热带海洋学报

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鲸鱼骨来源真菌Penicillium sp. S2014503的生物活性成分

张海波1, 2, 3, Imran Khan1, 2, Kumar Saurav4, 张丽萍1, 3, 方壮杰1, 2, 张鑫雅1, 2, 彭方5, 张长生1, 2, 3   

  1. 1. 热带海洋生物资源与生态重点实验室,广东省海洋药物重点实验室,中国科学院南海海洋研究所海洋微生物研究中心,广东 广州 510301

    2. 中国科学院大学,北京 100049

    3. 南方海洋科学与工程广东省实验室(广州),广东 广州 511458

    4. 海藻生物技术实验室-海藻技术中心,捷克科学院微生物研究所,捷克共和国 37981

    5. 武汉大学,中国典型培养物保藏中心,湖北 武汉 430072

  • 收稿日期:2022-04-11 修回日期:2022-05-10 出版日期:2022-05-16 发布日期:2022-05-16
  • 通讯作者: 张海波
  • 基金资助:
    南方海洋科学与工程广东省实验室(广州)重大专项团队项目(GML2019ZD0406),国家自然科学基金项目(81872778, 41911530078),国家重点研发计划项目(2018YFC1406705,2018YFC1406704)

Bioactive natural compounds from a whale bone-derived fungus Penicillium sp. S2014503

ZHANG Haibo1,2,3, Imran Khan1,2, Kumar Saurav4, ZHANG Liping1,3, FANG Zhuangjie1,2, ZHANG Xinya1, 2, PENG Fang5, ZHANG Changsheng1,2,3   

  1. 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China;

    2. University of Chinese Academy of Sciences, Beijing 100049, China;

    3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), 1119 Haibin Rd., Nansha District, Guangzhou 511458, China;

    4. Laboratory of Algal Biotechnology-Centre-Algatech, Institute of Microbiology of the Czech Academy of Sciences, Trebon 37981, Czech Republic;

    5. Wuhan University, China Center for Type Culture Collection, Wuhan 430072, China

  • Received:2022-04-11 Revised:2022-05-10 Online:2022-05-16 Published:2022-05-16
  • Contact: Haibo Zhang
  • Supported by:
    Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0406); National Natural Science Foundation of China (81872778, 41911530078); National Key R&D Program of China (2018YFC1406705, 2018YFC1406704)

摘要: 从一块腐烂的鲸鱼骨头分离鉴定了一株真菌Penicillium sp. S2014503。利用大米固体发酵培养技术从该菌产物中获得10个化合物,通过核磁共振波谱数据比对分析将这10 个化合物分别鉴定为emodin (1)、citreorosein (2)、tetrahydro altersolanol B (3)、conioxanthone A (4)、chrysogine (5)、pyramidamycin B (6)、germicidin O (7)、 2-(6-hydroxy-5,7-dimethylbenzefuranone-4-yl) acetaldehyde (8)、astrophenone (9)和chenopodolans A (10)。经生物活性测试发现,化合物1和2对藤黄微球菌具有弱的抑制活性,化合物1、2和4对卤虫幼虫具有显著的致死毒性。此外,化合物1是中药大黄和虎杖的主要致泻成分,本研究首次报道了来源于海洋动物样品鲸鱼骨的真菌Penicillium sp.可生产大黄素类等活性天然产物。

关键词: 鲸鱼骨, 青霉菌, 大黄素

Abstract: Penicillium sp. S2014503 was isolated from a rotten whale bone sample. Cultured on a solid state rice medium, the strain S2014503 yielded ten compounds emodin (1), citreorosein (2), tetrahydro altersolanol B (3), conioxanthone A (4), chrysogine (5), pyramidamycin B (6), germicidin O (7), 2-(6-hydroxy-5,7-dimethylbenzefuranone-4-yl) acetaldehyde (8), astrophenone (9), and chenopodolans A (10). Chemical structures of the above mentioned compounds were determined by comparison of the 1H, 13C NMR data of each compound with the reported ones. Bioactivities of all the isolated compounds were evaluated for the antibacterial activities and cytotoxicity against brine-shrimp larvae. Compounds 1 and 2 showed weak activity against Gram positive bacteria Micrococcus luteus. Compounds 1, 2 and 4 were significantly cytotoxic against brine-shrimp larvae (Artemia salina) after 6 hours of incubation at room temperature. Interestingly, compound 1 was a well-known laxative ingredient of Traditional Chinese Medicine Rheum officinale and Polygonam cuspidatum. This work is the first report on the emodin analogs from whale bone derived fungus Penicillium sp. Key words: Whale bone, Penicillium sp., Emodin

Key words: Whale bone, Penicillium sp., Emodin