收稿日期: 2011-02-19
修回日期: 2011-04-17
网络出版日期: 2012-10-24
基金资助
中国科学院重要方向项目(KSCX2-YW-R-093); 海洋公益性行业科研专项 (200705026)
Effects of nitrate and phosphate on the growth and toxin production of Amphidinium klebsii (Sanya strain)
Received date: 2011-02-19
Revised date: 2011-04-17
Online published: 2012-10-24
以改良K培养液为基础, 通过设定不同的N、P浓度水平, 探讨N、P营养盐对克氏前沟藻Amphidinium klebsii(三亚株)生长及产毒特性的影响。研究结果表明, N、P均为影响克氏前沟藻生长和产毒的重要因素。低浓度的N、P(N浓度≤4.665×10-4mol•L-1, P浓度≤2.000×10-5mol•L-1)不利于克氏前沟藻生物量的积累, 但能促进其生长及毒素的产生; 中浓度的N、P(4.665×10-4mol•L-1-1, 2.000×10-5mol•L-1-5 mol•L-1)有利于克氏前沟藻生物量的积累, 却不利于毒素的产生; N、P浓度过高(N浓度≥37.320×10-4mol•L-1, P浓度≥16.000×10-5mol•L-1)则会抑制克氏前沟藻的生长, 但能促进毒素的产生, 并有相对较高的细胞终密度。克氏前沟藻生长的最佳N、P浓度范围分别为4.665×10-4—37.320×10-4mol•L-1和2.000×10-5 —16.000×10-5mol•L-1。当N浓度为9.330×10?4mol•L-1, P浓度为4.000×10-5mol•L-1时, 克氏前沟藻达到最高密度1.237×105mL-1, 单位体积活血活性达到最高值347.48HU•L-1。
龙超 , 龙丽娟 . 氮、磷对克氏前沟藻三亚株生长及产毒特性的影响[J]. 热带海洋学报, 2012 , 31(4) : 117 -123 . DOI: 10.11978/j.issn.1009-5470.2012.04.016
In order to evaluate the growth and toxin production of Amphidinium klebsii (Sanya strain) under different nitrate and phosphate concentrations, a series of nitrate and phosphate solutions were prepared based on modified K medium. Nitrate and phosphate had significant effects on the growth and toxin productivity of A. klebsii. Low-nitrate and low-phosphate concentrations (N concentration≤4.665×10-4mol•L-1, P concentration≤2.000×10-5mol•L-1) in culture media were not favorable to the biomass accumulation of A. klebsii but favorable to its growth and toxin production. On the contrary, middle-nitrate and middle-phosphate concentrations (4.665×10-4mol•L-1
Key words: Amphidinium klebsii; haemolytic toxin; nitrate; phosphate
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