热带海洋学报 ›› 2018, Vol. 37 ›› Issue (4): 45-51.doi: 10.11978/2017109CSTR: 32234.14.2017109

• • 上一篇    下一篇

基于线粒体DNA D-loop序列的黄斑篮子鱼群体遗传多样性分析

黄小林1,2(), 李文俊1, 林黑着1,2(), 杨育凯1,2, 李涛1,2, 虞为1,2, 黄忠1,2   

  1. 1. 中国水产科学研究院南海水产研究所, 广东省渔业生态环境重点实验室, 广东 广州 510300
    2. 中国水产科学研究院南海水产研究所深圳试验基地, 广东 深圳 518121
  • 收稿日期:2017-10-12 修回日期:2018-03-31 出版日期:2018-07-20 发布日期:2018-07-16
  • 作者简介:

    作者简介:黄小林(1987—), 男, 助理研究员, 从事水产种质资源与遗传育种研究。E-mail: 499324782@qq.com

  • 基金资助:
    广东省渔港建设与渔业发展专项海洋渔业科技推广方向-科技攻关与研发项目(A201601B10);广东省渔业生态环境重点实验室开放基金资助(FEEL-2017-13);深圳市科技计划知识创新基础研究项目(JCYJ20170817103922921、JCYJ20170817103856495);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2016TS01)

Genetic variations among Siganus oramin populations in coastal waters of southeast China based on mtDNA control region sequences

Xiaolin HUANG1,2(), Wenjun LI1, Heizhao LIN1,2(), Yukai YANG1,2, Tao LI1,2, Wei YU1,2, Zhong HUANG1,2   

  1. 1. Guangdong Provincial Key Laboratory of Fishery Ecology and Environment; South China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510300, China
    2. Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China
  • Received:2017-10-12 Revised:2018-03-31 Online:2018-07-20 Published:2018-07-16
  • Supported by:
    Marine Fishery Science and Technology Industry Projects from Guangdong Province (A201601B10);Guangdong Provincial Key Laboratory of Fishery Ecology and Environment (FEEL-2017-13);Knowledge Innovation Basic Research Project of Shenzhen Science and Technology Program (JCYJ20170817103922921、JCYJ20170817103856495);South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Scientific Research Funds for Central Non-profit Institutes (2016TS01)

摘要:

对中国华南沿海不同地理群体的黄斑篮子鱼(Siganus oramin)进行遗传多样性分析, 测定了漳州东山湾(DS)、深圳大亚湾(DY)、深圳大鹏湾(DP)、阳江沙扒湾(SB) 4个群体共计100尾黄斑篮子鱼线粒体DNA D-loop序列。结果显示: 序列长度约828bp, 包含72个变异位点, 50个单倍型, A、T、C、G含量平均为31.2%、31.1%、20.8%和16.9%; 单倍型多样性指数为0.893±0.053~0.957±0.030, 核苷酸多样性指数为0.0105±0.0031~0.0179±0.0031, 表现为高单倍型多样性而低核苷酸多样性的特点; 群体内的遗传距离0.0107~0.0184, 群体间的遗传距离0.0112~0.0172, AMOVA分析显示群体间的分子变异占0.66%, 群体内占99.34%; 中性检验结果表明Fu’s Fs检验值为显著的负值(-16.7079, p<0.01); 核苷酸不配对分布图呈单峰, 表明黄斑篮子鱼演化过程中经历过种群扩张事件, 推测扩张时间约在1.38~4.60万年前。综上, 华南沿海岸4个黄斑篮子鱼群体间不存在显著的遗传分化, 可划归一个管理单元, 而沙扒湾(SB)群体遗传多样性水平相对较高, 应优先加以保护。

关键词: 线粒体DNA D-loop, 黄斑篮子鱼, 群体遗传多样性

Abstract:

In this study, we determined 828 bp mtDNA control region sequences of 100 Siganus oramin individuals collected in Daya Bay, Dapeng Bay, Shaba Bay, and Dongshan Bay where 72 variable sites defined 50 haplotypes. The parameter of haplotype diversity (h) were 0.893±0.053~0.957±0.030, and that of nucleotide diversity (π) was 0.0105±0.0031~0.0179± 0.0031, indicating a peculiarity of high haplotype diversity and low nucleotide diversity, The genetic diversity indexes of four geographical groups were, respectively, 0.0121 (DS), 0.0169 (DY), 0.0107 (DP), and 0.0184 (SB). A combination of the significant negative neutral test values of Fu’s Fs (-16.7079, p<0.01), the mismatch distribution pattern, and the network analysis revealed that a historical population expansion occurred at about 38300 years ago. The indices of genetic distance among populations (0.0112~0.0172) were at a similar level as the indices of genetic distance within populations (0.0107~0.0184). The overall genetic differentiation index (Fst) was 0.0066 (p>0.05), and the analysis of molecular variance showed that most of the genetic variations of the four populations was distributed within populations. With the above findings, it is suggested that S. oramin in the coastal waters of Southeast China is in low level of population genetic diversity and should receive effective protection.

Key words: mitochondrial D-loop, Siganus oramin, genetic diversity

中图分类号: 

  • P735.541