收稿日期: 2010-06-29
修回日期: 2011-03-28
网络出版日期: 2011-07-20
基金资助
国家高技术研究发展计划项目(2007AA09Z115)
Establishment of in vitro biofilm model and characteristics of biofilm formation of Vibrio harveyi
Received date: 2010-06-29
Revised date: 2011-03-28
Online published: 2011-07-20
采用改良微孔板法建立哈维氏弧菌 Vibrio harveyi TS-628 菌株生物被膜体外模型, 并进一步研究环境因子
对哈维氏弧菌体外形成生物被膜的影响。结果发现, 哈维氏弧菌 TS-628 菌株可以在聚苯乙烯板中形成生物被膜,
并且该菌生物被膜的形成受起始菌液浓度、温度、pH 等理化因子的显著影响。温度为 30℃、NaCl 质量分数为
3%—6%、pH 为 7、孵育时间为 36h 是哈维氏弧菌形成生物被膜的最佳条件。此外哈维氏弧菌在分别经表皮黏液、
鳃黏液、肠道黏液、肝脏提取液、脾脏提取液、肌肉提取液包被的基质上成膜效果显著, 其中在经肝脏提取液包
被的基质上形成的生物被膜量显著高于在其他生物基质上形成的生物被膜量(p<0.01)。
李海平 ,鄢庆枇 ,徐晓津 ,苏永全 ,覃映雪 . 哈维氏弧菌生物被膜体外模型的建立及成膜特性研究[J]. 热带海洋学报, 2011 , 30(3) : 99 -104 . DOI: 10.11978/j.issn.1009-5470.2011.03.099
Modi?ed microtiter-plate test was used to establish an in vitro biofilm model of Vibrio harveyi TS-628 strain. Effect
of environmental factors on bacterial biofilm formation in vitro was also studied. The results show that V. harveyi TS-628
strains could form biofilm in polystyrene plates, and biofilm formation of this bacterium was significantly affected by different
environmental factors, such as temperature, initial bacterial concentration, pH, NaCl concentration, and so on. The optimum
conditions for biofilm formation of V. h ar v e y i TS-628 strain are at temperature of 30℃, pH=7, NaCl concentration of
3%−6%, and using 36h incubation time. Furthermore, V. harveyi TS-628 strain could form biofilm on the walls coated with
skin mucus, gill mucus, intestinal mucus, liver extract, spleen extract, and muscle extract. The biofilm formation of V. ha r v e y i
on the walls coated with liver extract exhibited a remarkably higher value of D(590)(p<0.01)than that on the walls coated with
other tissue extracts.
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