海洋生物学

产酯酶海洋中度嗜盐菌Halomonas sp. LYG1-1的分离鉴定及其酶学性质

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  • 国家海洋局第三海洋研究所海洋生物遗传资源重点实验室, 福建 厦门361005
王昭凯(1979~), 男, 山东省潍坊市人, 助理研究员, 硕士, 研究方向海洋微生物学。E-mail: wzkaicn@163.com

收稿日期: 2012-12-19

  修回日期: 2013-03-01

  网络出版日期: 2014-03-08

基金资助

福建省自然科学基金资金项目(2012J01174)

Isolation, identification and characterization of an esterase-producing marine moderately halophilic strain Halomonas sp.LYG1-1

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  • Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

Received date: 2012-12-19

  Revised date: 2013-03-01

  Online published: 2014-03-08

摘要

以从海南三亚近海海水中采集的样品为研究对象, 利用平板筛选法筛到一株产酯酶的中度嗜盐菌菌株LYG1-1。通过形态学、生理生化及16S rRNA 序列分析, 发现该菌株为革兰氏阴性菌, 其最适生长温度为30℃, 最适生长pH 为7.5, 最适NaCl浓度为10%。16S rRNA基因序列分析显示, 菌株LYG1-1与Halomonas salina 亲缘性最近, 16S rRNA基因序列相似性为99.72%。在含10% NaCl的0.05mol·L-1 Tris-HCl(pH 8.0)缓冲液中, 45℃条件下作用, 酶活性最高, 40℃处理24h仍保持83%的活性。金属离子浓度为5mmol·L-1时, Ca2+、Mg2+对菌株LYG1-1酶活具有明显的激活作用, Hg2+表现出强烈的抑制作用。

本文引用格式

王昭凯, 易志伟, 刘洋, 产竹华, 曾润颖 . 产酯酶海洋中度嗜盐菌Halomonas sp. LYG1-1的分离鉴定及其酶学性质[J]. 热带海洋学报, 2014 , 33(1) : 69 -73 . DOI: 10.11978/j.issn.1009-5470.2014.01.009

Abstract

Using plate screening method, a moderately halophilic strain LYG1-1 was isolated from seawater sample offshore of Sanya. According to physiological and biochemical characterizations, 16S rRNA sequences and phylogenetic analysis, cells were Gram-negative. It grew best at 30℃, pH 7.5 and with the presence of 10% NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belonged to the genus Halomonas, having the highest similarity (99.72%) with Halomonas salina. The maximum esterase activity was observed at 45℃, pH 8.0. The effects of various divalent cations were studied based on the activity of esterase from Halomonas sp. The concentrations of 5 mmol·L-1 Mg2+ and Ca2+ enhanced the esterase activity of LYG1-1, while the presence of Hg2+ strongly inhibited its activity.
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