水母共附生真菌Aspergillus fumigatus SCSIO41214中生物碱类化合物研究

doi:10.11978/2022193

热带海洋学报 ›› 2023, Vol. 42 ›› Issue (4): 195-201.doi: 10.11978/2022193CSTR: 32234.14.2022193

• 海洋资源开发 • 上一篇

水母共附生真菌Aspergillus fumigatus SCSIO41214中生物碱类化合物研究

杨斌(), SALENDRA Limbadri, 刘娟, 刘永宏()

中国科学院热带海洋生物资源生态重点实验室, 广东省海洋药物重点实验室, 中国科学院南海海洋研究所, 广东广州 510301

收稿日期:2022-09-16 修回日期:2022-10-08 出版日期:2023-07-10 发布日期:2022-10-13
作者简介:
杨斌, 男, 副研究员, 从事海洋药物化学研究。email: yangbin@scsio.ac.cn
基金资助:
国家自然科学基金项目(21977102); 广东省基础与应用基础研究基金粤深联合基金(2021B1515120046)

Alkaloids from the Jellyfish-Derived Fungus Aspergillus fumigatus SCSIO41214

YANG Bin(), SALENDRA Limbadri, LIU Juan, LIU Yonghong()

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

Received:2022-09-16 Revised:2022-10-08 Online:2023-07-10 Published:2022-10-13
Supported by:
National Natural Science Foundation of China(21977102); Guangdong-Joint Foundation of Shenzhen(2021B1515120046)

摘要/Abstract

摘要：

从水母共附生真菌Aspergillus fumigatus SCSIO41214中分离得到8个已知生物碱类化合物。这些化合物主要采用包括正相硅胶、反相硅胶等常规柱层析技术, 以及高效液相色谱进行分离纯化。首次通过核磁共振波谱等多种现代波谱技术确定了化合物1的平面结构, 通过单晶衍射技术确定了其相对构型, 化合物2—8是通过波谱分析并结合文献数据比对确定了化合物的结构。体外抗炎活性研究筛选发现, 化合物4在10μmol·L^-1浓度下显示弱的抑制RAW264.7细胞中LPS引起的NO的释放。

关键词: 水母, Aspergillus fumigatus SCSIO41214, 生物碱

Abstract:

Eight known alkaloids were isolated from jellyfish-derived fungus Aspergillus fumigatus SCSIO41214. These compounds were isolated by silica gel column chromatography, ostade-cylsilane (ODS), and high performance liquid chromatography (HPLC). The structure of 1 was identified by performing a comprehensive analysis of spectroscopic data and X-ray diffraction for the first time. The structures of 2 ~ 8 were elucidated by spectroscopic methods and by comparison with data from the literature. In bioassay, compound 4 showed weak inhibitory activity against lipopolysaccharide (LPS)-induced nitric oxide (NO) in RAW264.7 cells at the concentration of 10 μmol·L^-1.

Key words: jellyfish, Aspergillus fumigatus SCSIO41214, alkaloid

杨斌, SALENDRA Limbadri, 刘娟, 刘永宏. 水母共附生真菌Aspergillus fumigatus SCSIO41214中生物碱类化合物研究[J]. 热带海洋学报, 2023, 42(4): 195-201.

YANG Bin, SALENDRA Limbadri, LIU Juan, LIU Yonghong. Alkaloids from the Jellyfish-Derived Fungus Aspergillus fumigatus SCSIO41214[J]. Journal of Tropical Oceanography, 2023, 42(4): 195-201.

图/表 2

参考文献 16

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水母共附生真菌Aspergillus fumigatus SCSIO41214中生物碱类化合物研究

Alkaloids from the Jellyfish-Derived Fungus Aspergillus fumigatus SCSIO41214

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