收稿日期: 2011-12-22
修回日期: 2012-02-20
网络出版日期: 2013-08-28
Preliminary identification of 18 marine fungal strains from the South China Sea and screening of their fermentation products for cytotoxic and antibacterial activities
Received date: 2011-12-22
Revised date: 2012-02-20
Online published: 2013-08-28
张玲 , 张庆波 , 陈玉婵 , 李浩华 , 王磊 , 章卫民 . 18株南海海洋真菌的初步鉴定及其发酵产物的细胞毒活性和抗菌活性筛选[J]. 热带海洋学报, 2013 , 32(3) : 47 -51 . DOI: 10.11978/j.issn.1009-5470.2013.03.007
Eighteen strains of marine fungi were isolated from the sediments of the South China Sea and primarily identified by intergenic transcribed spacer (ITS) sequencing. Cytotoxic and antibacterial activities of their fermentation products were evaluated using the 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) method. The results showed that five strains displayed cytotoxic activity against human breast adenocarcinoma cell line (MCF-7) with an inhibition rate of above 90%; fifteen strains exhibited bacterial inhibition against Staphylococcus aureusat the rate above 90%, accounting for 83.3% of the total strains isolated; two strains showed bacterial inhibition against Escherichia coliat the rate above 90% and two strains showed bacterial inhibition against Pseudomonas aeruginosaat the rate above 90%; and one strain was found to show strong bacterial inhibition against the three tested bacteria.
Key words: marine fungi; cytotoxic; antibacterial
/
〈 | 〉 |