应用聚合酶链式反应 Polymerase Chan Reacton, PCR 和 环介导等温扩增 Loop-Medated Isothermal Amplcaton, LAMP 两种检测技术分别对 2009 年广东省粤西地区养殖体系中凡纳滨对虾 Ltopenaeus vanname 白斑综合症病毒 Whte Spot Syndrome, WSSV 、桃拉病毒 Taura Syndrome Vrus, TSV 和传染性皮下及造血组织坏死病毒 Infectous Hypodermal and Hematopoetc Necross Vrus, IHHNV 的携带情况进行了调查。结果显示， WSSV 和 TSV 的携带率较高 , 是该地区凡纳滨对虾的主要流行病毒种类 ; LAMP 检测 方法与 PCR 方法具有相当的灵敏度和特异性 , 但 LAMP 检测方法更加简单方便、快速且成本低 。
In order to investigate the infected rates of White Spot Syndrome Virus (WSSV), Taura Syndrome Virus (TSV) and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) among aquatic Litopenaeus vannamei, we tested the samples from western Guangdong Province using Polymerase Chain Reaction (PCR) and Loop-mediated Isothermal Amplication (LAMP). The results show that there were higher infected rates of WSSV and TSV, which suggests these two viruses were the dominant pathogens in this region. These data reveal that the LAMP method was equivalent to the PCR method in specificity and sensitivity, but the LAMP method was more convenient, rapid and low cost than the PCR.
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