检索
E-mail
RSS

作者投稿 主编审稿 专家审稿 远程编辑

热带海洋学报 >

2012 , Vol. 31 >Issue 5: 124 - 130

DOI: https://doi.org/10.11978/j.issn.1009-5470.2012.05.018

海洋生物学

饥饿胁迫对九孔鲍免疫防御因子的影响*

  • 陈政强 ,
  • 陈昌生 ,
  • 战文斌 ,
  • 林茂 ,
  • 杨红玲
展开
  • 1. 集美大学水产学院, 福建 厦门361021; 2. 福建省高校水产科学技术与食品安全重点实验室, 福建 厦门361021; 3. 中国海洋大学水产学院, 山东 青岛266003
陈政强(1967—), 男, 副教授, 博士, 从事海洋水产研究。

收稿日期: 2011-09-12

  修回日期: 2012-03-03

  网络出版日期: 2013-02-06

基金资助

福建省自然科学基金资助项目(2006J0420)

收起

Effect of starvation stress on immune response of abalone Haliotis diversicolor supertexta and anti-infectious resistance against Vibrio parahaemolyticus

  • CHEN Zheng-qiang ,
  • CHEN Chang-sheng ,
  • ZHAN Wen-bin ,
  • LIN Mao ,
  • YANG Hong-ling
Expand
  • 1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Key Laboratory of Science and Technology for Aquaculture and Food Safety (Jimei University), Fujian province, Xiamen 361021, China; 3. Fisheries College of Ocean University of China, Tsingtao 266003, China

Received date: 2011-09-12

  Revised date: 2012-03-03

  Online published: 2013-02-06

Fold

摘要

平均体重为14.25±2.21g的九孔鲍在水温24.3°C±1.7°C、盐度为(30.42±1.63)‰、pH值为8.15±0.11的环境条件下, 饥饿胁迫20d是九孔鲍生理上可以承受的。九孔鲍在停食后5d时间内, 血淋巴液蛋白含量没有显著变化，而7d后, 九孔鲍血淋巴液蛋白质含量逐渐大幅度下降, 由此推测, 九孔鲍在饥饿7d后体蛋白被大量消耗于机体的生命维持。饥饿胁迫状态下九孔鲍血淋巴细胞吞噬活性下降, 血淋巴细胞呼吸爆发水平下降, 体液溶菌酶活性、凝集活性下降, 抗感染免疫机能下降; 但是, 九孔鲍经历5d时间的饥饿, 血淋巴抗菌清除效率、血淋巴细胞吞噬活性、血淋巴细胞呼吸爆发产生的超氧阴离子水平、体液溶菌酶活性等免疫指标都变化不大, 说明九孔鲍具有面对饥饿胁迫的应激适应机制。饥饿之初, 机体通过维持既有免疫机能来适应环境、保护自我; 饥饿胁迫持续时间超过7d, 这种本能的逆境适应方式和机能维持终因营养不济和体内资源的持续消耗而改变和衰退。饥饿胁迫下的九孔鲍血淋巴液中的溶菌酶活性和凝集活性下降可能直接由体液蛋白含量下降引起, 而胞内颗粒性物质减少则可能是血淋巴液中溶菌酶及凝集素活性减弱的直接原因。

关键词： 九孔鲍; 饥饿胁迫; 免疫应答; 抗感染力; 副溶血弧菌

本文引用格式

陈政强 , 陈昌生 , 战文斌 , 林茂 , 杨红玲 . 饥饿胁迫对九孔鲍免疫防御因子的影响*[J]. 热带海洋学报, 2012 , 31(5) : 124 -130 . DOI: 10.11978/j.issn.1009-5470.2012.05.018

Abstract

The abalones of H. diversicolor supertexta with an average body weight of 14.25?2.21g can survive over a starvation period of 20 days at 24.3±1.7°C water temperature, (30.42?1.63)‰ salinity, and pH of 8.15?0.11. The concentration of haemolymph protein in the abalones varied slightly in five days when exposed to starvation and substantially dropped progressively after seven days of starvation. Based on these resutls, it is surmised that there was mass humoral protein consumed for life support from 7d to 20d during starvation. The abalones survived with following decreases: a) phagocytosis activity of haemocytes; b) respiratory burst; c) haemolymph lysozyme activity; d) haemolymph agglutination titers and e) anti-infectious immunity during starvation; however, some immune parameters, such as haemolymph clearance efficiency , phagocytosis activity of haemocytes , respiratory burst and haemolymph lysozyme activity, sometimes went up somewhat in the first five days of starvation. Consequently, it is suggested that the abalones are born with functions of starvation stress adaptation. In early period of starvation the abalones did protect themselves and adaptate to surroundings by the way of improving immune effection owing to subnutrition, and this biophilia styles of stress adaptation and natural ability maintaining changed or degenerated from day seven of starvation resulted from seriously poor nutritional state?and excessively?intravital resources degradation. Perhaps the haemolymph lysozyme activity and agglutinating titers decreased resulting from reduction of haemolymph protein, while the intracellular granules reduction may directly result in haemolymph lysozyme activity and agglutinating titers dropped.

Key words： Haliotis diversicolor supertexta; starvation stress; immune response; resistance; Vibrio parahaemolyticus

参考文献

[1] 于赫男, 林小涛, 周小壮, 等. 饥饿胁迫下凡纳滨对虾能源物质的消耗[J]. 海洋科学, 2006, 30(12): 43-46.
[2] 王志铮, 施建军, 吕敢堂, 等. 在短期饥饿胁迫下麦瑞加拉鲮鱼(Cirrhina mrigola)幼鱼的生长、肌体组分及其内脏消化酶活力的变化特征[J]. 海洋与湖沼, 2006, 37(3): 218-223.
[3] 闫智恒, 刘存歧, 王军霞, 等. 饥饿对日本沼虾摄食节律的影响[J]. 四川动物, 2008, 27(5): 843-847.
[4] 李志华, 谢松, 王军霞, 等. 饥饿对日本沼虾生长和部分免疫功能的影响[J]. 上海水产大学学报, 2007, 16(1): 16-21.
[5] 潘沙芳, 李太武, 苏秀榕. 泥蚶血细胞耐饥饿及抗菌力特性的研究[J]. 水产科学, 2007, 26(1): 22-25.
[6] 孙红梅, 黄 权, 丛波. 饥饿对黄颡鱼血液中几种免疫相关因子的影响[J]. 大连水产学院学报, 2006, 21(4): 307- 310.
[7] 孙红梅, 黄权, 丛波, 等. 饥饿对黄颡鱼免疫机能的影响[J]. 水利渔业, 2006, 26(3): 80-81.
[8] 黄辨非, 童响波, 罗静波. 饥饿对泥鳅某些血液指标的影响[J]. 淡水渔业, 2006, 36(6): 33-35.
[9] 陈惠群, 杨文鸽. 饥饿对鳗鲡某些血液指标的影响[J]. 水产养殖, 2002, 23(5): 32-33.
[10] 杜震宇, 刘永坚, 田丽霞, 等. 饥饿对于鲈肌肉、肝脏和血清主要生化组成的影响[J]. 动物学报, 2003, 49(4): 458- 465.
[11] 王沛宾, 林学群, 尹秀芬. 饥饿与恢复投喂对红鳍笛鲷血液生化指标的影响[J]. 水产养殖, 2004, 25(5): 31-34.
[12] 张守领, 顾若波, 徐钢春, 等. 饥饿对于花骨鱼肌肉和血清主要生化指标的影响[J]. 上海交通大学学报: 农业科学版, 2006, 24(6): 513-516.
[13] 钱云霞, 陈惠群, 孙江飞. 饥饿对养殖鲈鱼血液生理生化指标的影响[J]. 中国水产科学, 2002, 9(2): 133-137.
[14] 沈文英, 张利红, 郑永萍, 等. 饥饿对银鲫血液组分和卵巢发育的影响[J]. 动物学研究, 2003, 24(6): 441-444.
[15] GILLIS T E, BALLANTYNE J S. The effects of starvation on plasma free amino acid and glucose concentrations in lake sturgeon[J]. Fish Biology, 1996, 49(6): 1306-1316.
[16] 沈文英, 林浩然, 张为民. 饥饿和再投喂对草鱼鱼种生物化学组成的影响[J]. 动物学报, 1999, 45(4): 404-412.
[17] 翁幼竹, 李少菁, 王桂忠. 饥饿对锯缘青蟹幼体生化组成的影响[J]. 厦门大学学报: 自然科学版, 2002, 41(1): 84-88.
[18] JAYASREE S. Biological properties of a natural agglutinin in the hemolymph of Indian white prawn, Penaeus indicus H. Milne Edwards[J]. Aquaculture, 2001, 194: 245-252.
[19] SONG YENLING, YU CHUN, LIEN T W, et al. Haemolymph parameters of Pacific white shrimp (Litopenaeus vannamei) infected with Taura syndrome virus[J]. Fish & Shellfish Immunology, 2003, 14: 317-313.
[20] 林清华. 免疫学实验[M]. 武汉: 武汉大学出版社, 1999: 73-76, 322-325.
[21] CHENG WINTONG, LIU CHUNHUNG, HSU J P, et al. Effect of hypoxia on the immune response of giant freshwater prawn Macrobrachium rosenberguii and its susceptibility to pathogen Enterococcus[J]. Fish & Shellfish Immunology, 2002, 13: 351-365.
[22] M?RCELO M, RICARDO C, JENNY R, et al. Measurement of reactive oxygen intermediate production in haemocytes of the penaeid shrimp, Penaeus vannamei [J]. Aquaculture, 2000, 191: 89-107.
[23] MOULLAC, L G, SOYEZ C, SAULNIER D, et al. Effect of hypoxia stress on the immune response and the resistance to vibriosis of the shrimp Penaeus stylirostris[J]. Fish & Shellfish Immunology, 1998, 8: 621-629.
[24] JES?S O, ALBERTO C, ALEJANDRO R, et al. Oral administration of yeast, Saccharomyces cerevisiae, enhances the celluar innate immune response of gilthead seabream (Sparus aurata L.) [J]. Vet Immuno Immunop, 2002, 85: 41-50.
[25] MIKULSKI C M, BURNETT L E, BURNETT K G. The effect of hypercapnic hypoxia on the survival of shrimp challenged with Vibrio parahaemolyticus[J]. Shellfish Res, 2000, 19: 301-311.
[26] 罗依惠, 邵健忠, 项黎新, 等. 草鱼血清凝集素的研究[J]. 浙江大学学报: 理学版, 1999, 26(2): 69-74.
[27] 薛江楠, 石安静. 淡水育珠蚌血清凝集素的研究[J]. 四川大学学报: 自然科学版, 2000, 37(2): 242-246.
[28] 邵健忠, 项黎新. 草鱼血清中一种新的凝集素免疫因子[J]. 海洋与湖沼, 2001, 32(5): 519-526.
[29] 管华诗 主编. 海水养殖动物的免疫、细胞培养和病害研究[M]. 济南:山东科学技术出版社, 1999, 10: 32-43.
[30] 杜爱芳. 中国对虾血细胞吞噬功能的研究[J]. 中国水产科学, 1997, 4(2): 1-5.
[31] CHENG WINTON, LI CHIUHSIA, CHEN JIANNCHU. Effect of dissolved oxygen on the immune response of Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus[J]. Aquaculture, 2004, 232: 103-115.
[32] SONG YEN LING, HSIEH Y T. Immunostimulation of tiger shrimp (Penaeus monodon) haemocytes for generation of microbicidal substances: analysis of reactive oxygen species [J]. Dev Comp Immunol, 1994, 18: 201-209.
[33] ADAMS A. Response of penaeid shrimp to exposure to Vibrio species[J]. Fish and Shellfish Immunology, 1991, 1: 59-70.
[34] 张桂蓉, 严安生, 高玉芹, 等. 饥饿对异育银鲫几项血液指标的影响[J]. 水利渔业, 2003, 23(1): 9-10.
[35] 陈晓耘. 饥饿对南方鲶幼鱼血液的影响[J]. 西南农业大学学报, 2000, 22(2): 167-169, 176.
程超, 施光美. 饥饿对鲫鱼血液生理生化指标和流变学性质的影响[J]. 水产渔业科学, 2008, 24(1): 516-519.
Options
文章导航

/

〈 〉