大亚湾核电站邻近水域桡足幼体现场摄食研究
作者简介:徐翠莲(1989—), 女, 湖北省广水市人,博士研究生,从事浮游动物摄食生态学研究。E-mail: xucuilian12b@mails.ucas.ac.cn
收稿日期: 2017-05-21
要求修回日期: 2017-08-14
网络出版日期: 2018-04-11
基金资助
国家重点研发计划课题(2016YFC0502805)
广东省科技计划项目(2015A020216013)
中国科学院战略性先导科技专项(XDA13020100)
国家重点基础研究发展计划(“973”项目) (2015CB452904)
国家自然科学基金项目(41276160)
广东省应用海洋生物学重点实验室运行经费(2017B030314052)
In situ feeding of copepodites in the water near Daya Bay nuclear power plant
Received date: 2017-05-21
Request revised date: 2017-08-14
Online published: 2018-04-11
Supported by
National Key Research and Development Project (2016YFC0502805)
Science and Technology Planning Projects of Guangdong Province, China (2015A020216013)
Strategic Priority Research Program of Chinese Academy of Sciences (XDA13020100)
National Basic Research Program of China (“973” Program) (2015CB452904)
National Natural Science Foundation of China (41276160)
Science and Technology Planning Project of Guangdong Province, China (2017B030314052)
Copyright
桡足类种类多、数量大、分布广, 在食物链中处于中间环节, 对海洋生态系统的结构稳定起着重要作用。桡足类幼体获取的营养直接影响其发育, 进而影响桡足类成体补充乃至种群的稳定。本研究于2015年夏季分别在大亚湾核电站邻近海域S1 (进水口水域)和S2 (排水口水域)站进行了调查采样, 应用分子生物学方法检测了桡足幼体现场摄食食物组成。结果显示: 1)两个站的桡足幼体内共检测到16种不同食物, 包括浮游植物(硅藻)11种, 后生动物2种、真菌、Ichthyosporea 和卵菌类各1种共5大类, 其中硅藻(47.30%, 克隆数百分比, 下同)和被囊动物类后生动物(41.89%)是其主要的食物类群; 2)在S1和S2站桡足幼体内分别检测到9种和10种食物, 但主要食物类型有差异, S1站较多后生动物(61.54%), 而S2站较多硅藻(68.57%); 3) S2站的桡足幼体杂食偏植食程度更高, 其杂食性系数(0.31)低于S1站(0.72)。结果表明, 桡足幼体能根据食物环境有选择地摄食植物饵料和动物饵料, 调节食物营养结构; 温排水影响水域桡足幼体更偏向植食性, 尤其是硅藻, 暗示全球变暖可能导致桡足幼体食性偏移。
徐翠莲 , 李涛 , 胡思敏 , 王有军 , 黄晖 , 刘胜 . 大亚湾核电站邻近水域桡足幼体现场摄食研究[J]. 热带海洋学报, 2018 , 37(2) : 17 -25 . DOI: 10.11978/2017058
Copepods play important roles in maintaining the structure of marine ecosystem due to their key position in food chain and their numerous species, quantities and wide distribution. The nutrients obtained by copepod larvae can directly affect their development, then the complement of adult copepods and even their population stability. In this study, in situ feeding of copepodites was evaluated by molecular methods in the waters near the nuclear power plant in Daya Bay (S1: control station, S2: outfall station) in summer 2015. The results showed that 16 prey species in total were identified in both stations, including diatoms (11 species), tunicate (two species), fungi (one species), ichthyosporea (one species), and oomycetes (one species). Diatoms (47.30%) and tunicate (41.89%) were the most abundant food types revealed by the significant proportion of the clones. Similar feeding diversity was discovered in copepodites at S1 and S2, with nine prey species at S1 and 10 at S2, but significant differences in diet composition were revealed, with metazoan (61.54%) and diatoms (68.57%) dominating the diets of copepodites at S1 and S2, respectively. In addition, copepodites appeared to be more herbivorous at S2 with a lower Omnivory Index (0.31) than at S1 (0.72). These results suggested that copepodites could optimize their diet composition by consuming a reasonable percentage of animal and plant materials according to available food sources. More phytoplankton, especially diatoms as diet of copepodites at the outfall station implied that copepodites might change their feeding habits with global warming development.
Key words: Daya Bay; copepodites; feeding; diversity; increased temperature
Fig. 1 Sampling sites in Daya Bay. Triangle is the location of Daya Bay Nuclear Power Plant and Ling-Ao Nuclear Power Plant. S1: control station; S2: outfall station图1 大亚湾调查和采样站位 |
Tab. 1 Diversity indices and biomass of phytoplankton表1 浮游植物多样性指数及生物量 |
种类数 | 丰度百分比 | 细胞丰度/ (×105cells·L-1) | 香农-威纳指数a | 优势种 | 叶绿素a含量/(μg·L-1) | |||
---|---|---|---|---|---|---|---|---|
甲藻 | 硅藻 | 甲藻 | 硅藻 | |||||
S1 | 7 | 14 | 66.14% | 38.36% | 4.38 | 1.89 | Scrippsiella trochoidea | 5.09 |
S2 | 5 | 12 | 71.94% | 28.06% | 3.92 | 1.39 | 3.18 |
注: a香农-威纳(Shannon-Weaver)指数\(H |
Tab. 2 Diet diversity indices of copepodites表2 桡足幼体现场食物多样性指数 |
样品编号 | 种类数 | 有效克隆数 | 辛普森指数b | 香农-威纳指数c | Chao1 指数d |
---|---|---|---|---|---|
S1 | 9 | 39 | 0.6772 | 1.593 | 10.5 |
S2 | 10 | 35 | 0.8245 | 1.987 | 11 |
注: b辛普森(Simpson)指数\(D=1-\sum^s_{i=1}P^2_i\); Pi=Ni/N, 式中 S是得到的种类数量, Ni 是含有 i 条序列的序列数目, N是总序列的数量(Simpson, 1949)。c香农-威纳(Shannon-Weaver)指数的计算公式同 |
Fig. 2 Relationship between clones and taxon of copepodite diet (a) and neighbor joining tree of 18S rDNA sequences obtained from copepodite diet (b)图2 送测有效克隆样品数所覆盖的种类数(a)和桡足幼体克隆文库测的18S rDNA序列构建的邻接树(b) |
Fig. 3 Prey composition of copepodites. (a) group of prey composition; (b) species of prey composition图3 桡足幼体肠道食物组成 |
The authors have declared that no competing interests exist.
[1] |
|
[2] |
|
[3] |
|
[4] |
|
[5] |
|
[6] |
|
[7] |
|
[8] |
|
[9] |
|
[10] |
|
[11] |
|
[12] |
|
[13] |
|
[14] |
中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会, 2008. GB/T 12763.6-2007 海洋调查规范第6部分: 海洋生物调查[S]. 北京: 中国标准出版社.
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China, 2008. GB/T 12763.6-2007 Specifications for oceanographic survey—Part 6: Marine biological survey[S]. Beijing: China Standard Publishing House (in Chinese).
|
[15] |
|
[16] |
|
[17] |
|
[18] |
|
[19] |
|
[20] |
|
[21] |
|
[22] |
|
[23] |
|
[24] |
|
[25] |
|
[26] |
|
[27] |
|
[28] |
|
[29] |
|
[30] |
|
[31] |
|
[32] |
|
[33] |
|
[34] |
|
[35] |
|
[36] |
|
[37] |
|
[38] |
|
[39] |
|
[40] |
|
[41] |
VAN DE WAAL D B,
|
[42] |
|
[43] |
|
[44] |
|
[45] |
|
/
〈 |
|
〉 |