收稿日期: 2010-07-26
修回日期: 2010-09-15
网络出版日期: 2011-05-04
基金资助
国家高技术研究发展计划项目(2008AA09Z408); 国家自然科学基金项目(40930847、40876061); 滨海湿地生态系统教育部重点实验室开放基金项目(CWe0902); 福建省自然科学基金项目(2010J01223); 福建省教育厅项目(JA10232)
Optimization of medium components and culture conditions of algicidal actinomy-cetes BS01
Received date: 2010-07-26
Revised date: 2010-09-15
Online published: 2011-05-04
Supported by
国家高技术研究发展计划项目(2008AA09Z408); 国家自然科学基金项目(40930847、40876061); 滨海湿地生态系统教育部重点实验室开放基金项目(CWe0902); 福建省自然科学基金项目(2010J01223); 福建省教育厅项目(JA10232)
从漳江口红树林区采集的沉积物样品中分离到1株放线菌菌株BS01 Brevibacterium sp., 其胞外活性产物对塔玛亚历山大藻Alexandrium tamarense具有明显的溶藻作用。采用单因素及均匀设计, 通过摇瓶培养对BS01产溶藻活性物质的发酵培养基及发酵条件进行优化。结果表明, 在可溶性淀粉为碳源、硝酸钠为氮源、装液量为40%、起始pH值为7.5、培养温度为28℃、转速为150r•min-1、振荡培养时间为48h的条件时, BS01发酵产物的杀藻活性最强。通过均匀设计进行最佳发酵培养基及培养条件优化的结果为: 可溶性淀粉为20g•L-1，硝酸钠为0.5g•L-1，pH为7.7，温度为27.2℃。研究结果为杀藻活性物质高效提取及杀藻机制研究奠定了基础。
傅丽君,安新丽,李东,许丽霞,田蕴,郑天凌, . 高效溶藻放线菌BS01发酵培养基及发酵条件优化[J]. 热带海洋学报, 2011 , 30(2) : 109 -114 . DOI: 10.11978/j.issn.1009-5470.2011.02.109
A strain of actinomycetes BS01 capable of lysing the toxic dinoflagellate Alexandrium tamarense was isolated from mangrove area of Zhangjiangkou in Fujian province. The medium components and cultural conditions for algicidal activity of actinomycete strain BS01 were optimized by single-factor and uniform tests in shake-flask. The initial pH, fer-mentation temperature, fermentation time, and inoculum were optimized through single-factor tests. Results showed that BS01 exhibited the best algicidal activity by using soluble starch as carbon source, sodium nitrate as nitrogen source, initial pH at 7.7, temperature at 27.2℃, and shaking at 150r•min-1 in 100mL of 250mL conical flask for 48h. The optimal fer-mentation parameters of BS01 were: soluble starch 20g•L-1, sodium nitrate 0.5g•L-1, pH value 7.7, and temperature 27.2℃ by using uniform design experiments.
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