毒性最强。
关键词: 甲藻门; Coolia monotis; 微量元素; 生长速率; 毒性实验
梁计林,龙丽娟,张偲,吴军 . 微量元素Fe、Mn、Co对有毒甲藻生长和产毒的影响[J]. 热带海洋学报, 2011 , 30(1) : 119 -123 . DOI: 10.11978/j.issn.1009-5470.2011.01.119
The optimal media was selected by a media choosing experiment on growth of toxic marine dinoflagellate Coolia monotis isolated from tropic coral reef waters around Sanya, Hainan Island. Based on the result, the influence of different concentrations of three trace elements, Fe, Mn, and Co, on growth and toxin-producing of C. monotis were studied by an or-thogonal experiment. Our results show that the optimal media is K series among Erd, f/2, GP, and K media. The maximum average growth rate exhibits under the concentrations of 2?10?5mol•L?1, 1?10?6mol•L?1, and 5?10?7mol•L?1 for Fe, Co, and Mn, respectively. When concentrations were 5?10?6mol•L?1 for Fe, 1?10?7mol•L?1 for Co, and 2?10?6mol•L?1 for Mn, C. monotis presented the strongest toxicity.
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