热带海洋学报 ›› 2009, Vol. 28 ›› Issue (6): 72-78.doi: 10.11978/j.issn.1009-5470.2009.06.072cstr: 32234.14.j.issn.1009-5470.2009.06.072

• 海洋生物学 • 上一篇    下一篇

基于COI序列的翡翠股贻贝Perna viridis线粒体遗传特性分析及其近缘种间的系统关系探讨

位正鹏1, 孔晓瑜1, 2, 吴相云2, 喻子牛2   

  1. 1.中国海洋大学,教育部海水养殖重点实验室, 青岛266003 ; 2.中国科学院南海海洋研究所, 广东省应用海洋生物学重点实验室, 广东 广州 510301
  • 收稿日期:2007-11-09 修回日期:2008-05-04 出版日期:2009-12-30 发布日期:2009-12-12
  • 通讯作者: 喻子牛
  • 作者简介:位正鹏(1982),男,山东省莱阳市人,硕士研究生,主要从事海洋动物分子生物学研究;E-mail: weizhengpeng1234@yahoo.com.cn
  • 基金资助:

    国家自然科学基金项目(30570242);国家“863”计划项目(2006AA10A409)

Genetic analysis of the marine mussel Perna viridis (Bivalvia: Mytilidae) based on mitochondrial COI sequence and its phylogenetic relationship with other congeneric species

WEI Zheng-peng 1,KONG Xiao-yu1, 2 ,WU Xiang-yun2,YU Zi-niu2   

  1. 1.Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China; 2.South China Sea Institute of Oceanology, Chinese Academy of Sciences, Laboratory of Applied Marine Biology,Guangzhou 510301, China
  • Received:2007-11-09 Revised:2008-05-04 Online:2009-12-30 Published:2009-12-12
  • Contact: 喻子牛
  • About author:位正鹏(1982),男,山东省莱阳市人,硕士研究生,主要从事海洋动物分子生物学研究;E-mail: weizhengpeng1234@yahoo.com.cn
  • Supported by:

    国家自然科学基金项目(30570242);国家“863”计划项目(2006AA10A409)

摘要:

应用通用引物 COIL 1490和 COIH 2198对翡翠股贻贝Perna viridis的性腺和体细胞线粒体DNA进行PCR扩增,获得661bp长度的COI基因片段,经过比对性腺与体细胞的COI片段,发现雄性性腺与体细胞COI基因均为一个单倍型,即体内只有一种线粒体DNA类型,没有发现双单性遗传现象,雌、雄性腺的COI基因片段变异率很低(0.31%)。应用PAUP构建了NJ树、MP树以及贝叶斯法构建了贝叶斯树,对股贻贝属3种间的系统关系进行了分析,结果表明,翡翠股贻贝P. viridis与P. canaliculus 和 P. perna 之间的分化与分歧年代的估算是相吻合的。

关键词: 线粒体DNA(mt DNA), 细胞色素氧化酶I亚基基因(COI), 翡翠股贻贝Perna viridis, 双单性遗传(DUI)

Abstract:

Partial mitochondrial COI gene sequences from the gonads (male and female) and somatic tissue of mussel Perna viridis were separately amplified by PCR using the universal primers COIL 1490/COIH 2198. A 661 bp nucleotide sequence of COI gene was obtained. It was found that this gene sequence was identical among different tissues of male individual, and low divergence (0.31%) of COI sequence was detected between male and female of the gonad tissues. Only one haplotype was found in both female and male for the sequence. No doubly uniparental inheritance (DUI) was detected. Based on the COI sequences obtained from this study and those of other Perna species from GenBank, NJ tree, MP tree and Bayesian inference (BI) tree were constructed with PAUP and MrBayes respectively, and phylogenetic relationships among them were analyzed accordingly.

Key words: mitochondrial DNA, COI gene, Perna viridis, doubly uniparental inheritance (DUI)