根据2012年和2013年南海南部渔业资源调查采集到的3435尾鸢乌贼样品的胴长(ML)、右第一腕长(AL1)、右第二腕长(AL2)、右第三腕长(AL3)、右触腕长(TL)、鳍长(FL)、鳍宽(FW)等形态指标的测定结果, 运用种群分类、主成分分析和逐步判别函数等方法对其个体的形态学差异进行研究。结果表明: 南海南部鸢乌贼中型群和微型群具有不同的ML分布, 雌性个体中两个种群的ML非重叠范围为59~78mm和126~225mm, 包含45%的中型群和10%的微型群; 雄性个体中两个种群的ML非重叠范围分别为56~80mm和95~151mm, 包含98%的中型群和58%的微型群。均数差异显著性检验表明, 鸢乌贼两个种群的雌、雄个体在各形态比参数上均存在显著差异(p<0.05): 微型群雌性和雄性个体在AL1/ML、FL/ML、FW/ML上存在显著差异(p<0.05); 中型群雌性和雄性个体在AL1/ML、AL2/ML、AL3/ML、TL/ML、FL/ML、FW/ML上存在显著差异(p<0.05)。差异系数(C.D)计算表明, 鸢乌贼两个种群的形态差异总体上处于种内水平(除雄性FW/ML外, C.D均小于1.28), 而雄性鸢乌贼中两个种群的FW/ML差异达到了亚种水平(C.D=1.45)。主成分分析发现第二和第三主成分分别代表各腕之间和腕与鳍之间的生长差异。依据各形态比参数进行判别分析, 鸢乌贼雌性和雄性个体中两个种群的总体判别准确度分别达到85.9%和92.9%, 可以为鸢乌贼种群区分提供参考。
From 3435 specimens of purple flying squid Sthenoteuthis oualaniensis collected in 2012 and 2013 in the southern South China Sea, mantle length (ML) and other seven morphological indicators were measured. The morphological variations among the squid species were assessed using population classification, principal component analysis and stepwise discriminant functions. Results showed that medium and dwarf forms have different ML distribution. The non-overlapping ranges of ML are 59~78 mm and 126~225 mm for medium and dwarf forms in female individuals, respectively, which contain 45% of medium form and 10% of dwarf form. The non-overlapping ranges of ML are 56~80 mm and 95~151 mm for medium and dwarf forms in male individuals, respectively, which contain 98% of medium form and 58% of dwarf form. Significant differences in all the ratios of the seven morphological indicators to ML between the medium and dwarf forms of males and females were found by tests of significance (p<0.05). Significant differences between males and females in AL1 (Arm Length-I)/ML, FL (Fin Length)/ML, and FW (Fin Width)/ML were identified in the dwarf form (p<0.05), while significant differences between males and females in AL1/ML, AL2/ML, AL3/ML, TL (Tentacle Length)/ML, FL/ML, and FW/ML were found in the medium form (p<0.05). The analysis of coefficients of difference (C.D) indicated that the morphological variations between the medium and dwarf forms generally were within species (C.D were less than 1.28 except for FW/ML of males), but the FW/ML variations between the medium and dwarf forms of males attained a level of difference between subspecies (C.D=1.45). Principal component analysis indicated that the second principal component represents the growing differences among the wrists and fins, and the third principal component represents the growing differences among the wrists. The discriminant functions based on the morphological ratios have the identification accuracy of 85.9% and 92.9%, respectively, for the two forms of females and males, which can provide a reference for classification of squid species.
[1] 陈新军, 2004. 渔业资源与渔场学[M]. 北京: 海洋出版社: 27–28. CHEN XINJUN, 2004. Fishery resource biology and fishing[M]. Beijing: China Ocean Press: 27–28 (in Chinese).
[2] 陈新军, 刘必林, 王尧耕, 2009. 世界头足类[M]. 北京: 海洋出版社: 312–313. CHEN XINJUN, LIU BILIN, WANG YAOGENG, 2009. The world Cephalopods[M]. Beijing: China Ocean Press: 312–313 (in Chinese).
[3] 杜荣骞, 2003. 生物统计学[M]. 2版. 北京: 高等教育出版社: 70–81. DU RONGQIAN, 2003. Biostatistics[M]. 2nd ed. Beijing: Higher Education Press: 70–81 (in Chinese).
[4] 范江涛, 邱永松, 陈作志, 等, 2015. 南海鸢乌贼两个群体间角质颚形态差异分析[J]. 中国海洋大学学报(自然科学版), 45(10): 42–49. FAN JIANGTAO, QIU YONGSONG, CHEN ZUOZHI, et al, 2015. Morphological difference of the beak between two stocks of Sthenoteuthis oualaniensis inhabiting South China Sea[J]. Periodical of Ocean University of China, 45(10): 42–49 (in Chinese).
[5] 江艳娥, 张鹏, 林昭进, 等, 2015. 南海外海鸢乌贼耳石形态特征分析[J]. 南方水产科学, 11(5): 27–37. JIANG YAN’E, ZHANG PENG, LIN ZHAOJIN, et al, 2015. Statolith morphology of purpleback flying squid (Sthenoeuthis oualaniensis) in the offshore South China Sea[J]. South China Fisheries Science, 11(5): 27–37 (in Chinese).
[6] 李朋, 2014. 南海鸢乌贼的种群遗传结构[D]. 上海: 上海海洋大学: 1–65. LI PENG, 2014. Population genetic structure of Sthenoteuthis oualaniensis in South China Sea[D]. Shanghai: Shanghai Ocean University: 1–65 (in Chinese).
[7] 李思发, 李晨虹, 李家乐, 1998. 尼罗罗非鱼品系间形态差异分析[J]. 动物学报, 44(4): 450–457. LI SIFA, LI CHENHONG, LI JIALE, 1998. Analysis of morphological variations among strains of Nile tilapia (Oreochromis niloticus)[J]. Acta Zoologica Sinica, 44(4): 450–457 (in Chinese).
[8] 刘必林, 2006. 利用耳石微结构研究印度洋西北海域鸢乌贼的年龄和生长[D]. 上海: 上海水产大学: 12–15. LIU BILIN, 2006. Studying age and growth of purple back flying squid (Sthenoeuthis oualaniensis) in northwest Indian Ocean based on statolith microstructure[D]. Shanghai: Shanghai Ocean University: 12–15 (in Chinese).
[9] 刘金立, 2006. 利用形态学和RAPD方法鉴定印度洋西北部海域鸢乌贼的种群结构[D]. 上海: 上海水产大学: 6–7. LIU JINLI, 2006. Identifying population structure in the northwestern Indian Ocean using morphological and RAPD analysis of the purpleback flying squid Symplectoteuthis oualaniensis[D]. Shanghai: Shanghai Ocean University: 6–7 (in Chinese).
[10] 王尧耕, 陈新军, 2005. 世界大洋性经济柔鱼类资源及其渔业[M]. 北京: 海洋出版社: 190–194. WANG YAOGENG, CHEN XINJUN, 2005. The world ocean economic squid resources and Fisheries[M]. Beijing: China Ocean Press: 190–194 (in Chinese).
[11] 徐克学, 1999. 生物数学[M]. 北京: 海洋出版社: 40–70. XU KEXUE, 1999. Biomathematics[M]. Beijing: China Ocean Press: 40–70 (in Chinese).
[12] 颜云榕, 易木荣, 冯波, 等, 2015. 南海鸢乌贼3个地理群体形态差异与判别分析[J]. 广东海洋大学学报, 35(3): 43–50. YAN YUNRONG, YI MURONG, FENG BO, et al, 2015. Morphological variations and discriminant analysis of three different geographic cohorts of Sthenoteuthis oualaniensis in the South China Sea[J]. Journal of Guangdong Ocean University, 35(3): 43–50 (in Chinese).
[13] 杨德康, 2002. 两种鱿鱼资源和其开发利用[J]. 上海水产大学学报, 11(2): 176–179. YANG DEKANG, 2002. The resources and its exploitation and utilization of two spcies of squid[J]. Journal of Shanghai Fisheries University, 11(2): 176–179 (in Chinese).
[14] 张鹏, 晏磊, 杨炳忠, 等, 2015. 春季南沙海域鸢乌贼种群结构特征的研究[J]. 南方水产科学, 11(5): 11–19. ZHANG PENG, YAN LEI, YANG BINGZHONG, et al, 2015. Population structure of purpleback flying squid (Sthenoteuthis oualaniensis) in Nansha area in spring[J]. South China Fisheries Science, 11(5): 11–19 (in Chinese).
[15] 张莹, 付东洋, 潘德炉, 等, 2013. 逐步判别分析法在海洋水质评价中的应用[J]. 海洋学报, 35(6): 204–209. ZHANG YING, FU DONGYANG, PAN DELU, et al, 2013. The application of a stepwise discriminant analysis method in a marine water quality evaluation[J]. Acta Oceanologica Sinica, 35(6): 204–209 (in Chinese).
[16] ALLMON W D, 1992. A causal analysis of stages in allopatric speciation[J]. Oxford Surveys in Evolutionary Biology, 8: 219–257.
[17] ANNOTATED A N, DATE S K T O, 2005. Cephalopods of the world[J]. FAO Species Catalogue for Fishery Purposes, 1(4): 316–318.
[18] BASIR S, 2000. Biological feature of an oceanic squid, Sthenoteuthis oualaniensis in the South China Sea, area Ⅲ: Western Philippines[C]//THE SECRETARIAT SOUTHEAST ASIAN FISHERIES DEVELOPMENT CENTER. Proceedings of the Third Technical Seminar on Marine Fishery Resources Survey in the South China Sea, area Ⅲ: Western Philippines. Bangkok, Thailand: Southeast Asian Fisheries Development Center: 135–147.
[19] CHEMBIAN A J, MATHEW S, 2014. Population structure of the purpleback squid Sthenoteuthis oualaniensis (Lesson, 1830) along the south-west coast of India[J]. Indian Journal of Fisheries, 61(3): 20–28.
[20] CLARKE M R, 1996. The role of Cephalopods in the world’s oceans: General conclusion and the future[J]. Philosophical Transactions of the Royal Society B: Biological Sciences, 351(1343): 1105–1112.
[21] LABE L L, 2000. Catch rate of oceanic squid by jigging method in the South China Sea, area Ⅲ: Western Philippines[C]//THE SECRETARIAT SOUTHEAST ASIAN FISHERIES DEVELOPMENT CENTER. Proceedings of the Third Technical Seminar on Marine Fishery Resources Survey in the South China Sea, area Ⅲ: Western Philippines. Bangkok, Thailand: Southeast Asian Fisheries Development Center: 19–31.
[22] NESIS K, 1977. Population structure of the squid Sthenoteuthis oualaniensis (Lesson, 1830) in the tropical West Pacific[J]. Trudy IO AN SSSR, 107: 15–29.
[23] NESIS K N, 1993. Population structure of oceanic ommastrephids, with particular reference to Sthenoteuthis oualaniensis: a review[M]//OKUTANI T, O’DOR R K, KUBODERA T. Recent advances in cephalopod fishery biology. Tokyo: Tokai University Press: 375–383.
[24] OKUTANI T, Tung I H, 1978. Reviews of biology of commercially important squids in Japanese and adjacent waters. 1. Symplectoteuthis-oualaniensis (lesson)[J]. Veliger, 21(1): 87–94.
[25] SMITH P J, ROBERTS P E, HURST R J, 1981. Evidence for two species of arrow squid in the New Zealand fishery[J]. New Zealand Journal of Marine & Freshwater Research, 15(3): 247–253.
[26] STAAF D J, RUIZ-COOLEY R I, ELLIGER C, et al, 2010. Ommastrephid squids Sthenoteuthis oualaniensis and Dosidicus gigas in the eastern Pacific show convergent biogeographic breaks but contrasting population structures[J]. Marine Ecology Progress Series, 418(6): 165–178.
[27] SUZUKI T, YAMAMOTO S, ISHII K, et al, 1986. On the flying squid Stenotuthis oualaniensis (Lesson) in Hawaiian waters[J]. Bulletin of the Faculty of Fisheries, 37(2): 111–123.
[28] YOUNG R E, HIROTA J, 1998. Review of the ecology of Sthenoteuthis oualaniensis near the Hawaiian Archipelago[C]// OKUTANI T. International symposium on large pelagic squids. Tokyo: Japan Marine Fishery Resources Research Center: 113–143.