海洋生物学

华南沿海4 种主要污损生物幼虫和孢子的采集与培养技术

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  • 1.中国科学院南海海洋研究所, 广东 广州 510301; 2.中国科学院大亚湾海洋生物综合实验站, 广东 深圳 518121; 3.广东海洋大学水产学院, 广东 湛江 524088
严涛(1965—), 男, 上海市人,博士, 从事海洋污损生物与防除研究。E-mail: yantao@scsio.ac.cn

收稿日期: 2010-05-14

  修回日期: 2010-06-30

  网络出版日期: 2011-07-20

基金资助

广东省海洋与渔业科技专项(深水网箱抗附着研究); 国家自然科学基金项目(40706046); 中国科学院南海海洋研究所知识创新工程前沿项目(LYQY200703)

Collection and culture for larvae and spores of four common fouling species in coastal waters of southern China

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  • 1. South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Marine Biology Research Station at Daya Bay, CAS, Shenzhen 518121, China; 3. Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China

Received date: 2010-05-14

  Revised date: 2010-06-30

  Online published: 2011-07-20

摘要

海洋污损生物附着是增加海上作业成本、影响设施安全和缩短其使用年限的重要因素, 掌握海洋污损生物幼虫和孢子的采集与培养技术是开展防除研究工作的基础。网纹藤壶 Balanus reticulatus、缘管浒苔 Enteromorpha linza、石莼 Ulva lactuca 和细基江蓠 Gracilaria tenuistipitata 是华南沿海主要的污损生物种类。研究上述 4 种污损生物幼虫和孢子的采集与培养技术, 结果显示, 网纹藤壶无节幼虫于 30℃黑暗环境中培养, 5d 左右即可发育为金星幼虫, 可将其在 4℃条件下储存备用。强度为 4000lx 的光照射缘管浒苔和石莼, 可刺激其游孢子大量释放; 细基江蓠经阴干处理再浸泡海水, 可诱导其果孢子的放散。每天接受 900—1300lx 光照 12h, 室温(21—26 ) ℃ 下培养 4d, 是观察缘管浒苔和石莼游孢子附着萌发的最佳条件, 而细基江蓠果孢子的附着萌发观察则以室温(18—23 ) ℃ 下培养 2d 为宜。

本文引用格式

严涛 ,谢恩义 ,曹文浩 ,刘姗姗 ,何毛贤 ,董钰 ,严文侠 . 华南沿海4 种主要污损生物幼虫和孢子的采集与培养技术[J]. 热带海洋学报, 2011 , 30(3) : 56 -61 . DOI: 10.11978/j.issn.1009-5470.2011.03.056

Abstract

Biofouling on marine installations is a major factor of increasing operation cost and affecting their safety and ser- vice life. Acorn barnacle Balanus reticulatus, green algae Enteromorpha linza and Ulva lactuca, and red alga Gracilaria tenuistipitata are common fouling species in the coastal waters of southern China. This paper deals with the method for the collection and culture of their larvae and spores. Nauplii of acorn barnacle B. reticulatus was reared in darkness at 30℃ for 5 days, and then metamorphosed to cyprids that could be stored at 4℃. A large number of zoospores released when green algae E. linza and U. lactuca were irradiated at the light intensity of 4000lx. Concerning the red alga G. tenuistipitata, carpospore release was induced after the thalli was treated by shade drying first and then immersed in seawater. The spore germination of the green algae E. linza and U. lactuca was observed after being incubated at a room temperature (21?26℃) with 900?1300lx light intensity on a 12h light and 12h darkness cycle for four days, and red alga G. tenuistipitata was at 18?23℃ for two days.

参考文献

[1] 李静, 严涛, 曹文浩, 等. 近海污损生物生态研究进展[J]. 海洋通报, 2010, 29(1): 113-119.
[2] 严涛, 刘姗姗, 曹文浩. 中国沿海水产设施污损生物特点及防除对策[J]. 海洋通报, 2008, 27(1): 102-110.
[3] 严涛, 曹文浩. 黄、渤海污损生物生态特点及研究展望[J]. 海洋学研究, 2008, 26(3): 107-118.
[4] 刘勐伶, 严涛. 南海污损生物生态研究进展[J]. 海洋通报, 2006, 25(1): 84-91.
[5] 曹文浩, 严涛, 李静, 等. 中国沿海污损性无柄蔓足类生态特点概述[J]. 广西科学院学报, 2010, 26(1): 67-73.
[6] 任先秋, 刘瑞玉. 中国近海的蔓足类I. 藤壶属[G]//海洋科学集刊: 第13集. 北京: 科学出版社, 1978: 119-196.
[7] 严文侠, 陈兴乾. 网纹藤壶的幼虫发育[G]//南海海洋科学集刊: 第1集. 北京: 科学出版社, 1980: 125-134.
[8] 严文侠, 庞景梁, 陈兴乾. 网纹藤壶的附着[G]//南海海洋科学集刊: 第4集. 北京: 科学出版社, 1983: 65-73.
[9] 庞景梁, 杨淼森, 严文侠, 等. 网纹藤壶的幼虫培养[G]//南海海洋科学集刊: 第1集. 北京: 科学出版社, 1980: 119-124.
[10] THIYAGARAJAN V, VENUGOPALAN V P, SUBRAMONIAM T, et al. Rearing of barnacle Balanus reticulatus Utinomi larvae using the diatom Chaetoceros wighami as food[J]. Indian J Mar Sci, 1996, 25(4): 365-367.
[11] RITTSCHOF D, CLARE A S, GERHART D J, et al. Barnacle in vitro assays for biologically active substances: Toxicity and settlement inhibition assays using mass cultured Balanus amphitrite amphitrite Darwin[J]. Biofouling, 1992, 6(2): 115-122.
[12] O’CONNOR N J, RICHARDSON D L. Comparative attachment of barnacle cyprids (Balanus amphitrite Darwin, 1854; B. improvisus Darwin, 1854; B. eburneus Gould, 1841) to polystyrene and glass substrata[J]. J Exp Mar Biol Ecol, 1994, 183(2): 213-225.
[13] SATUITO C G, SHIMIZU K, NATOYAMA K, et al. Age-related settlement success by cyprids of the barnacle Balanus amphitrite, with special reference to consumption of cyprid storage protein[J]. Mar Biol, 1996, 127(1): 125-130.
[14] 冯丹青, 柯才焕, 卢昌义, 等. 白脊藤壶(Balanus albicostatus)应用于天然海洋防污产物筛选模型的研究[J]. 海洋与湖沼, 2008, 39(4): 395-400.
[15] 曾呈奎, 张得瑞, 张峻甫, 等. 中国经济海藻志[M]. 北京: 科学出版社, 1962: 36-50.
[16] 丁怀宇, 马家海, 王晓坤, 等. 缘管浒苔的单性生殖[J]. 上海水产大学学报, 2006, 15(4): 493-496.
[17] 马家海, 嵇嘉民, 徐韧, 等. 长石莼(缘管浒苔)生活史的初步研究[J]. 水产学报, 2009, 33(1): 45-52.
[18] CALLOW M E, CALLOW J A. Primary adhesion of Enteromorpha (Chlorophyta, Ulvalves) propagules: quantitative settlement studies and video microscopy[J]. J Phycol, 1997, 33(6): 938-947.
[19] 李伟新, 朱仲嘉, 刘凤贤. 海藻学概论[M]. 上海: 上海科学技术出版社, 1982: 24-217.
[20] BHATTARAI H D, PAUDEL B, HONG Y K, et al. A simple method to preserve algal spores of Ulva spp. in cold storage with ampicillin[J]. Hydrobiologia, 2007, 592(1): 399-404.
[21] 张义浩, 赵盛龙, 吴常文. 海洋生物—–藻类[M]. 杭州: 浙江大学出版社, 2002: 33-143.
[22] 刘思俭, 李伟新. 江蓠及其栽培[J]. 海洋科学, 1979, 3(1): 16-26.
[23] 刘思俭, 林本松, 曾淑芳, 等. 植物生长刺激素、微量元素、营养盐对细基江蓠孢子萌发影响的试验[J]. 水产学报, 1984, 8(2): 179-184.
[24] 周银环, 唐月娣, 淮永丽, 等. 汞离子对细基江蓠孢子萌发和生长的影响[J]. 湛江海洋大学学报, 2006, 26(3): 36-40.
[25] BELLAS J. Comparative toxicity of alternative antifouling biocides on embryos and larvae of marine invertebrates[J]. Sci Total Environ, 2006, 367(2/3): 573-585.
[26] CHO J Y, KWON E, CHOI J, et al. Antifouling activity of seaweed extracts on the green alga Enteromorpha prolifera and the mussel Mytilus edulis[J]. J Appl Phycol, 2001, 13(2): 117-125.
[27] DA GAMA B A P, PEREITA R C, SOARES A R, et al. Is the mussel test a good indicator of antifouling activity? A comparison between laboratory and field assays[J]. Biofouling, 2003, 19(Suppl): 161-169.
[28] SERA Y, ADACHI K, FUJII K, et al. Isolation of haliclonamides: new peptides as antifouling substances from a marine sponge species, Haliclona[J]. Mar Biotechnol, 2002, 3(5): 441-446.

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