杂色鲍血细胞免疫特点及免疫功能的研究

doi:10.11978/j.issn.1009-5470.2010.03.071

热带海洋学报 ›› 2010, Vol. 29 ›› Issue (3): 71-76.doi: 10.11978/j.issn.1009-5470.2010.03.071cstr: 32234.14.j.issn.1009-5470.2010.03.071

杂色鲍血细胞免疫特点及免疫功能的研究

王江勇, 郭志勋, 冯娟, 王瑞旋

中国水产科学研究院南海水产研究所, 广东广州 510300

收稿日期:2007-12-21 修回日期:2008-05-04 出版日期:2010-07-01 发布日期:2010-05-24
作者简介:王江勇 (1971—), 男, 湖南省新宁县人, 博士, 副研, 现主要从事海洋生物病害防治研究工作.
基金资助:
广东省自然基金项目资助(04001264); 广东省科技推广项目(2006B40101003); 中央级公益性科研院所基本科研业务费专项资
金资助项目(2007ZD09)；现代农业产业技术体系建设专项资金资助（nycytx-47）

Studies on immune feature and immune function of hemocytes in Haliotis diversicolor Reeve

WANG Jiang-yong, GUO Zhi-xun, FENG Juan, WANG Rui-xuan

South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China

Received:2007-12-21 Revised:2008-05-04 Online:2010-07-01 Published:2010-05-24
About author:王江勇 (1971—), 男, 湖南省新宁县人, 博士, 副研, 现主要从事海洋生物病害防治研究工作.
Supported by:
广东省自然基金项目资助(04001264); 广东省科技推广项目(2006B40101003); 中央级公益性科研院所基本科研业务费专项资
金资助项目(2007ZD09)；现代农业产业技术体系建设专项资金资助（nycytx-47）

摘要/Abstract

摘要：

杂色鲍Haliotis diversicolor感染病毒后血细胞总浓度呈现上升趋势, 颗粒细胞与无颗粒细胞浓度与百分比均呈现不同的变化; 感染鲍抗菌活力在第24h时最高, 说明病毒感染刺激病鲍血细胞产生抗菌活性, 第36h时的抗菌活力大大下降; 感染鲍在第6h时, 溶菌活力大大下降, 且一直持续至36h; 血细胞内的活性氧总体呈明显的上升趋势; 受感染的血细胞出现细胞器空泡化和分解。说明病毒感染对杂色鲍血细胞免疫功能的影响非常明显。

关键词: 病毒感染, 血细胞, 抗菌活力, 溶菌活力, 活性氧, 杂色鲍Haliotis diversicolor

Abstract:

Virus disease has become the vital handicap of the abalone culture in China. The concentration, antibacterial activities, bacteriolysis activities and active oxygen of hemocytes of abalone haliotis diversicolor Reeve were studied after the abalone was infected with virus. The concentration of hemocytes ascended obviously, the concentration of granulocyte and agranulocyte that were the two types of hemocytes ascended as well. The ratio of the granulocyte ascended at 6h and descended afterward. The ratio of the agranulocyte descended at 6h and ascended afterward. Antibacterial activities of the infected abalone reached culmination after 24h, which revealed that virus infection stimulated the diseased abalone to produce anti-microbe activity. Antibacterial activities descended greatly at 36h. Bacteriolysis activities of the infected abalone descended greatly at 6h, and lasted till 36h. The active oxygen ascended obviously as a whole. The organelle of the infected hemocyte was broken down and vocuolisied. The results revealed that the immune function was greatly influenced by virus infection.

王江勇,郭志勋,冯娟,王瑞旋. 杂色鲍血细胞免疫特点及免疫功能的研究[J]. 热带海洋学报, 2010, 29(3): 71-76.

WANG Jiang-yong,GUO Zhi-xun,FENG Juan,WANG Rui-xuan. Studies on immune feature and immune function of hemocytes in Haliotis diversicolor Reeve[J]. Journal of Tropical Oceanography, 2010, 29(3): 71-76.

参考文献

[1] WANG JIANGYONG, GUO ZHIXUN, FENG JUAN, et al. Virus infection in cultured abalone, haliotis diversicolor Reeve in Guangdong province, China
[J]. Journal of Shellfish Research, 2004, 23(4): 1163-1168.
[2] 宋振荣, 纪荣兴, 颜素芬, 等. 引起九孔鲍大量死亡的一种球状病毒[J]. 水产学报, 2000, 24(5): 463-467.
[3] WOOTTON E C, PIPE R K. Structural and functional characterization of the blood cells of the bivalve mullusc,Scrobicularia plana[J]. Fish shellfish Immunol, 2003, 15: 249-262.
[4] CHENG T C, RODICK G E, FOLEY D A, Release of lysozyme from hemolymph cell of Mercenaria mercenario during phagocytosis[J]. J Invertebr Pathol, 1975, 25: 261-265.
[5] BACHERE E, MIALHE E, NOEL D, et.al., Knowledge and research prospects in marine mollusk and crustacean immunology[J]. Aquaculture, 1995, 132: 17-32.
[6] 邢婧, 战文斌, 周丽, 栉孔扇贝(Chlamys f'arreri)血细胞类型及抗菌力的研究[J]. 青岛海洋大学学报, 2003, 33(1): 41-46.
[7] 张剑诚, 张峰, 王吉桥. 皱纹盘鲍血细胞分类及活性氧产生机理的研究[J]. 大连水产学院学报, 2004, 19(3): 182-188.
[8] 陈政强, 陈昌生, 战文斌, 等. 不同类群九孔鲍免疫防御机能的比较[J]. 水产学报, 2004, 28(2): 189-194.
[9] 王江勇, 郭志勋, 冯娟, 等. 病毒感染后杂色鲍部分血清免疫因子的变化[J]. 中国水产科学, 2005, 12(3): 344-347.
[10] 孙虎山, 王宜艳, 杨静, 等. 病毒感染对栉孔扇贝血淋巴中过氧化氢酶和过氧化物酶活力的影响[J]. 水产科学, 2004, 23(8) : 4-6.
[11] Boman H G, Nilsson-Faye I, Paul K, et al. Characteristics of an inducible cell-free antibacterial reaction in hemolymph of Samia cynthia pupae[J]. Infection and Immunity, 1974, 10: 136-145.
[12] Hultmark D, Engström A, Bennich H, et al. Insect immunity: isolation and structure of cecropin D and four minor antibacterial components from Cecropia pupae[J]. European Journal of Biochemistry, 1982, 127: 207-217.
[13] 于仁诚, 谢华. 中国对虾血细胞活性氧指标的初步研究[M]//海洋渔业和水产品加工技术国际研讨会论文集. 山东荣城市, 2001.
[14] 张峰, 李光友. 贝类血细胞活性氧体内防御作用的研究进展[J].海洋科学, 1999, 2: 16-19.
[15] 刘世良, 麦康森. 贝类免疫系统和机理的研究进展[J].海洋学报, 2003, 25 (2): 95-105.
[16] CHENG T C. The role of lysosomes in molluscan inflammation [J]. Smer Zool, 1983, 6(6): 403-412.
[17] 王江勇, 郭志勋, 冯娟, 等. 养殖杂色鲍暴发病超微病理学研究[J]. 海洋科学, 2007, 31(3): 28-32.

杂色鲍血细胞免疫特点及免疫功能的研究

Studies on immune feature and immune function of hemocytes in Haliotis diversicolor Reeve

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