收稿日期: 2010-09-30
修回日期: 2010-11-30
网络出版日期: 2011-07-20
基金资助
国家高技术研究发展计划重点项目(2009AA064401); 珠海市科技局项目(PC20081008 )
Effect of selenium on antioxidant enzyme activity and malondialdehyde content of Porphyridium sp.
Received date: 2010-09-30
Revised date: 2010-11-30
Online published: 2011-07-20
利用添加 0.5mg·L−1硒的人工海水(ASW)培养基培养紫球藻 Porphyridium sp. UTEX 637, 并采用试剂盒、氯
化硝基四氮唑蓝(NBT)光化学还原反应法、紫外分光光度法、硫代巴比妥酸(TBA)分析法测定谷胱甘肽过氧化物酶
(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性及丙二醛(MDA)的含量, 着重探讨硒对紫球藻抗氧化
酶活性及丙二醛含量的影响。结果表明, 硒明显促进紫球藻细胞的 GSH-Px 活性(p<0.05)。加硒处理组和未加硒对
照组紫球藻的 GSH-Px 活性均在第 15 天达到最高; 加硒组紫球藻的最大酶活性达到 4.31 个酶活力单位(U), 为对
照组的 1.31 倍。SOD 和 CAT 的活性分别在第 18 天和 15 天达到最大值, 但加硒组与对照组之间差异不显著
(p>0.05), 说明 0.5mg·L−1硒对紫球藻的 SOD 和 CAT 活性影响不明显。加硒能明显降低藻细胞丙二醛的含量, 加
硒组藻细胞的丙二醛含量比对照组藻细胞的丙二醛含量降低了 15.21%。
王江宏,刘艳,桑敏,李爱芬,张成武 . 硒对紫球藻抗氧化酶活性及丙二醛含量的影响[J]. 热带海洋学报, 2011 , 30(3) : 94 -98 . DOI: 10.11978/j.issn.1009-5470.2011.03.094
The effect of selenium on Porphyridium sp. UTEX 637 major antioxidant enzyme system, including glutathione
peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) activities, and malondialdehyde (MDA) content, were
investigated. Porphyridium sp. was cultured in artificial seawater ASW medium containing 0.5mg·L−1
selenium. GSH-Px,
SOD, CAT activities, and MDA content were determined by kits, nitroblue tetrazolium (NBT) photoreduction, UV spectro-
photometry, and thiobarbituric acid (TBA) analysis, respectively. The results show that selenium improved the antioxidation
activity of Porphyridium sp. by increasing the activity of GSH-Px (p<0.05). The GSH-Px activity of Se-treated group and
control group reached the highest on the 15th day, and the GSH-Px activity of Se-treated group was heightened to 4.31U,
which was 1.31 fold higher than that of the control group. SOD and CAT activities reached the maximum on the 18th day and
15th day, respectively, but the differences between the experimental group and the control were not significant (p>0.05). The
activity of SOD and CAT was not significant at the 0.5mg·L−1
concentration of selenium. The content of MDA in Se-treated
group was 15.21% lower than that in the control group.
Key words： Porphyridium sp.; selenium; antioxidant enzyme; malonaldehyde
[1] 郑文杰, 欧阳政. 植物有机硒的化学及其医学应用[M]. 广州: 暨南大学出版社, 2001: 7-9.
[2] MARGARET P R. The importance of selenium to human health[J]. The Lancet, 2000, 356: 233-241.
[3] 黄峙, 郑文杰, 向军俭, 等. 含硒藻蓝蛋白抗小鼠实验性肝损伤的作用[J]. 中国病理生理杂志, 2002, 18(7): 819-822.
[4] 张跃平, 王大志, 高亚辉, 等. 硒在极大螺旋藻细胞中的分布特点及对生化组成的影响[J]. 海洋科学, 2007, 31(2): 31-34
[5] 郑文杰, 贺鸿志, 黄峙, 等. 螺旋藻富集和转化硒研究进展[J]. 中国生物工程杂志, 2003, 23(1): 57-60.
[6] 王大志, 高亚辉, 程兆第. 硒对两种盐藻生长的影响及其在细胞中的累积和分布[J]. 海洋学报, 1997, 19(5): 110-115.
[7] 吕蓉, 张波. 硒浓度对小球藻生长、生物富集的影响[J]. 盐业与化工, 2007, 37(4): 53-55.
[8] 陈必链, 林小建, 施巧琴, 等. 绿色巴夫藻富集锌和硒的研究[J]. 药物生物技术, 1998, 5(1): 25-29.
[9] 刘艳, 桑敏, 李爱芬, 等. 硒在紫球藻蛋白质、多糖和脂类物质中的累积与分布[J]. 中国海洋药物, 2010, 29(3): 24-27.
[10] 朱劲华, 王敏, 张威, 等. 硒化紫球藻胞外多糖毒性及抗氧化活性的研究[J]. 中国海洋药物, 2008, 27(4): 48-51.
[11] 朱劲华, 王敏, 张威, 等. 硒化紫球藻胞外多糖体外诱导BEL-7402肿瘤细胞凋亡的实验研究[J]. 中国海洋药物, 2007, 26(6): 36-39.
[12] 黄峙, 郑文杰, 郭宝江. 含硒生物大分子化合物研究进展[J]. 海南大学学报: 自然科学版, 2001, 19(2): 169-175.
[13] 陈思嘉, 杨芳, 郑文杰. 硒剂量对钝顶螺旋藻的生理生化影响[J]. 海洋学报, 2007, 29(6): 87-92.
[14] 陈思嘉, 杨芳, 郑文杰, 等. 硒胁迫对钝顶螺旋藻抗氧化酶系统的影响[J]. 水生生物学报, 2007, 31(5): 706-711.
[15] 李亚男, 宋德宝, 陈大清. 硒对盐藻生长及抗氧化酶活性的影响[J].长江大学学报:自然科学版, 2006, 3(4):198-210.
[16] 曹吉祥. 用途广泛的紫球藻[J]. 中国海洋药物, 1994, 13(3): 55-56.
[17] 王敏, 朱劲华, 马宇翔, 等. 含硒紫球藻胞外多糖的制备及对体内外肿瘤细胞生长的影响[J]. 海洋科学, 2006, 30(1): 23-27.
[18] 刘太胜, 李爱芬, 顾继光, 等. 硒对紫球藻生长及光谱特性的影响[J]. 生态环境, 2006, 15(2): 224-228.
[19] JONES R F, SPEER H L, KURY W. Studies on the growth of the red alga Porphyridium cruentum[J]. Physiol Plant, 1963, 16(3): 636-643.
[20] BEWLEY T D. Physiological aspects of desiccation tolerance[J]. Ann Rev Plant Physiol, 1979, 30: 195-238.
[21] UPADHYAYA A, SANKHLA D, DAVIS T D, et al. Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in sensing soybean leaves[J]. J Plant Physiol, 1985, 121(5): 453-461.
[22] HERNANDEZ J A, ALMANSA M S. Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves[J]. Physiol Plant, 2002, 115(2): 251-257.
[23] 薛泰麟, 侯少范, 谭见安, 等. 硒在高等植物体内的抗氧化作用Ⅰ. 硒对过氧化作用的抑制效应及酶促机制的探讨[J]. 科学通报, 1993, 38(3): 274-277.
[24] 吴秀峰, 陈平. 硒对水稻幼苗生长和生理特性的影响[J]. 农业与技术, 2004, 24(5): 76-79.
[25] YOKOTA A, SHIGEOKA S, ONISHI T, et al. Selenium as inducer of glutathione peroxidase in low-CO2-grown Chlamydomonas reinhardtiil[J]. Plant Physiol, 1988, 86(3): 649-651.
[26] SHIGEOKA S, TAKEDA T, HANAOKA T, et al. Characterization and immunological properties of selenium-containing glutathione peroxidase induced by selenite in Chlamydomcas reinhandtii[J]. Biochem J, 1991, 275(3): 623-627.
[27] 王艳, 熊德甫, 陈杏, 等. 无机盐对锥状斯氏藻抗氧化酶活性的影响[J]. 华南师范大学学报: 自然科学版, 2009(2): 90-95.
/
| 〈 |
|
〉 |
