海洋生物学

不同硝酸钠浓度对多形拟绿球藻和模式拟绿球藻生长及脂类积累的影响

  • 张敬键 ,
  • 展望 ,
  • 李爱芬 ,
  • 张成武
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  • 暨南大学水生生物研究中心,热带亚热带水生态工程教育部工程研究中心,广东 广州 510632
张敬键(1984—),女,山东省临沂市人,硕士研究生,从事微藻生物技术及应用研究

收稿日期: 2013-04-20

  修回日期: 2013-04-20

  网络出版日期: 2013-04-20

基金资助

国家高技术研究发展计划重点项目(2009AA064401,2013AA065805);广东省产学研重点项目(2010A090200008)

Effects of sodium nitrate concentration on the growth and lipid accumulation of Pseudochlorococcum polymorphum and Pseudochlorococcum typicum

  • ZHANG Jing-jian ,
  • ZHAN Wang ,
  • LI Ai-fen ,
  • ZHANG Cheng-wu
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  • Institute of Hydrobiology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou 510632, China

Received date: 2013-04-20

  Revised date: 2013-04-20

  Online published: 2013-04-20

摘要

以多形拟绿球藻(Pseudochlorococcum polymorphum UTEX 1791)和模式拟绿球藻(Pseudochlorococcum typicum UTEX 1792)为材料,采用BG-11培养基,研究其在0.3、0.5、1.0和1.5g·L-1等4个硝酸钠浓度条件下的生长和总脂含量。结果表明:适宜多形拟绿球藻生长的硝酸钠浓度范围为0.3—1.0g·L-1,其最终生物质干重在3.7g·L-1左右;模式拟绿球藻在1.0g·L-1的硝酸钠浓度条件下生长最好,可获得4.35g·L-1的生物质干重。在培养过程中两株绿藻的叶绿素a含量与总脂的含量关系密切,在培养初期各实验组叶绿素a含量增加,藻体的总脂含量增长不明显,而在培养中期藻细胞叶绿素a的含量增长平缓,总脂含量增长较明显,其中在0.3g·L-1的硝酸钠浓度实验组总脂增长最为显著,多形拟绿球藻增长了11.65%,模式拟绿球藻增长了24.99%。两株绿藻在0.3g·L-1的低氮实验组总脂含量最高,多形拟绿球藻和模式拟绿球藻总脂分别为细胞干重的44.35%和26.28%,在此条件下两株藻的最大总脂收获量分别为0.98g·L-1和1.61g·L-1

本文引用格式

张敬键 , 展望 , 李爱芬 , 张成武 . 不同硝酸钠浓度对多形拟绿球藻和模式拟绿球藻生长及脂类积累的影响[J]. 热带海洋学报, 2013 , 32(1) : 64 -69 . DOI: 10.11978/j.issn.1009-5470.2013.01.009

Abstract

The present work was oriented toward analyzing the growth and total lipid accumulation of two green microalgae, Pseudochlorococcum polymorphumUTEX 1791 and PseudochlorococcumtypicumUTEX 1792, both of which were cultured in the BG-11medium but under different sodium nitrate concentrations of 0.3, 0.5, 1.0, and 1.5 g·L-1. The results showed that the sodium nitrate concentration, ranging from 0.3 to 1.0g·L-1 was suitable for the growth of P. polymorphumUTEX 1791: its final dry biomass weight was about 3.7g·L-1. The optimum concentration of sodium nitrate for P.typicumUTEX 1792 was 1.0 g·L-1, and its final dry biomass weight reached 4.35g·L-1. The close relationship between the variation of chlorophyll a content and the accumulation of total lipid was observed from these two algal cultures. At the early growth phase, the content of chlorophyll ain all experimental groups increased sharply, while the total lipid accumulation was not significant. However, in the mid growth phase, the content of chlorophyll aincreased slowly, but the total lipid content increased significantly in all experimental groups, especially in the 0.3g·L-1 of sodium nitrate group, whose total lipid content increased by 11.65% (UTEX1791) and 24.99% (UTEX 1792), the maximum total lipid content and yield were up to 26.28% (dry weight)(UTEX1791) and 44.35% (dry weight) (UTEX 1792), 0.98 g·L-1 (UTEX 1791) and 1.61 g·L-1 (UTEX 1792), respectively.

参考文献

[1] SHEEHAN J, DUNNAHAY T, BENEMANN J,et al. A look back at the U. S. Department of Energy’s Aquatic Species Program:Biodiesel from Algae[R]. The National Renewable Energy Laboratory of the U. S. Department of Energy, 1998:328.
[2] 梅洪,张成武,殷大聪,等. 利用微藻生产可再生能源研究概况[J]. 武汉植物学研究, 2008,266:650-660.
[3] HU Q,SOMMERFELD M,JARVIS E,et al. Microalgal triacyglycerols as feedstocks for biodiesel production:Perspectives and advances[J]. The Plant Journal, 2008,54:621-639.
[4] 缪晓玲,吴庆余. 微藻生物质可再生能源的开发利用[J]. 可再生能源, 2003,109:14-16.
[5] CHEN F,JOHNS M R. Effect of C/ N ratio and aeration on the fatty acid composition of heterotrophic Chlorella sorokiniana [J]. Journal of Applied Phycology, 1991,3:203-209.
[6] 梁英,麦康森,孙世春,等. 硝酸钠浓度对三角褐指藻Phaeodactylum tricornutum MACC/ B226 生长及脂肪酸组成的影响[J ]. 海洋科学: 2002,265:48-51.
[7] KHOZIN-GOLDBERG I, COHEN Z. The effect of phosphate starvation on the lipid and fatty acid composition of the fresh water eustigmatophyte Monodus subterraneus [J]. Phytochemistry, 2006,67:696-701.
[8] SOLOVCHENKO A E, KHOZIN-GOLDBERG I, DIDI- COHEN S,et al. Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa [J]. Journal of Applied Phycology, 2008,203:245-251.
[9] ZOU NING, RICHMOND A. Effect of light-path length in outdoor flat plate reactors on output rat of cell mass and of EPA in Nannochloropsis sp. [J]. Journal of Biotechnology, 1999,70:351-356.
[10] RENAUD S M, THINH L V, LAMBRINIDIS G,et al. Effect of temperature on growth,chemical composition and fatty acid composition of tropical Australian microalgae grown in batch cultures[J]. Aquaculture, 2002,2111-4:195-214.
[11] RENAUD S M, Zhou H C, Parry D L,et al. Effect of temperature on the growth,total lipid content and fatty acid composition of recently isolated tropical microalgae Isochrysis sp. , Nitzschia closterium , Nitzschia paleacea ,and commercial species Isochrysis sp. clone T.ISO[J]. Journal of Applied Phycology, 1995, 76:595-602.
[12] 邱昌恩,况琪军,刘国祥,等. 不同氮浓度对绿球藻生长及生理特性的影响[J]. 中国环境科学, 2005,254:408-411.
[13] ILLMAN A M, SCRAGG A H, SHALES S W. Increase in Chlorella strains calorific values when grown in low nitrogen medium [J]. Enzyme and microbial technology, 2000,278:631-635.
[14] WIDJAJA A, CHIEN C C, JU Y H. Study of increasing lipid production from fresh water microalgae Chlorella vulgaris[J]. Journal of the Taiwan Institute of Chemical Engineers ,2009, 401: 13-20.
[15] 曹春晖,孙世春,麦康森,等. 氮浓度对四株海洋绿藻总脂含量和脂肪酸组成的影响[J]. 海洋湖沼通报, 2006,3:79-84.
[16] AZOV Y. Effect of pH on Inorganic Carbon Uptake in Algal Cultures[J]. Appl Environ Microbiol, 1982,436:1300-1306.
[17] KHOZIN-GOLDBERG I, SHRESTHA P, COHEN Z. Mobilization of arachidonyl moieties from triacylglycerols into chlorplastic lipids following recovery from nitrogen starvation of microalga Parietochloris incisa [J]. Biochimica et Biophysica Acta, 2005,1738:63-71.
[18] PINCHETTI J L G, FEMANDEZ E D C, DIEZ P M,et al. Nitrogen availability influences the biochemical composition and photosynthesis of tank-cultivated Ulva rigida Chlorophyta [J]. Journal of Applied Phycology, 1998,10:383-389.
[19] 张艳燕,陈必链,刘梅. 不同硝酸钾浓度对蔷薇藻生长及生理特性的影响[J]. 武汉植物学研究, 2008,261:76-80.
[20] YONGMANITCHAI W, WARD O P. Growth and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions[J]. Applied and Environmental Microbiology, 1991,572:419-425.
[21] EVANS R J, FELBECK G T. High temperature simulation of petroleum formation: Ⅲ. Effect or organic starting material structure on hydrocarbon formation[J]. Organic Geochemistry, 1983,43-4:153-160.
[22] RIFFITHS M J, HARRISON S T L. Lipid productivity as a key characteristic for choosing algal species for biodiesel production [J]. Journal of Applied Phycology, 2009,21:493-507.
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