高温对褐藻羊栖菜逆境生理的影响

doi:10.11978/2020052

热带海洋学报 ›› 2021, Vol. 40 ›› Issue (2): 74-82.doi: 10.11978/2020052CSTR: 32234.14.2020052

高温对褐藻羊栖菜逆境生理的影响

刘丽杰^1,²(), 林立东²()

1.齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006
2.温州市洞头区海洋与渔业发展研究中心, 浙江温州 325700

收稿日期:2020-05-22 修回日期:2020-07-29 出版日期:2021-03-10 发布日期:2020-08-02
通讯作者: 林立东
作者简介:刘丽杰(1980—), 女, 黑龙江省齐齐哈尔市人, 副教授, 博士, 研究方向为植物生理与分子生物学。email: liulijie2001@163.com
基金资助:
温州市洞头区科技计划项目(N2016Y20B);温州市洞头区重大渔农业科技项目(N2018Y03A);黑龙江省教育厅基本业务专项(135209265);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项)(YSTSXK201877)

The effect of high temperature on stress physiology of Sargassum fusiforme

LIU Lijie^1,²(), LIN Lidong²()

1. College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar 161006, China
2. Marine and Fisheries Development Research Center, Dongtou district of Wenzhou City, Wenzhou 325700, China

Received:2020-05-22 Revised:2020-07-29 Online:2021-03-10 Published:2020-08-02
Contact: LIN Lidong
Supported by:
Science and Technology Plan Projects in Dongtou District of Wenzhou City(N2016Y20B);Major Fisheries and Agriculture Science and Technology Projects in Dongtou District of Wenzhou City(N2018Y03A);Special program for basic business of Heilongjiang Provincial Department of Education(135209265);Fundamental Research funds in Heilongjiang Provincial Universities (Special specialty of plant food processing technology)(YSTSXK201877)

摘要/Abstract

摘要：

分别在27℃和32℃高温条件下培养羊栖菜(Sargassum fusiforme), 以在22℃培养条件下生长的羊栖菜作为对照, 在培养的第0、1、3、5和7天分别取气囊样品, 采用植物生理学试验方法探讨高温胁迫对羊栖菜细胞膜损伤、渗透调节和抗氧化保护的影响。结果表明: 1) 高温胁迫导致羊栖菜细胞的相对电导率提高和丙二醛(MDA)质量摩尔浓度增加, 膜系统受到了损伤, 造成体内离子外渗、细胞膜脂发生过氧化; 2) 细胞内积累了可溶性蛋白、可溶性糖和脯氨酸等渗透调节物质并且胁迫组始终高于对照组, 其中可溶性蛋白在胁迫第7天时达到最大值(11.4μg·mg^-1), 而可溶性糖和脯氨酸均呈现先升高后降低的趋势, 分别在高温培养的第3天和第5天时达到最大值; 3) 受到高温胁迫后, 羊栖菜超氧化物歧化酶(SOD)比活性增强, 而过氧化物酶(POD)和过氧化氢酶(CAT)比活性在高温胁迫过程中呈现下降的趋势, 说明羊栖菜主要通过抗氧化酶SOD比活性增强来降低胁迫对藻体带来的伤害。总之, 羊栖菜对高温胁迫比较敏感, 温度越高, 培养时间越长, 羊栖菜受到的伤害越大。

关键词: 羊栖菜, 高温胁迫, 渗透调节物质, 抗氧化酶

Abstract:

In this study, Sargassum fusiforme was treated at 27 ℃ and 32 ℃, and Sargassum fusiforme grown at 22 ℃ was used as control. Air bladders were collected at 0, 1, 3, 5, and 7 days of the treatment, respectively, to explore the effects of high temperature stress on membrane damage, osmotic regulation and antioxidant protection of Sargassum fusiforme. The results showed that the membrane system of Sargassum fusiforme was damaged by high temperature stress, which resulted in ion extravasation in vivo, and membrane lipid peroxidation; and the relative conductivity and Malondialdehyde (MDA) content of cells increased. Soluble protein, soluble sugar and proline were accumulated in the cells. Soluble protein was always higher than the control under high temperature stress, and reached the maximum value at 7 days. Soluble sugar and proline increased first and then decreased in the high temperature stress group, but they were always higher than those in the control group. Soluble sugar reached the maximum value at 3 days under high temperature stress, and proline reached the maximum value at 5 days under high temperature stress. After high temperature stress, Sargassum fusiforme can reduce the damage of high temperature stress mainly by increasing the activity of antioxidant enzyme Superoxide dismutase (SOD), while the activities of Peroxidase (POD) and Catalase (CAT) decreased in the process of high temperature stress. In a word, Sargassum fusiforme is more sensitive to high temperature stress. The higher the temperature is, the longer the treatment time is, the greater the damage to Sargassum fusiforme is.

Key words: Sargassum fusiforme, high temperature stress, osmoregulation substance, antioxidant enzymes

中图分类号:

P735.2

刘丽杰, 林立东. 高温对褐藻羊栖菜逆境生理的影响[J]. 热带海洋学报, 2021, 40(2): 74-82.

LIU Lijie, LIN Lidong. The effect of high temperature on stress physiology of Sargassum fusiforme[J]. Journal of Tropical Oceanography, 2021, 40(2): 74-82.

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高温对褐藻羊栖菜逆境生理的影响

The effect of high temperature on stress physiology of Sargassum fusiforme

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