海洋细菌,假交替单胞菌;基因岛;泳动能力,环境适应性 ," /> 海洋细菌,假交替单胞菌;基因岛;泳动能力,环境适应性 ,"/> marine bacteria,Pseudoalteromonas,genomic island,motility,environmental adaptation ,"/> 海洋假交替单胞菌SM9913中基因岛GIPspSM9913的鉴定和功能研究

热带海洋学报

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海洋假交替单胞菌SM9913中基因岛GIPspSM9913的鉴定和功能研究

王鹏霞1,2,3,4*,赵逸5,杜晓飞1,3,王伟权1,3,王晓雪1,2,3   

  1. 1. 中国科学院热带海洋生物资源与生态重点实验室,中国科学院南海海洋研究所,广州 510301

    2. 南方海洋科学与工程广东省实验室(广州),广州市南沙区南沙街资讯科技园海滨路1119号,广州 511458

    3. 中国科学院大学,北京 100049

    4. 三亚中科海洋研究院,崖州湾深海科技城,三亚 572000

    5. 河北师范大学,生命科学学院,石家庄 050024

  • 收稿日期:2022-05-06 修回日期:2022-06-29 出版日期:2022-07-01 发布日期:2022-07-01
  • 通讯作者: 王鹏霞
  • 基金资助:
    海南省科技计划三亚崖州湾科技城联合项目(320LH047); 广东省自然科学基金(2019A1515011912); 广州市科技计划项目(202002030493)

Identification and functional study of the genomic island GIPspSM9913 in Pseudoalteromonas sp. SM9913

WANG Pengxia1,2,3,4*, ZHAO Yi5, DU Xiaofei1,3, WANG Weiquan1,3, WANG Xiaoxue1,2,3   

  1. 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;

    2. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No.1119, Haibin Road, Nansha District, Guangzhou 511458, China

    3. University of Chinese Academy of Sciences, Beijing 100049, China

    4. Sanya Institute of Oceanology, SCSIO, Yazhou Scientific Bay, Sanya 572000, China

    5. College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei, China

  • Received:2022-05-06 Revised:2022-06-29 Online:2022-07-01 Published:2022-07-01
  • Contact: Pengxia Wang
  • Supported by:

    the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City (320LH047), the Natural Science Foundation of Guangdong Province (2019A1515011912), and the Science and Technology Planning Project of Guangzhou (202002030493).

摘要: 海洋细菌由于所处环境的复杂性和多变性,进化出独特的环境适应机制。基因岛(Genomic islands)通常携带宿主菌环境适应性有关的基因,在推动细菌环境适应性和基因组多样化中起重要作用。假交替单胞菌属(Pseudoalteromonas)在各种海洋生境中广泛分布,具有重要的工业应用价值和生态修复潜力。本研究以深海沉积物中分离的假交替单胞菌SM9913为研究对象,通过与表层海水中分离的假交替单胞菌TAC125进行比较基因组学分析,发现SM9913基因组上yicC基因内部整合了一个18 kb的基因岛(我们命名为GIPspSM9913)。该基因岛编码整合酶、切离酶以及多套限制修饰系统。数据库检索分析发现GIPspSM9913同源的基因岛在假交替单胞菌、希瓦氏菌(Shewanella)弧菌(Vibrio)和气单胞菌属(Aeromonas)等细菌中广泛存在。定量PCR结果显示切离酶表达时,GIPspSM9913会从基因组上大量切离,产生基因岛发生切除的SM9913突变株。测序分析表明GIPspSM9913的切除不会影响整合位点侧翼基因的表达。比较切离突变株与野生菌的运动能力、电转化频率和接合转移效率等,发现GIPspSM9913可以提高宿主细菌的泳动能力和对外源DNA入侵的抵御能力。

关键词: 海洋细菌')">

海洋细菌, 假交替单胞菌;基因岛;泳动能力, 环境适应性

Abstract: Due to the complexity and variability of marine environments, marine bacteria may have evolved unique environmental adaptation mechanisms. Genomic islands usually carry genes related to the environmental adaptation of host bacteria and play an important role in driving bacterial adaptation and genome diversification. Pseudoalteromonas is an important genus that is widely distributed in various marine habitats, and has attracted attention due to the important industrial application and ecological restoration potential. In this study, we focused on Pseudoalteromonas sp. SM9913 isolated from marine sediments. By comparative genomics analysis of SM9913 with the closely related strain Pseudoalteromonas haloplanktis TAC125 isolated from surface seawater, an 18-kb genomic island GIPspSM9913 integrated in the yicC gene was identified. This genomic island encodes an integrase and an excisionase, as well as multiple restriction modification systems. Sequence analysis showed that GIPspSM9913 homologs are widely distributed in marine bacteria such as Pseudoalteromonas, Shewanella, Vibrio and Aeromonas. Quantitative PCR assays showed that GIPspSM9913 can be excised when the excisionase is produced, resulting in the removal of GIPspSM9913. Sequencing analysis indicated that excision of GIPspSM9913 did not affect the expression of flanking genes. We also compared the swimming motility, electroporation efficiency and conjugation efficiency of the GIPspSM9913 deleted strain and the wild-type strain SM9913, and found that the presence of GIPspSM9913 can increase the swimming motility of the host bacteria and offer the host defence against the invasion of foreign DNA.

Key words: marine bacteria')">

marine bacteria, Pseudoalteromonas, genomic island, motility, environmental adaptation