急性盐度胁迫对斜带石斑鱼Na+/K+-ATP酶及血清应激指标的影响

doi:10.11978/j.issn.1009-5470.2010.04.160

热带海洋学报 ›› 2010, Vol. 29 ›› Issue (4): 160-164.doi: 10.11978/j.issn.1009-5470.2010.04.160cstr: 32234.14.j.issn.1009-5470.2010.04.160

急性盐度胁迫对斜带石斑鱼Na+/K+-ATP酶及血清应激指标的影响

杨宇晴1,2, 余德光1, 谢骏1, 郁二蒙1, 李望东3, 王广军1, 王海英1, 龚望宝1

1. 中国水产科学研究院珠江水产研究所, 广东广州510380; 2. 上海海洋大学, 上海 201306; 3. 广东省珠海市水产养殖(淡水)科学技术推广站, 广东珠海 519100

收稿日期:2009-12-04 修回日期:2010-02-04 出版日期:2010-07-31 发布日期:2010-07-29
通讯作者: 余德光, 副研究员。
作者简介:杨宇晴(1985—), 女, 广西省北海市人, 硕士研究生, 从事水产健康养殖研究。E-mail: qingmaggie@163.com
基金资助:
广东省科技计划项目(2008A020800012); 现代渔业产业技术体系建设项目(nycytx-49); 国家“863”项目 (2007AA10Z239); 珠
海市科技项目(c20082031)

Effects of acute salinity stress on Na+/K+-ATPase activity and plasma indicators of Epinephelus coioides

YANG Yu-qing1,2, YU De-guang1, XIE Jun1, YU Er-meng1, LI Wang-dong3, WANG Guang-jun1, WANG Hai-ying1, GONG Wang-bao1

1. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, China; 2. Shanghai Ocean University, Shanghai 201306, China; 3. Zhuhai Aquaculture (Freshwater) Science and Technology Promotion Station of Guangdong, Zhuhai 519100, China

Received:2009-12-04 Revised:2010-02-04 Online:2010-07-31 Published:2010-07-29
Contact: 余德光, 副研究员。
About author:杨宇晴(1985—), 女, 广西省北海市人, 硕士研究生, 从事水产健康养殖研究。E-mail: qingmaggie@163.com
Supported by:
广东省科技计划项目(2008A020800012); 现代渔业产业技术体系建设项目(nycytx-49); 国家“863”项目 (2007AA10Z239); 珠
海市科技项目(c20082031)

摘要/Abstract

摘要：

研究了急性盐度胁迫对斜带石斑鱼幼鱼Epinephelus coioides鳃丝Na+/K+-ATP酶活性和血清应激指标的影响, 将养殖于自然海水(盐度34‰)中, 体重为(19.59±0.25) g的斜带石斑鱼幼鱼直接转移至盐度24‰、14‰、4‰和0‰的水体中, 于转移后1、3、6、12和24h分别检测鳃丝Na+/K+-ATP酶活性和血清中血糖、天门冬氨酸氨基转移酶(AST)、溶菌酶的变化。试验表明: 试验组Na+/K+-ATP酶活性变化基本一致, 均在1h时达到最高值, 随后下降, 至6h达到稳定且均显著高于对照组(P<0.05); 血糖在24‰和14‰盐度组呈下降趋势, 在4‰和0‰盐度组3h时出现最低值, 在6h时达到峰值, 随后逐渐下降; AST水平在24‰和14‰盐度组与对照组无显著差异(P>0.05), 在4‰和0‰盐度组均呈现先上升后下降的趋势, 于6和12h时达到各自峰值; 溶菌酶含量在试验24h时, 在24‰、14‰和4‰盐度组间差异显著(P<0.05), 在0‰盐度组呈现先上升后下降的趋势, 至6h时达到峰值。试验显示, 斜带石斑鱼幼鱼由盐度34‰的水体转移至盐度24‰和14‰的水体后, 其应激强度较弱; 由盐度34‰的水体转移至盐度4‰和0‰的水体后, 其应激反应较大, 适应盐度变化需时也较长。根据本试验结果, 在对斜带石斑鱼进行应激性淡化转运时, 可将其直接从34‰高盐度自然海水中转移至14‰盐度的水体后, 再缓慢降至预定盐度, 从而减少淡化时间。

关键词: 斜带石斑鱼Epinephelus coioides, 盐度胁迫, Na+/K+ -ATP酶, 血糖, 天门冬氨酸氨基转移酶, 溶菌酶

Abstract:

Effects of acute salinity stress on plasma indicators and Na+/K+-ATPase activity in gills of Epinphelus coioides juveniles were studied. E. coioides juveniles with body weight (19.59 ±0.25)g in the seawater with salinity of 34‰ were directly transferred to water with salinities at 0‰, 4‰, 14‰, and 24‰, while those in seawater with salinity of 34‰ were taken as the control group. Plasma glucose content, activities of lysozyme, aspartate carbamyl transferase (AST) and Na+/K+-ATPase in gills were determined at 1, 3, 6, 12, and 24h after the transferring of E. coioides. The results showed that Na+/K+-ATPase activity in salinity stress groups displayed a similar trend of increasing first to its peak at 1h, decreasing thereafter, remaining stable at 6h, and being significantly higher than that in the control group later (P<0.05). Plasma glucose content in waters with salinities 24‰ and 14‰ showed a downward trend, and the content in waters with salinities 4‰ and 0‰ exhibited the lowest value at 3h, followed by an upward trend with the peak occurring at 6h and a gradual decrease thereafter. There were no significant differences in AST among waters with salinity of 14‰, 24‰ and 34‰ (P>0.05). AST in waters with salinities 4‰ and 0‰ reached its peak at 6h and 12h, respective, and then decreased. There were significant differences in lysozyme activity among waters with salinity of 24‰, 14‰ and 4‰ at 24h (P<0.05). Lysozyme activity in salinity 0‰ increased first and then declined, with the peak occurring at 6h. In conclusion, E. coioides juveniles had a strong adaptability to ambient salinity stress, and they could adapt themselves at 6h. In waters with salinity at 14‰ and 24‰, E. coioides juveniles showed the weakest response. According to the results of this study, we conclude that E. coioides juveniles can be transferred directly from seawater to water with salinity of 14‰ before the salinity in water is gradually reduced to a prospective salinity value, which can reduce the time of desalination.

Key words: Epinephelus coioides, salinity stress, Na+/K+ -ATP, plasma glucose, AST, lysozyme

杨宇晴,余德光,谢骏,郁二蒙,李望东,王广军,王海英,龚望宝. 急性盐度胁迫对斜带石斑鱼Na+/K+-ATP酶及血清应激指标的影响[J]. 热带海洋学报, 2010, 29(4): 160-164.

YANG Yu-qing,YU De-guang,XIE Jun,YU Er-meng,LI Wang-dong,WANG Guang-. Effects of acute salinity stress on Na+/K+-ATPase activity and plasma indicators of Epinephelus coioides[J]. Journal of Tropical Oceanography, 2010, 29(4): 160-164.

参考文献

[1] 麦贤杰, 黄伟建, 叶富良, 等. 海水鱼类学繁殖生物学和人工繁育[M]. 北京: 海洋出版社, 2005: 166-167.
[2] JARVIS P L, BALLANTYNE J S. Metabolic responses to salinity acclimation in juvenile shortnose sturgeon Acipenser brevirostrum[J]. Aquaculture, 2003, 219: 891-909.
[3] MARTINEZ-ÁLVAREZ R M, HIDALGO M C, DOMEZAIN A, et al. Physiological changes of sturgeon Acipenser naccarii caused by increasing environmental salinity[J]. The Journal of Experimental Biology, 2002, 205: 3699-3706.
[4] 董燕, 陈立侨, 庄平, 等. 急性盐度胁迫对施氏鲟的皮质醇、代谢反应及渗透调节的影响[J]. 水产学报, 2007, 31(增刊): 38-44.
[5] 徐立文, 苏友禄, 刘广锋, 等. 急性盐度胁迫下军曹鱼稚鱼应激反应的血清学指标[J]. 华南农业大学学报, 2007, 28(2): 91-94.
[6] IMSLAND A K, GUSTAVSSON A, GUNNARSSON S, et al. Effects of reduced salinities on growth, feed conversion efficiency and blood physiology of juvenile Atlantic halibut (Hippoglossus hippoglossus L.)[J]. Aquaculture, 2008, 274: 254-259.
[7] 张海发, 刘晓春, 王云新, 等. 温度、盐度及pH对斜带石斑鱼受精卵孵化和仔鱼活力的影响[J]. 热带海洋学报, 2006, 25(2): 31-36.
[8] BOEUF G, PAYANB P. How should salinity influence fish growth?[J]. Comp Biochem Physiol, Part C, 2001, 130(4): 411-423.
[9] SOENGAS J L, BARCIELA P, ALDEGUNDE M, et al. Gill carbohydrate metabolism of rainbow trout is modified during gradual adaptation to seawater
[J]. J Fish Biol, 1995, 46: 845-856.
[10] 王文博, 李爱华. 环境胁迫对鱼类免疫系统影响的研究概况[J]. 水产学报, 2002, 26(4): 368-374.
[11] 施群焰, 袁重桂. 点带石斑鱼对盐度的适应性研究[J]. 福建水产, 2003, 12(4): 9-11.
[12] 刘冬娥. 斜带石斑鱼早期发育阶段的生物学研究[D]. 集美大学硕士学位论文, 2007.
[13] 刘旭, 丁少雄, 王军. 温度和盐度对斜带石斑鱼幼鱼生长的影响[C]//中国海洋湖沼学会鱼类学分会. 中国动物学会鱼类学分会2004年学术研讨会摘要汇编. 武汉: 中国科学院水生生物研究所, 2004: 56-57.
[14] 黄培卫, 黄国光, 王云新. 斜带石斑鱼网箱养殖技术[J]. 渔业现代化, 2003, 4: 17-18.
[15] 余德光, 谢骏. 网箱养殖海水鱼类疾病防治体系的建立[J]. 中国水产, 2000, 11: 34-36.
[16] SAOUD I P, KREYDIYYEH S, CHALFOUN A, et al. Influence of salinity on survival, growth, plasma osmolality and gill Na+-K+-ATPase activity in the rabbitfish Siganus rivulatus[J]. Journal of Experimental Marine Biology and Ecology, 2007, 348: 183-190.
[17] 潘鲁青, 唐贤明, 刘泓宇, 等. 盐度对褐牙鲆(Paralichthys olivaceus)幼鱼血浆渗透压和鳃丝Na+-K+-ATPase活力的影响[J]. 海洋与湖沼, 2006, 37(1): 1-6.
[18] MCENROE M, JR CECH J J. Osmoregulation in juvenile and adult white sturgeon, Acipenser transmontanus[J]. Environmental Biology of Fishes, 1985, 141: 23-30.
[19] 徐力文, 刘广锋, 王瑞旋, 等. 急性盐度胁迫对军曹鱼稚鱼渗透调节的影响[J]. 应用生态学报, 2007, 18(7): 1596-1600.
[20] EVANS D H, PIERMARINI P M, CHOE K P. The multifunctional fish gill: Dominant site of gas exchange osmoregulation, acid-base regulation, and excretion of nitrogenous waste[J]. Physiological Reviews, 2005, 85: 97-177.
[21] 刘海燕. 环境胁迫和饲料营养对长吻鮠生长、代谢及免疫力的影响[D]. 中国科学院研究生院博士学位论文, 2007.
[22] 于棉荣. 低血糖的预防和救治[J]. 糖尿病新世界, 2009(12): 23-25.
[23] 传良敏, 王文建, 曹万惠, 等. 肝脏疾病患者血清中线粒体型天门冬氨酸氨基转移酶检测的临床意义[J]. 淮海医药, 2009, 27(2): 110-111.
[24] 黄琪琰, 刘丽燕, 范丽萍. 异育银鲫溶血性腹水病的病理生理研究[J]. 水产学报, 1992, 16(4): 316-321.
[25] 刘树清, 江晓路, 牟海津, 等. 免疫多糖对中国对虾血清溶菌酶、磷酸酶和过氧化物的作用[J]. 海洋湖沼, 1999, 30(3): 278-283.
[26] 巍然. 盐度对牙鲆非特异性免疫功能的影响和文昌鱼抗氧化酶的活性[D]. 中国海洋大学硕士学位论文, 2004.

急性盐度胁迫对斜带石斑鱼Na+/K+-ATP酶及血清应激指标的影响

Effects of acute salinity stress on Na+/K+-ATPase activity and plasma indicators of Epinephelus coioides

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